Restriction map for pF1KE0051
 (Linear) (Six-base) MAPSORT of: KIEE0051.seq  Check: 6858  from: 1  to: 3003



KIEE0051.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     958    3003 
   Size:        958    2045
 
AccI GT'mk_AC

Cuts at:      0     386    3003 
   Size:        386    2617
 
Acc65I G'GTAC_C

Cuts at:      0     964    3003 
   Size:        964    2039
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376    2152    3003 
   Size:        376    1776     851

  Fragments arranged by size: 

                1776     851     376
 
AgeI A'CCGG_T

Cuts at:      0     676    3003 
   Size:        676    2327
 
AhdI GACnn_n'nnGTC

Cuts at:      0     402    1473    3003 
   Size:        402    1071    1530

  Fragments arranged by size: 

                1530    1071     402
 
AleI CACnn'nnGTG

Cuts at:      0     679    1342    1372    1600    2173    3003 
   Size:        679     663      30     228     573     830

  Fragments arranged by size: 

                 830     679     663     573     228      30
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424    3003 
   Size:        424    2579
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2731    2763    3003 
   Size:       2731      32     240

  Fragments arranged by size: 

                2731     240      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     756    2492    2763    3003 
   Size:        756    1736     271     240

  Fragments arranged by size: 

                1736     756     271     240
 
ApaI G_GGCC'C

Cuts at:      0    2883    3003 
   Size:       2883     120
 
ApaLI G'TGCA_C

Cuts at:      0    2625    3003 
   Size:       2625     378
 
ApoI r'AATT_y

Cuts at:      0    1444    3003 
   Size:       1444    1559
 
AvaI C'yCGr_G

Cuts at:      0     425     590     627    1463    1511    1598    1697    2680 
   Size:        425     165      37     836      48      87      99     983

Cuts at:   2680    3003 
   Size:        323

  Fragments arranged by size: 

                 983     836     425     323     165      99      87      48
                  37
 
BamHI G'GATC_C

Cuts at:      0    1853    2979    3003 
   Size:       1853    1126      24

  Fragments arranged by size: 

                1853    1126      24
 
BanI G'GyrC_C

Cuts at:      0     631     685     964    1060    1546    1591    1602    3003 
   Size:        631      54     279      96     486      45      11    1401

  Fragments arranged by size: 

                1401     631     486     279      96      54      45      11
            
 
BanII G_rGCy'C

Cuts at:      0     122     578     859    1828    2042    2405    2470    2480 
   Size:        122     456     281     969     214     363      65      10

Cuts at:   2480    2687    2806    2883    2939    3003 
   Size:        207     119      77      56      64

  Fragments arranged by size: 

                 969     456     363     281     214     207     122     119
                  77      65      64      56      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     145     179     922    2661    3003 
   Size:        145      34     743    1739     342

  Fragments arranged by size: 

                1739     743     342     145      34
 
BbvCI CC'TCA_GC

Cuts at:      0     128    2576    3003 
   Size:        128    2448     427

  Fragments arranged by size: 

                2448     427     128
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     198     232    2000    2034    3003 
   Size:        198      34    1768      34     969

  Fragments arranged by size: 

                1768     969     198      34      34
 
BclI T'GATC_A

Cuts at:      0    1643    1862    1868    3003 
   Size:       1643     219       6    1135

  Fragments arranged by size: 

                1643    1135     219       6
 
BfrBI ATG'CAT

Cuts at:      0     209    3003 
   Size:        209    2794
 
BglI GCCn_nnn'nGGC

Cuts at:      0     349     652    1206    3003 
   Size:        349     303     554    1797

  Fragments arranged by size: 

                1797     554     349     303
 
BglII A'GATC_T

Cuts at:      0    2915    3003 
   Size:       2915      88
 
Bme1580I G_kGCm'C

Cuts at:      0     634     688    1254    1551    1660    1891    2629    2883 
   Size:        634      54     566     297     109     231     738     254

Cuts at:   2883    3003 
   Size:        120

  Fragments arranged by size: 

                 738     634     566     297     254     231     120     109
                  54
 
BmgBI CAC'GTC

Cuts at:      0    2014    3003 
   Size:       2014     989
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1883    2889    3003 
   Size:       1883    1006     114

