Restriction map for
pF1KE0045
(Circular) (Six-base) MAPSORT of: pF1KE0045.seq Check: 5365 from: 1 to: 5096
pF1KE0045 5096 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1727 2134 1727
Size: 407 4689
Acc65I G'GTAC_C
Cuts at: 887 2112 887
Size: 1225 3871
AclI AA'CG_TT
Cuts at: 4936 4936
Size: 5096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1733 2921 3353 4100 4368 449
Size: 1284 1188 432 747 268 1177
Fragments arranged by size:
1284 1188 1177 747 432 268
AflII C'TTAA_G
Cuts at: 2082 2082
Size: 5096
AflIII A'CryG_T
Cuts at: 331 562 2448 3552 4529 331
Size: 231 1886 1104 977 898
Fragments arranged by size:
1886 1104 977 898 231
AgeI A'CCGG_T
Cuts at: 3484 3484
Size: 5096
AleI CACnn'nnGTG
Cuts at: 1836 1836
Size: 5096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 800 832 983 1015 800
Size: 32 151 32 4881
Fragments arranged by size:
4881 151 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2401 3968 2401
Size: 1567 3529
ApaLI G'TGCA_C
Cuts at: 1677 3866 1677
Size: 2189 2907
ApoI r'AATT_y
Cuts at: 434 959 1149 2100 4449 4705 434
Size: 525 190 951 2349 256 825
Fragments arranged by size:
2349 951 825 525 256 190
AseI AT'TA_AT
Cuts at: 20 760 20
Size: 740 4356
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
AvaI C'yCGr_G
Cuts at: 2116 2116
Size: 5096
BamHI G'GATC_C
Cuts at: 1360 2121 1360
Size: 761 4335
BanI G'GyrC_C
Cuts at: 887 2112 2526 2774 2809 887
Size: 1225 414 248 35 3174
Fragments arranged by size:
3174 1225 414 248 35
BanII G_rGCy'C
Cuts at: 2110 3140 2110
Size: 1030 4066
BbeI G_GCGC'C
Cuts at: 2530 2778 2530
Size: 248 4848
BbsI GAAGACnn'nnnn_
Cuts at: 318 521 685 318
Size: 203 164 4729
Fragments arranged by size:
4729 203 164
BciVI GTATCCnnnnn_n'
Cuts at: 2994 3755 5096 2994
Size: 761 1341 2994
Fragments arranged by size:
2994 1341 761
BclI T'GATC_A
Cuts at: 1770 2616 1770
Size: 846 4250
BfrBI ATG'CAT
Cuts at: 1088 1195 1088
Size: 107 4989
BglII A'GATC_T
Cuts at: 2611 4648 2611
Size: 2037 3059
BlpI GC'TnA_GC
Cuts at: 2208 2208
Size: 5096
Bme1580I G_kGCm'C
Cuts at: 1681 2721 2814 3870 1681
Size: 1040 93 1056 2907
Fragments arranged by size:
2907 1056 1040 93
BmrI ACTGGGnnnn_n'
Cuts at: 2308 2389 2724 4868 2308
Size: 81 335 2144 2536
Fragments arranged by size:
2536 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3463 3463
Size: 5096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 503 689 338
Size: 165 186 4745
Fragments arranged by size:
4745 186 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2272 3151 3643 3941 2272
Size: 879 492 298 3427
Fragments arranged by size:
3427 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 356 719 4800 356
Size: 363 4081 652
Fragments arranged by size:
4081 652 363
BsaAI yAC'GTr
Cuts at: 332 2449 332
Size: 2117 2979
BsaBI GATnn'nnATC
Cuts at: 6 1967 2610 4647 6
Size: 1961 643 2037 455
Fragments arranged by size:
2037 1961 643 455
BsaHI Gr'CG_yC
Cuts at: 2527 2775 3477 4973 2527
Size: 248 702 1496 2650
Fragments arranged by size:
2650 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2285 2806 3484 3758 3905 4316 2285
Size: 521 678 274 147 411 3065
Fragments arranged by size:
3065 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 800 830 983 1013 1062 1092 1248 1278 1671
Size: 30 153 30 49 30 156 30 393
Cuts at: 1671 1701 800
Size: 30 4195
Fragments arranged by size:
4195 393 156 153 49 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 700 923 1643 1676 1679 1973 700
Size: 223 720 33 3 294 3823
Fragments arranged by size:
3823 720 294 223 33 3
BseYI C'CCAG_C
