Restriction map for pF1KE0056
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      Bst1107I    
BstEII      BstPI       BstXI       BstZ17I     CelII       
CfoI        ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NsiI        NspI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SfcI        
SfiI        SfoI        SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcyI          643  GACGCC               G[GA]^CG[CT]C
AlwNI         972  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          122  CCTTAGG              CC^T[ACGT]AGG
BanI          231  GGTGCC               G^G[CT][GA]CC
BcnI           88  CCCGG                CC^[GC]GG
BglII         764  AGATCT               A^GATCT
BsaAI         598  TACGTG               [CT]AC^GT[GA]
BsaHI         643  GACGCC               G[GA]^CG[CT]C
BsaI          603  GGTCTC               GGTCTC(1/5)
BseRI         349  CTCCTC               GAGGAG(10/8)
BsmBI         567  CGTCTC               CGTCTC(1/5)
BsmI           43  GAATGC               GAATGC(1/-1)
Bsp1407I      865  TGTACA               T^GTACA
BsrDI         480  CATTGC               GCAATG(2/0)
BsrFI         460  ACCGGC               [GA]^CCGG[CT]
BsrGI         865  TGTACA               T^GTACA
BsrI         1091  ACTGG                ACTGG(1/-1)
BssSI         301  CACGAG               CACGAG(-5/-1)
BstBI         920  TTCGAA               TT^CGAA
Bsu36I        122  CCTTAGG              CC^T[ACGT]AGG
Cfr10I        460  ACCGGC               [GA]^CCGG[CT]
DraI          283  TTTAAA               TTT^AAA
EarI         1287  CTCTTC               CTCTTC(1/4)
Eco81I        122  CCTTAGG              CC^T[ACGT]AGG
EcoNI         185  CCTTGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          643  GACGC                GACGC(5/10)
Hin1I         643  GACGCC               G[GA]^CG[CT]C
Ksp632I      1287  CTCTTC               CTCTTC(1/4)
KspI           13  CCGCGG               CCGC^GG
MspA1I         13  CCGCGG               C[AC]G^C[GT]G
NciI           88  CCCGG                CC^[GC]GG
NcoI          110  CCATGG               C^CATGG
NruI          411  TCGCGA               TCG^CGA
NspV          920  TTCGAA               TT^CGAA
PflMI         726  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII          13  CCGCGG               CCGC^GG
SexAI         227  ACCAGGT              A^CC[AT]GGT
SfuI          920  TTCGAA               TT^CGAA
SgrAI         459  CACCGGCG             C[GA]^CCGG[CT]G
SspBI         865  TGTACA               T^GTACA
SspI         1171  AATATT               AAT^ATT
StuI         1258  AGGCCT               AGG^CCT
TfiI          629  GAATC                G^A[AT]TC
Van91I        726  CCAGAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII          14  CGCG                 CG^CG
              412  CGCG                
AcsI          287  AAATTC               [GA]^AATT[CT]
             1190  AAATTC              
AfaI          866  GTAC                 GT^AC
             1209  GTAC                
ApoI          287  AAATTC               [GA]^AATT[CT]
             1190  AAATTC              
BalI          164  TGGCCA               TGG^CCA
              852  TGGCCA              
BamHI         387  GGATCC               G^GATCC
             1130  GGATCC              
BpmI          520  CTGGAG               CTGGAG(16/14)
             1013  CTGGAG              
BsaWI         390  TCCGGT               [AT]^CCGG[AT]
             1061  ACCGGA              
BsgI          369  CTGCAC               GTGCAG(16/14)
              450  GTGCAG              
BspMI         158  ACCTGC               ACCTGC(4/8)
              319  ACCTGC              
BstAPI        207  GCATCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              225  GCACCAGGTGC         
BstUI          14  CGCG                 CG^CG
              412  CGCG                
DraIII        226  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              307  CACGCTGTG           
EaeI          164  TGGCCA               [CT]^GGCC[GA]
              852  TGGCCA              
MluNI         164  TGGCCA               TGG^CCA
              852  TGGCCA              
MscI          164  TGGCCA               TGG^CCA
              852  TGGCCA              
MslI          325  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              814  CATTCCAATG          
MvnI           14  CGCG                 CG^CG
              412  CGCG                
PleI          942  GACTC                GAGTC(4/5)
              476  GAGTC               
PpuMI         260  AGGACCC              [GA]G^G[AT]CC[CT]
              515  GGGACCT             
RsaI          866  GTAC                 GT^AC
             1209  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BbvI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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