  Fragments arranged by size: 

                1883    1006     114
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     795    3003 
   Size:        795    2208
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1154    1474    1774    3003 
   Size:        118    1036     320     300    1229

  Fragments arranged by size: 

                1229    1036     320     300     118
 
Bpu10I CC'TnA_GC

Cuts at:      0     128    2576    3003 
   Size:        128    2448     427

  Fragments arranged by size: 

                2448     427     128
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2896    2935    3003 
   Size:       2896      39      68

  Fragments arranged by size: 

                2896      68      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52     241     701    1012    3003 
   Size:         52     189     460     311    1991

  Fragments arranged by size: 

                1991     460     311     189      52
 
BsaBI GATnn'nnATC

Cuts at:      0    1099    1867    3003 
   Size:       1099     768    1136

  Fragments arranged by size: 

                1136    1099     768
 
BsaHI Gr'CG_yC

Cuts at:      0    1559    1847    2111    2120    3003 
   Size:       1559     288     264       9     883

  Fragments arranged by size: 

                1559     883     288     264       9
 
BsaWI w'CCGG_w

Cuts at:      0     676    3003 
   Size:        676    2327
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1152    1182    2203    2233    3003 
   Size:       1152      30    1021      30     770

  Fragments arranged by size: 

                1152    1021     770      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     184    1257    1951    2257    3003 
   Size:        184    1073     694     306     746

  Fragments arranged by size: 

                1073     746     694     306     184
 
BseYI C'CCAG_C

Cuts at:      0    1497    2406    2799    3003 
   Size:       1497     909     393     204

  Fragments arranged by size: 

                1497     909     393     204
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1014    2389    2555    2812    3003 
   Size:       1014    1375     166     257     191

  Fragments arranged by size: 

                1375    1014     257     191     166
 
BsiEI CG_ry'CG

Cuts at:      0    1569    1950    3003 
   Size:       1569     381    1053

  Fragments arranged by size: 

                1569    1053     381
 
BsiHKAI G_wGCw'C

Cuts at:      0     122    2629    3003 
   Size:        122    2507     374

  Fragments arranged by size: 

                2507     374     122
 
BsmI GAATG_Cn'

Cuts at:      0     993    3003 
   Size:        993    2010
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1756    3003 
   Size:       1756    1247
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     578     634     688     859    1254    1551    1660 
   Size:        122     456      56      54     171     395     297     109

Cuts at:   1660    1828    1891    2042    2405    2470    2480    2629    2687 
   Size:        168      63     151     363      65      10     149      58

Cuts at:   2687    2806    2883    2939    3003 
   Size:        119      77      56      64

  Fragments arranged by size: 

                 456     395     363     297     171     168     151     149
                 122     119     109      77      65      64      63      58
                  56      56      54      10
 
BspHI T'CATG_A

Cuts at:      0    1865    3003 
   Size:       1865    1138
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     958    1988    2078    3003 
   Size:        958    1030      90     925

  Fragments arranged by size: 

                1030     958     925      90
 
BsrBI CCG'CTC

Cuts at:      0      10     327    3003 
   Size:         10     317    2676

  Fragments arranged by size: 

                2676     317      10
 
BsrDI GCAATG_nn'

Cuts at:      0     293     638    1266    1895    2150    2736    3003 
   Size:        293     345     628     629     255     586     267

  Fragments arranged by size: 

                 629     628     586     345     293     267     255
 
BsrFI r'CCGG_y

Cuts at:      0     646     676    1153    1617    1907    3003 
   Size:        646      30     477     464     290    1096

  Fragments arranged by size: 

                1096     646     477     464     290      30
 
BstEII G'GTnAC_C

Cuts at:      0    2391    2712    3003 
   Size:       2391     321     291

  Fragments arranged by size: 

                2391     321     291
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     681     954    1344    1752    2061    2418    2694    3003 
   Size:        681     273     390     408     309     357     276     309

  Fragments arranged by size: 

                 681     408     390     357     309     309     276     273
            
 
BstYI r'GATC_y

Cuts at:      0     701    1853    2161    2915    2979    3003 
   Size:        701    1152     308     754      64      24

  Fragments arranged by size: 

                1152     754     701     308      64      24
 
Bsu36I CC'TnA_GG

Cuts at:      0    1553    2351    3003 
   Size:       1553     798     652

  Fragments arranged by size: 