Cuts at: 2521 3279 3856 4352 2521
Size: 758 577 496 3265
Fragments arranged by size:
3265 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1636 1163
Size: 473 4623
BsiEI CG_ry'CG
Cuts at: 104 2638 2684 3892 104
Size: 2534 46 1208 1308
Fragments arranged by size:
2534 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 748 1681 2110 2888 3078 3870 748
Size: 933 429 778 190 792 1974
Fragments arranged by size:
1974 933 792 778 429 190
BsmI GAATG_Cn'
Cuts at: 2012 2012
Size: 5096
Bsp1286I G_dGCh'C
Cuts at: 748 1681 2110 2721 2814 2888 3078 3140 3870
Size: 933 429 611 93 74 190 62 730
Cuts at: 3870 748
Size: 1974
Fragments arranged by size:
1974 933 730 611 429 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4316 4316
Size: 5096
BspHI T'CATG_A
Cuts at: 831 4272 831
Size: 3441 1655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2146 2360 2662 3043 2146
Size: 214 302 381 4199
Fragments arranged by size:
4199 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3388 3442 4926 5091 50
Size: 3338 54 1484 165 55
Fragments arranged by size:
3338 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1389 2017 3008 1389
Size: 628 991 3477
Fragments arranged by size:
3477 991 628
BsrFI r'CCGG_y
Cuts at: 3094 3275 3484 3094
Size: 181 209 4706
Fragments arranged by size:
4706 209 181
BsrGI T'GTAC_A
Cuts at: 334 334
Size: 5096
BssSI C'ACGA_G
Cuts at: 3367 3725 3367
Size: 358 4738
BstAPI GCAn_nnn'nTGC
Cuts at: 1351 2473 1351
Size: 1122 3974
BstBI TT'CG_AA
Cuts at: 3457 3457
Size: 5096
BstYI r'GATC_y
Cuts at: 1 853 1028 1360 1789 1993 2121 2588 2611
Size: 852 175 332 429 204 128 467 23
Cuts at: 2611 2946 3192 4204 4290 4302 4648 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 852 467 449 429 346 335 332
246 204 175 128 86 23 12
BtgI C'CryG_G
Cuts at: 1032 1218 1032
Size: 186 4910
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3046 3227 3390 3046
Size: 181 163 4752
Fragments arranged by size:
4752 181 163
BtsI GCAGTG_nn'
Cuts at: 1624 2339 1624
Size: 715 4381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1907 1942 1907
Size: 35 5061
DraI TTT'AAA
Cuts at: 234 2095 234
Size: 1861 3235
DrdI GACnn_nn'nnGTC
Cuts at: 2802 3660 2802
Size: 858 4238
EaeI y'GGCC_r
Cuts at: 2681 2855 3246 3273 4618 2681
Size: 174 391 27 1345 3159
Fragments arranged by size:
3159 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2681 2681
Size: 5096
EarI CTCTTCn'nnn_
Cuts at: 164 314 753 1752 3119 3329 164
Size: 150 439 999 1367 210 1931
Fragments arranged by size:
1931 1367 999 439 210 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3304 3614 3760 4906 3304
Size: 310 146 1146 3494
Fragments arranged by size:
3494 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2108 2108
Size: 5096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 449 503 689 1733 2921 3353 4100 4368
Size: 111 54 186 1044 1188 432 747 268
Cuts at: 4368 338
Size: 1066
Fragments arranged by size:
1188 1066 1044 747 432 268 186 111
54
EcoO109I rG'GnC_Cy
Cuts at: 2235 2235
Size: 5096
EcoRI G'AATT_C
Cuts at: 2100 2100
Size: 5096
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 5096
FspI TGC'GCA
Cuts at: 2877 2877
Size: 5096
HaeII r_GCGC'y
Cuts at: 2530 2778 3800 4779 2530
Size: 248 1022 979 2847
Fragments arranged by size:
2847 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 511 543 701 733 1062 1094 1248 1280 2938
Size: 32 158 32 329 32 154 32 1658
Cuts at: 2938 2968 2970 3000 511
Size: 30 2 30 2607
Fragments arranged by size:
2607 1658 329 158 154 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2135 2135
