Restriction map for pF1KE0050
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# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DsaI        EagI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HapII       HgaI        Hin1I       
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI          292  CCGC                 CCGC(-3/-1)
AflIII        907  ACATGT               A^C[GA][CT]GT
Aor51HI       711  AGCGCT               AGC^GCT
Asp700I       419  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI            9  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          162  TGGCCA               TGG^CCA
BbsI            6  GAAGAC               GAAGAC(2/6)
BcgI         1259  GCAAGACCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          219  TGATCA               T^GATCA
BpuAI           6  GAAGAC               GAAGAC(2/6)
BsmFI          55  GGGAC                GGGAC(10/14)
BspLU11I      907  ACATGT               A^CATGT
BsrDI          59  CATTGC               GCAATG(2/0)
CfoI          712  GCGC                 GCG^C
Cfr13I        389  GGCCC                G^G[ACGT]CC
EaeI          162  TGGCCA               [CT]^GGCC[GA]
Eco47III      711  AGCGCT               AGC^GCT
FbaI          219  TGATCA               T^GATCA
HaeII         711  AGCGCT               [GA]GCGC^[CT]
HhaI          712  GCGC                 GCG^C
HinP1I        712  GCGC                 G^CGC
HincII         18  GTCAAC               GT[CT]^[GA]AC
HindII         18  GTCAAC               GT[CT]^[GA]AC
HindIII       267  AAGCTT               A^AGCTT
HphI          640  GGTGA                GGTGA(8/7)
MaeII          10  ACGT                 A^CGT
MluNI         162  TGGCCA               TGG^CCA
MscI          162  TGGCCA               TGG^CCA
MwoI          285  GCCTGAACCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI           9  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Sau96I        389  GGCCC                G^G[ACGT]CC
SfaNI         279  GCATC                GCATC(5/9)
SphI          228  GCATGC               GCATG^C
Tth111I         9  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          419  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          491  GTATAC               GT^[AC][GT]AC
             1372  GTATAC              
AcsI           91  GAATTT               [GA]^AATT[CT]
              377  GAATTT              
AfaI          206  GTAC                 GT^AC
              463  GTAC                
AlwI           45  GATCC                GGATC(4/5)
              262  GGATC               
ApoI           91  GAATTT               [GA]^AATT[CT]
              377  GAATTT              
BbvI          169  GCAGC                GCAGC(8/12)
              518  GCAGC               
BssKI        1116  CCTGG                ^CC[ACGT]GG
             1200  CCTGG               
Bst1107I      491  GTATAC               GTA^TAC
             1372  GTATAC              
BstNI        1116  CCTGG                CC^[AT]GG
             1200  CCTGG               
BstZ17I       491  GTATAC               GTA^TAC
             1372  GTATAC              
DdeI          357  CTCAG                C^T[ACGT]AG
              552  CTTAG               
DrdI            9  GACGTTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
               68  GACAAGCAAGTC        
EcoRII       1116  CCTGG                ^CC[AT]GG
             1200  CCTGG               
Fnu4HI        169  GCAGC                GC^[ACGT]GC
              518  GCAGC               
ItaI          169  GCAGC                GC^[ACGT]GC
              518  GCAGC               
MvaI         1116  CCTGG                CC^[AT]GG
             1200  CCTGG               
NspI          228  GCATGC               [GA]CATG^[CT]
              907  ACATGT              
RsaI          206  GTAC                 GT^AC
              463  GTAC                
ScrFI        1116  CCTGG                CC^[ACGT]GG
             1200  CCTGG               
TaqI          131  TCGA                 T^CGA
              936  TCGA                
TseI          169  GCAGC                G^C[AT]GC
              518  GCAGC               
TthHB8I       131  TCGA                 T^CGA
              936  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AhdI        AluI        AspEI       BpmI        BsrI        
BstF5I      Cac8I       DpnII       Eam1105I    EarI        
FokI        HaeIII      HinfI       Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MslI        
NdeII       NlaIII      Sau3AI      TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       
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