                1553     798     652
 
BtgI C'CryG_G

Cuts at:      0      23      62     849     867     896    2156    2174    2543 
   Size:         23      39     787      18      29    1260      18     369

Cuts at:   2543    2693    3003 
   Size:        150     310

  Fragments arranged by size: 

                1260     787     369     310     150      39      29      23
                  18      18
 
BtsI GCAGTG_nn'

Cuts at:      0     254    2168    2629    2920    3003 
   Size:        254    1914     461     291      83

  Fragments arranged by size: 

                1914     461     291     254      83
 
DraIII CAC_nnn'GTG

Cuts at:      0    2897    3003 
   Size:       2897     106
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     546    2665    3003 
   Size:        546    2119     338

  Fragments arranged by size: 

                2119     546     338
 
EaeI y'GGCC_r

Cuts at:      0     200     668     828    1207    1238    1662    1691    1943 
   Size:        200     468     160     379      31     424      29     252

Cuts at:   1943    2177    3003 
   Size:        234     826

  Fragments arranged by size: 

                 826     468     424     379     252     234     200     160
                  31      29
 
EarI CTCTTCn'nnn_

Cuts at:      0     239    2503    3003 
   Size:        239    2264     500

  Fragments arranged by size: 

                2264     500     239
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1414    3003 
   Size:       1414    1589
 
EcoICRI GAG'CTC

Cuts at:      0     120    3003 
   Size:        120    2883
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     376    1154    1474    1774    2152    3003 
   Size:        118     258     778     320     300     378     851

  Fragments arranged by size: 

                 851     778     378     320     300     258     118
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1387    2134    3003 
   Size:       1387     747     869

  Fragments arranged by size: 

                1387     869     747
 
EcoO109I rG'GnC_Cy

Cuts at:      0     351    2355    2676    2880    3003 
   Size:        351    2004     321     204     123

  Fragments arranged by size: 

                2004     351     321     204     123
 
EcoRI G'AATT_C

Cuts at:      0    1444    3003 
   Size:       1444    1559
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     759    3003 
   Size:        759    2244
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     388     408     420     440     538     570    1459    1491 
   Size:        388      20      12      20      98      32     889      32

Cuts at:   1491    2203    2235    2743    2775    3003 
   Size:        712      32     508      32     228

  Fragments arranged by size: 

                 889     712     508     388     228      98      32      32
                  32      32      20      20      12
 
HincII GTy'rAC

Cuts at:      0     525     600    2595    2850    3003 
   Size:        525      75    1995     255     153

  Fragments arranged by size: 

                1995     525     255     153      75
 
HindIII A'AGCT_T

Cuts at:      0    1002    3003 
   Size:       1002    2001
 
KpnI G_GTAC'C

Cuts at:      0     968    3003 
   Size:        968    2035
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     952    1073    2324    3003 
   Size:        952     121    1251     679

  Fragments arranged by size: 

                1251     952     679     121
 
MscI TGG'CCA

Cuts at:      0     670     830    1240    1693    2179    3003 
   Size:        670     160     410     453     486     824

  Fragments arranged by size: 

                 824     670     486     453     410     160
 
MslI CAynn'nnrTG

Cuts at:      0     679     802    1342    1372    1600    1639    2095    2155 
   Size:        679     123     540      30     228      39     456      60

Cuts at:   2155    2173    2692    2722    3003 
   Size:         18     519      30     281

  Fragments arranged by size: 

                 679     540     519     456     281     228     123      60
                  39      30      30      18
 
MspA1I CmG'CkG

Cuts at:      0     869    1571    1836    1998    2335    2373    2554    2607 
   Size:        869     702     265     162     337      38     181      53

Cuts at:   2607    3003 
   Size:        396

  Fragments arranged by size: 

                 869     702     396     337     265     181     162      53
                  38
 
NaeI GCC'GGC

Cuts at:      0     648    1909    3003 
   Size:        648    1261    1094

  Fragments arranged by size: 

                1261    1094     648
 
NcoI C'CATG_G

Cuts at:      0     849    2156    2693    3003 
   Size:        849    1307     537     310

  Fragments arranged by size: 

