Restriction map for pF1KE0051
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
Aor51HI     AscI        AseI        AsnI        AviII       
AvrII       BbeI        BbrPI       BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     CelII       ClaI        CpoI        
DraI        EagI        EclXI       Eco47III    Eco52I      
EcoRV       FseI        FspI        HaeII       HpaI        
KasI        MfeI        MluI        MroI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I        964  GGTACC               G^GTACC
AccI          385  GTCTAC               GT^[AC][GT]AC
AcsI         1444  GAATTC               [GA]^AATT[CT]
AgeI          676  ACCGGT               A^CCGGT
Alw44I       2625  GTGCAC               G^TGCAC
ApaI         2879  GGGCCC               GGGCC^C
ApaLI        2625  GTGCAC               G^TGCAC
ApoI         1444  GAATTC               [GA]^AATT[CT]
Asp718I       964  GGTACC               G^GTACC
BfaI         2974  CTAG                 C^TAG
BglII        2915  AGATCT               A^GATCT
BsaWI         676  ACCGGT               [AT]^CCGG[AT]
BsmBI        1762  GAGACG               CGTCTC(1/5)
BsmI          987  GAATGC               GAATGC(1/-1)
BspHI        1865  TCATGA               T^CATGA
DraIII       2892  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        1444  GAATTC               G^AATTC
EcoT22I       207  ATGCAT               ATGCA^T
HindIII      1002  AAGCTT               A^AGCTT
KpnI          964  GGTACC               GGTAC^C
KspI          867  CCGCGG               CCGC^GG
MaeI         2974  CTAG                 C^TAG
NsiI          207  ATGCAT               ATGCA^T
PflMI        2926  CCAGTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         676  ACCGGT               A^CCGGT
PvuI         1947  CGATCG               CGAT^CG
RcaI         1865  TCATGA               T^CATGA
SacI          118  GAGCTC               GAGCT^C
SacII         867  CCGCGG               CCGC^GG
SapI         2496  GCTCTTC              GCTCTTC(1/4)
SgrAI         675  CACCGGTG             C[GA]^CCGG[CT]G
StuI          653  AGGCCT               AGG^CCT
Van91I       2926  CCAGTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AhdI          397  GACCGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1468  GACAGAAAGTC         
AocI         1552  CCTGAGG              CC^T[ACGT]AGG
             2350  CCTGAGG             
AspEI         397  GACCGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1468  GACAGAAAGTC         
AspHI         118  GAGCTC               G[AT]GC[AT]^C
             2625  GTGCAC              
BamHI        1853  GGATCC               G^GATCC
             2979  GGATCC              
BbvCI         127  GCTGAGG              CCTCAGC(-5/-2)
             2575  GCTGAGG             
BcgI         2011  CGACGTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              209  GCATTTCCTTCG        
BsaBI        1095  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1863  GATCATGATC          
BsiEI        1566  CGACCG               CG[GA][CT]^CG
             1947  CGATCG              
BsiHKAI       118  GAGCTC               G[AT]GC[AT]^C
             2625  GTGCAC              
BsrBI           8  CCGCTC               CCGCTC(-3/-3)
              325  GAGCGG              
BstEII       2391  GGTGACC              G^GT[ACGT]ACC
             2712  GGTGACC             
BstPI        2391  GGTGACC              G^GT[ACGT]ACC
             2712  GGTGACC             
Bsu36I       1552  CCTGAGG              CC^T[ACGT]AGG
             2350  CCTGAGG             
DrdI          540  GACAGGCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2659  GACTGTGAGGTC        
Eam1105I      397  GACCGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1468  GACAGAAAGTC         
EarI         2497  CTCTTC               CTCTTC(1/4)
              244  GAAGAG              
Eco81I       1552  CCTGAGG              CC^T[ACGT]AGG
             2350  CCTGAGG             
EcoNI        1383  CCTCTCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2130  CCTGAAGAAGG         
EcoO65I      2391  GGTGACC              G^GT[ACGT]ACC
             2712  GGTGACC             
Ksp632I      2497  CTCTTC               CTCTTC(1/4)
              244  GAAGAG              
MaeII        2013  ACGT                 A^CGT
             2303  ACGT                
MamI         1095  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1863  GATCATGATC          
MseI           86  TTAA                 T^TAA
              887  TTAA                
NaeI          646  GCCGGC               GCC^GGC
             1907  GCCGGC              
NgoMIV        646  GCCGGC               G^CCGGC
             1907  GCCGGC              
SmlI         2911  CTCAAG               C^T[CT][GA]AG
             2950  CTCAAG              
SphI          205  GCATGC               GCATG^C
             1793  GCATGC              
Tru9I          86  TTAA                 T^TAA
              887  TTAA                
Tsp509I      1445  AATT                 ^AATT
             1914  AATT                
XcmI          299  CCAATGCCATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              671  CCACCACCGGTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Asp700I     AspI        AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BclI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       Tth111I     TthHB8I     XhoII       
XmaCI       XmaI        XmnI        
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