Restriction map for pF1KE0056
 (Linear) (Six-base) MAPSORT of: KIEE0056.seq  Check: 3514  from: 1  to: 1416



KIEE0056.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     167     328    1416 
   Size:        167     161    1088

  Fragments arranged by size: 

                1088     167     161
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     831    1416 
   Size:        831     585
 
AleI CACnn'nnGTG

Cuts at:      0     329    1416 
   Size:        329    1087
 
AlwNI CAG_nnn'CTG

Cuts at:      0     977    1416 
   Size:        977     439
 
ApoI r'AATT_y

Cuts at:      0     287    1190    1416 
   Size:        287     903     226

  Fragments arranged by size: 

                 903     287     226
 
BamHI G'GATC_C

Cuts at:      0     387    1130    1416 
   Size:        387     743     286

  Fragments arranged by size: 

                 743     387     286
 
BanI G'GyrC_C

Cuts at:      0     231    1416 
   Size:        231    1185
 
BglII A'GATC_T

Cuts at:      0     764    1416 
   Size:        764     652
 
Bme1580I G_kGCm'C

Cuts at:      0     302    1416 
   Size:        302    1114
 
BmgBI CAC'GTC

Cuts at:      0     618     701    1416 
   Size:        618      83     715

  Fragments arranged by size: 

                 715     618      83
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1101    1416 
   Size:       1101     315
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541    1034    1416 
   Size:        541     493     382

  Fragments arranged by size: 

                 541     493     382
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     609    1416 
   Size:        609     807
 
BsaAI yAC'GTr

Cuts at:      0     600    1416 
   Size:        600     816
 
BsaHI Gr'CG_yC

Cuts at:      0     644    1416 
   Size:        644     772
 
BsaWI w'CCGG_w

Cuts at:      0     390    1061    1416 
   Size:        390     671     355

  Fragments arranged by size: 

                 671     390     355
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283     923     953    1416 
   Size:        253      30     640      30     463

  Fragments arranged by size: 

                 640     463     253      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340    1416 
   Size:        340    1076
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354     471    1416 
   Size:        354     117     945

  Fragments arranged by size: 

                 945     354     117
 
BsiHKAI G_wGCw'C

Cuts at:      0      22     308     319     425    1416 
   Size:         22     286      11     106     991

  Fragments arranged by size: 

                 991     286     106      22      11
 
BsmI GAATG_Cn'

Cuts at:      0      49    1416 
   Size:         49    1367
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     573    1416 
   Size:        573     843
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     302     308     319     425    1416 
   Size:         22     280       6      11     106     991

  Fragments arranged by size: 

                 991     280     106      22      11       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167     328    1416 
   Size:        167     161    1088

  Fragments arranged by size: 

                1088     167     161
 
BsrDI GCAATG_nn'

Cuts at:      0     479    1416 
   Size:        479     937
 
BsrFI r'CCGG_y

Cuts at:      0     460    1416 
   Size:        460     956
 
BsrGI T'GTAC_A

Cuts at:      0     865    1416 
   Size:        865     551
 
BssSI C'ACGA_G

Cuts at:      0     301    1416 
   Size:        301    1115
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     213     231    1416 
   Size:        213      18    1185

  Fragments arranged by size: 

                1185     213      18
 
BstBI TT'CG_AA

Cuts at:      0     921    1416 
   Size:        921     495
 
BstYI r'GATC_y

Cuts at:      0     275     387     764    1130    1416 
   Size:        275     112     377     366     286

  Fragments arranged by size: 

                 377     366     286     275     112
 
Bsu36I CC'TnA_GG

Cuts at:      0     123    1416 
   Size:        123    1293
 
BtgI C'CryG_G

Cuts at:      0      13     110    1220    1416 
   Size:         13      97    1110     196

  Fragments arranged by size: 

                1110     196      97      13
 
DraI TTT'AAA

Cuts at:      0     285    1416 
   Size:        285    1131
 
DraIII CAC_nnn'GTG

Cuts at:      0     231     312    1416 
   Size:        231      81    1104

  Fragments arranged by size: 

                1104     231      81
 
EaeI y'GGCC_r

Cuts at:      0     164     852    1416 
   Size:        164     688     564

  Fragments arranged by size: 

                 688     564     164
 
EarI CTCTTCn'nnn_

Cuts at:      0    1293    1416 
   Size:       1293     123
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      54    1416 
   Size:         54    1362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     831    1034    1416 
   Size:        541     290     203     382

  Fragments arranged by size: 

                 541     382     290     203
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     189    1416 
   Size:        189    1227
 
EcoO109I rG'GnC_Cy

Cuts at:      0     119     261     509     516    1416 
   Size:        119     142     248       7     900

  Fragments arranged by size: 

                 900     248     142     119       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     253     285     763     795    1339    1371    1416 
   Size:        253      32     478      32     544      32      45

  Fragments arranged by size: 

                 544     478     253      45      32      32      32
 
MscI TGG'CCA

Cuts at:      0     166     854    1416 
   Size:        166     688     562

  Fragments arranged by size: 

                 688     562     166
 
MslI CAynn'nnrTG

Cuts at:      0     329     818    1416 
   Size:        329     489     598

  Fragments arranged by size: 

                 598     489     329
 
MspA1I CmG'CkG

Cuts at:      0      15    1416 
   Size:         15    1401
 
NcoI C'CATG_G

Cuts at:      0     110    1416 
   Size:        110    1306
 
NruI TCG'CGA

Cuts at:      0     413    1416 
   Size:        413    1003
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     732    1416 
   Size:        732     684
 
PpuMI rG'GwC_Cy

Cuts at:      0     261     516    1416 
   Size:        261     255     900

  Fragments arranged by size: 

                 900     261     255
 
SacII CC_GC'GG

Cuts at:      0      16    1416 
   Size:         16    1400
 
SexAI A'CCwGG_T

Cuts at:      0     227    1416 
   Size:        227    1189
 
SgrAI Cr'CCGG_yG

Cuts at:      0     460    1416 
   Size:        460     956
 
SspI AAT'ATT

Cuts at:      0    1173    1416 
   Size:       1173     243
 
StuI AGG'CCT

Cuts at:      0    1260    1416 
   Size:       1260     156
 
StyI C'CwwG_G

Cuts at:      0     110     185    1145    1396    1416 
   Size:        110      75     960     251      20

  Fragments arranged by size: 

                 960     251     110      75      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1083    1318    1416 
   Size:       1083     235      98

  Fragments arranged by size: 

                1083     235      98
 
TatI w'GTAC_w

Cuts at:      0     865    1208    1416 
   Size:        865     343     208

  Fragments arranged by size: 

                 865     343     208
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     676     708     923     955    1416 
   Size:        676      32     215      32     461

  Fragments arranged by size: 

                 676     461     215      32      32
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApoI    BamHI     BanI    BglII
 Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    Hin4I     MscI     MslI   MspA1I     NcoI     NruI    PflMI
    PpuMI    SacII    SexAI    SgrAI     SspI     StuI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI     BmtI     BplI   Bpu10I
    BpuEI    BsaBI    BseYI    BsiEI    BsiWI    BspEI    BspHI    BsrBI
   BssHII   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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