                1307     849     537     310
 
NgoMIV G'CCGG_C

Cuts at:      0     646    1907    3003 
   Size:        646    1261    1096

  Fragments arranged by size: 

                1261    1096     646
 
NsiI A_TGCA'T

Cuts at:      0     211    3003 
   Size:        211    2792
 
NspI r_CATG'y

Cuts at:      0     209     621    1797    3003 
   Size:        209     412    1176    1206

  Fragments arranged by size: 

                1206    1176     412     209
 
PasI CC'CwG_GG

Cuts at:      0     439     960    1023    2340    2418    2780    3003 
   Size:        439     521      63    1317      78     362     223

  Fragments arranged by size: 

                1317     521     439     362     223      78      63
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2932    3003 
   Size:       2932      71
 
PfoI T'CCnGG_A

Cuts at:      0    1730    1751    3003 
   Size:       1730      21    1252

  Fragments arranged by size: 

                1730    1252      21
 
PspOMI G'GGCC_C

Cuts at:      0    2879    3003 
   Size:       2879     124
 
PstI C_TGCA'G

Cuts at:      0     483     939    2075    3003 
   Size:        483     456    1136     928

  Fragments arranged by size: 

                1136     928     483     456
 
PvuI CG_AT'CG

Cuts at:      0    1950    3003 
   Size:       1950    1053
 
PvuII CAG'CTG

Cuts at:      0    1836    2373    2554    2607    3003 
   Size:       1836     537     181      53     396

  Fragments arranged by size: 

                1836     537     396     181      53
 
SacI G_AGCT'C

Cuts at:      0     122    3003 
   Size:        122    2881
 
SacII CC_GC'GG

Cuts at:      0     870    3003 
   Size:        870    2133
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2503    3003 
   Size:       2503     500
 
SfcI C'TryA_G

Cuts at:      0     479     935    2071    2598    3003 
   Size:        479     456    1136     527     405

  Fragments arranged by size: 

                1136     527     479     456     405
 
SgrAI Cr'CCGG_yG

Cuts at:      0     676    3003 
   Size:        676    2327
 
SmaI CCC'GGG

Cuts at:      0    1513    1600    1699    3003 
   Size:       1513      87      99    1304

  Fragments arranged by size: 

                1513    1304      99      87
 
SmlI C'TyrA_G

Cuts at:      0    2911    2950    3003 
   Size:       2911      39      53

  Fragments arranged by size: 

                2911      53      39
 
SphI G_CATG'C

Cuts at:      0     209    1797    3003 
   Size:        209    1588    1206

  Fragments arranged by size: 

                1588    1206     209
 
StuI AGG'CCT

Cuts at:      0     655    3003 
   Size:        655    2348
 
StyI C'CwwG_G

Cuts at:      0     656     782     849     902    1271    2156    2471    2693 
   Size:        656     126      67      53     369     885     315     222

Cuts at:   2693    3003 
   Size:        310

  Fragments arranged by size: 

                 885     656     369     315     310     222     126      67
                  53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1771    2370    2410    2521    2656    2772    2908    3003 
   Size:       1771     599      40     111     135     116     136      95

  Fragments arranged by size: 

                1771     599     136     135     116     111      95      40
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1152    1184    3003 
   Size:       1152      32    1819

  Fragments arranged by size: 

                1819    1152      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     433     472     844    3003 
   Size:        433      39     372    2159

  Fragments arranged by size: 

                2159     433     372      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     306     678    3003 
   Size:        306     372    2325

  Fragments arranged by size: 

                2325     372     306
 
XmaI C'CCGG_G

Cuts at:      0    1511    1598    1697    3003 
   Size:       1511      87      99    1306

  Fragments arranged by size: 

                1511    1306      99      87
 
XmnI GAAnn'nnTTC

Cuts at:      0     929    1141    1360    1444    2275    3003 
   Size:        929     212     219      84     831     728

  Fragments arranged by size: 

                 929     831     728     219     212      84
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII  HindIII     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI     PasI
    PflMI     PfoI   PspOMI     PstI     PvuI    PvuII     SacI    SacII
     SapI     SfcI    SgrAI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BciVI     BlpI     BmtI    BsaAI    BsiWI
    BspEI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     EagI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI    RsrII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI     XbaI     XhoI     ZraI
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