Size: 5096
HindIII A'AGCT_T
Cuts at: 237 237
Size: 5096
KasI G'GCGC_C
Cuts at: 2526 2774 2526
Size: 248 4848
KpnI G_GTAC'C
Cuts at: 891 2116 891
Size: 1225 3871
MluI A'CGCG_T
Cuts at: 4529 4529
Size: 5096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3767 3951 3767
Size: 184 4912
MscI TGG'CCA
Cuts at: 2857 2857
Size: 5096
MslI CAynn'nnrTG
Cuts at: 708 726 1194 1507 1836 3085 3212 708
Size: 18 468 313 329 1249 127 2592
Fragments arranged by size:
2592 1249 468 329 313 127 18
MspA1I CmG'CkG
Cuts at: 667 1975 2207 2521 2881 3894 4139 667
Size: 1308 232 314 360 1013 245 1624
Fragments arranged by size:
1624 1308 1013 360 314 245 232
NaeI GCC'GGC
Cuts at: 3277 3277
Size: 5096
NarI GG'CG_CC
Cuts at: 2527 2775 2527
Size: 248 4848
NcoI C'CATG_G
Cuts at: 1032 1218 1032
Size: 186 4910
NdeI CA'TA_TG
Cuts at: 1191 1191
Size: 5096
NgoMIV G'CCGG_C
Cuts at: 3275 3275
Size: 5096
NsiI A_TGCA'T
Cuts at: 1090 1197 1090
Size: 107 4989
NspI r_CATG'y
Cuts at: 450 566 1199 2149 2649 3556 450
Size: 116 633 950 500 907 1990
Fragments arranged by size:
1990 950 907 633 500 116
PciI A'CATG_T
Cuts at: 562 3552 562
Size: 2990 2106
PmeI GTTT'AAAC
Cuts at: 2095 2095
Size: 5096
PmlI CAC'GTG
Cuts at: 332 332
Size: 5096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 800 832 983 1015 4235 4267 800
Size: 32 151 32 3220 32 1629
Fragments arranged by size:
3220 1629 151 32 32 32
PstI C_TGCA'G
Cuts at: 2143 2828 2143
Size: 685 4411
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5096
PvuII CAG'CTG
Cuts at: 667 1975 2521 2881 667
Size: 1308 546 360 2882
Fragments arranged by size:
2882 1308 546 360
RsrII CG'GwC_CG
Cuts at: 3291 3291
Size: 5096
SacI G_AGCT'C
Cuts at: 2110 2110
Size: 5096
SalI G'TCGA_C
Cuts at: 2133 2133
Size: 5096
SapI GCTCTTCn'nnn_
Cuts at: 314 753 1752 3119 3329 314
Size: 439 999 1367 210 2081
Fragments arranged by size:
2081 1367 999 439 210
SbfI CC_TGCA'GG
Cuts at: 2143 2143
Size: 5096
ScaI AGT'ACT
Cuts at: 4613 4613
Size: 5096
SfcI C'TryA_G
Cuts at: 33 543 2139 2824 3817 4008 33
Size: 510 1596 685 993 191 1121
Fragments arranged by size:
1596 1121 993 685 510 191
SfoI GGC'GCC
Cuts at: 2528 2776 2528
Size: 248 4848
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
SmaI CCC'GGG
Cuts at: 2118 2118
Size: 5096
SmlI C'TyrA_G
Cuts at: 2082 2251 3166 3658 3920 2082
Size: 169 915 492 262 3258
Fragments arranged by size:
3258 915 492 262 169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5096
SphI G_CATG'C
Cuts at: 2149 2649 2149
Size: 500 4596
SspI AAT'ATT
Cuts at: 220 1390 220
Size: 1170 3926
StuI AGG'CCT
Cuts at: 1801 1801
Size: 5096
StyI C'CwwG_G
Cuts at: 361 1032 1218 2230 2584 361
Size: 671 186 1012 354 2873
Fragments arranged by size:
2873 1012 671 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2679 2812 3271 3480 2679
Size: 133 459 209 4295
Fragments arranged by size:
4295 459 209 133
TatI w'GTAC_w
Cuts at: 334 633 1468 1651 4611 334
Size: 299 835 183 2960 819
Fragments arranged by size:
2960 835 819 299 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1352 1384 1671 1703 4613 4645 1352
Size: 32 287 32 2910 32 1803
Fragments arranged by size:
2910 1803 287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2893 2893
Size: 5096
XbaI T'CTAG_A
Cuts at: 2127 2127
Size: 5096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2591 2591
Size: 5096
XmaI C'CCGG_G
Cuts at: 2116 2116
Size: 5096
XmnI GAAnn'nnTTC
Cuts at: 503 594 613 998 503
Size: 91 19 385 4601
Fragments arranged by size:
4601 385 91 19
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI