(Circular) (Six-base) MAPSORT of: pF1KB0989.seq Check: 9398 from: 1 to: 3518 pF1KB0989 3518 bp With 182 enzymes: SgfI * March 2, 2010 14:25 .. AccI GT'mk_AC Cuts at: 556 556 Size: 3518 Acc65I G'GTAC_C Cuts at: 534 534 Size: 3518 AclI AA'CG_TT Cuts at: 3358 3358 Size: 3518 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1343 1775 2522 2790 1343 Size: 432 747 268 2071 Fragments arranged by size: 2071 747 432 268 AflIII A'CryG_T Cuts at: 870 1974 2951 870 Size: 1104 977 1437 Fragments arranged by size: 1437 1104 977 AgeI A'CCGG_T Cuts at: 1906 1906 Size: 3518 AlwNI CAG_nnn'CTG Cuts at: 823 2390 823 Size: 1567 1951 ApaI G_GGCC'C Cuts at: 155 345 155 Size: 190 3328 ApaLI G'TGCA_C Cuts at: 2288 2288 Size: 3518 ApoI r'AATT_y Cuts at: 522 2871 3127 522 Size: 2349 256 913 Fragments arranged by size: 2349 913 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3518 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3518 AvaI C'yCGr_G Cuts at: 166 338 345 538 166 Size: 172 7 193 3146 Fragments arranged by size: 3146 193 172 7 BamHI G'GATC_C Cuts at: 543 543 Size: 3518 BanI G'GyrC_C Cuts at: 126 534 948 1196 1231 126 Size: 408 414 248 35 2413 Fragments arranged by size: 2413 414 408 248 35 BanII G_rGCy'C Cuts at: 155 173 323 345 532 1562 155 Size: 18 150 22 187 1030 2111 Fragments arranged by size: 2111 1030 187 150 22 18 BbeI G_GCGC'C Cuts at: 952 1200 952 Size: 248 3270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3484 BciVI GTATCCnnnnn_n' Cuts at: 1416 2177 3518 1416 Size: 761 1341 1416 Fragments arranged by size: 1416 1341 761 BclI T'GATC_A Cuts at: 1038 1038 Size: 3518 BglII A'GATC_T Cuts at: 1033 3070 1033 Size: 2037 1481 BlpI GC'TnA_GC Cuts at: 203 490 630 203 Size: 287 140 3091 Fragments arranged by size: 3091 287 140 Bme1580I G_kGCm'C Cuts at: 155 345 1143 1236 2292 155 Size: 190 798 93 1056 1381 Fragments arranged by size: 1381 1056 798 190 93 BmrI ACTGGGnnnn_n' Cuts at: 730 811 1146 3290 730 Size: 81 335 2144 958 Fragments arranged by size: 2144 958 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1885 1885 Size: 3518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 337 337 Size: 3518 Bpu10I CC'TnA_GC Cuts at: 147 147 Size: 3518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 694 1573 2065 2363 694 Size: 879 492 298 1849 Fragments arranged by size: 1849 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3222 3222 Size: 3518 BsaAI yAC'GTr Cuts at: 871 871 Size: 3518 BsaBI GATnn'nnATC Cuts at: 6 1032 3069 6 Size: 1026 2037 455 Fragments arranged by size: 2037 1026 455 BsaHI Gr'CG_yC Cuts at: 949 1197 1899 3395 949 Size: 248 702 1496 1072 Fragments arranged by size: 1496 1072 702 248 BsaWI w'CCGG_w Cuts at: 707 1228 1906 2180 2327 2738 707 Size: 521 678 274 147 411 1487 Fragments arranged by size: 1487 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 310 340 310 Size: 30 3488 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 180 180 Size: 3518 BseYI C'CCAG_C Cuts at: 224 943 1701 2278 2774 224 Size: 719 758 577 496 968 Fragments arranged by size: 968 758 719 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 380 380 Size: 3518 BsiEI CG_ry'CG Cuts at: 104 1060 1106 2314 104 Size: 956 46 1208 1308 Fragments arranged by size: 1308 1208 956 46 BsiHKAI G_wGCw'C Cuts at: 173 491 532 1310 1500 2292 173 Size: 318 41 778 190 792 1399 Fragments arranged by size: 1399 792 778 318 190 41 Bsp1286I G_dGCh'C Cuts at: 155 173 323 345 491 532 1143 1236 1310 Size: 18 150 22 146 41 611 93 74 Cuts at: 1310 1500 1562 2292 155 Size: 190 62 730 1381 Fragments arranged by size: 1381 730 611 190 150 146 93 74 62 41 22 18 BspEI T'CCGG_A Cuts at: 2738 2738 Size: 3518 BspHI T'CATG_A Cuts at: 2694 2694 Size: 3518 BspMI ACCTGCnnnn'nnnn_ Cuts at: 568 782 1084 1465 568 Size: 214 302 381 2621 Fragments arranged by size: 2621 381 302 214 BsrBI CCG'CTC Cuts at: 50 202 1810 1864 3348 3513 50 Size: 152 1608 54 1484 165 55 Fragments arranged by size: 1608 1484 165 152 55 54 BsrDI GCAATG_nn' Cuts at: 1430 1430 Size: 3518 BsrFI r'CCGG_y Cuts at: 1516 1697 1906 1516 Size: 181 209 3128 Fragments arranged by size: 3128 209 181 BssSI C'ACGA_G Cuts at: 1789 2147 1789 Size: 358 3160 BstAPI GCAn_nnn'nTGC Cuts at: 895 895 Size: 3518 BstBI TT'CG_AA Cuts at: 1879 1879 Size: 3518 BstYI r'GATC_y Cuts at: 1 446 543 1010 1033 1368 1614 2626 2712 Size: 445 97 467 23 335 246 1012 86 Cuts at: 2712 2724 3070 1 Size: 12 346 449 Fragments arranged by size: 1012 467 449 445 346 335 246 97 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1468 1649 1812 1468 Size: 181 163 3174 Fragments arranged by size: 3174 181 163 BtsI GCAGTG_nn' Cuts at: 761 761 Size: 3518 DraI TTT'AAA Cuts at: 517 517 Size: 3518 DrdI GACnn_nn'nnGTC Cuts at: 1224 2082 1224 Size: 858 2660 EaeI y'GGCC_r Cuts at: 1103 1277 1668 1695 3040 1103 Size: 174 391 27 1345 1581 Fragments arranged by size: 1581 1345 391 174 27 EagI C'GGCC_G Cuts at: 1103 1103 Size: 3518 EarI CTCTTCn'nnn_ Cuts at: 301 1541 1751 301 Size: 1240 210 2068 Fragments arranged by size: 2068 1240 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 315 439 1726 2036 2182 3328 315 Size: 124 1287 310 146 1146 505 Fragments arranged by size: 1287 1146 505 310 146 124 EcoICRI GAG'CTC Cuts at: 171 530 171 Size: 359 3159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 337 1343 1775 2522 2790 337 Size: 1006 432 747 268 1065 Fragments arranged by size: 1065 1006 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 151 152 159 342 657 151 Size: 1 7 183 315 3012 Fragments arranged by size: 3012 315 183 7 1 EcoRI G'AATT_C Cuts at: 522 522 Size: 3518 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1537 1537 Size: 3518 FspI TGC'GCA Cuts at: 463 1299 463 Size: 836 2682 HaeII r_GCGC'y Cuts at: 314 952 1200 2222 3201 314 Size: 638 248 1022 979 631 Fragments arranged by size: 1022 979 638 631 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 310 342 415 447 1360 1390 1392 1422 310 Size: 32 73 32 913 30 2 30 2406 Fragments arranged by size: 2406 913 73 32 32 30 30 2 HincII GTy'rAC Cuts at: 557 557 Size: 3518 KasI G'GCGC_C Cuts at: 948 1196 948 Size: 248 3270 KpnI G_GTAC'C Cuts at: 538 538 Size: 3518 MluI A'CGCG_T Cuts at: 2951 2951 Size: 3518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 198 2189 2373 198 Size: 1991 184 1343 Fragments arranged by size: 1991 1343 184 MscI TGG'CCA Cuts at: 1279 1279 Size: 3518 MslI CAynn'nnrTG Cuts at: 1507 1634 1507 Size: 127 3391 MspA1I CmG'CkG Cuts at: 123 181 207 420 629 943 1303 2316 2561 Size: 58 26 213 209 314 360 1013 245 Cuts at: 2561 123 Size: 1080 Fragments arranged by size: 1080 1013 360 314 245 213 209 58 26 NaeI GCC'GGC Cuts at: 1699 1699 Size: 3518 NarI GG'CG_CC Cuts at: 949 1197 949 Size: 248 3270 NgoMIV G'CCGG_C Cuts at: 1697 1697 Size: 3518 NspI r_CATG'y Cuts at: 254 571 1071 1978 254 Size: 317 500 907 1794 Fragments arranged by size: 1794 907 500 317 PasI CC'CwG_GG Cuts at: 155 212 155 Size: 57 3461 PciI A'CATG_T Cuts at: 1974 1974 Size: 3518 PmeI GTTT'AAAC Cuts at: 517 517 Size: 3518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2657 2689 2657 Size: 32 3486 PspOMI G'GGCC_C Cuts at: 151 341 151 Size: 190 3328 PstI C_TGCA'G Cuts at: 565 1250 565 Size: 685 2833 PvuI CG_AT'CG Cuts at: 104 104 Size: 3518 PvuII CAG'CTG Cuts at: 123 207 943 1303 123 Size: 84 736 360 2338 Fragments arranged by size: 2338 736 360 84 RsrII CG'GwC_CG Cuts at: 1713 1713 Size: 3518 SacI G_AGCT'C Cuts at: 173 532 173 Size: 359 3159 SalI G'TCGA_C Cuts at: 555 555 Size: 3518 SapI GCTCTTCn'nnn_ Cuts at: 301 1541 1751 301 Size: 1240 210 2068 Fragments arranged by size: 2068 1240 210 SbfI CC_TGCA'GG Cuts at: 565 565 Size: 3518 ScaI AGT'ACT Cuts at: 3035 3035 Size: 3518 SfcI C'TryA_G Cuts at: 33 561 1246 2239 2430 33 Size: 528 685 993 191 1121 Fragments arranged by size: 1121 993 685 528 191 SfoI GGC'GCC Cuts at: 950 1198 950 Size: 248 3270 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3518 SmaI CCC'GGG Cuts at: 340 347 540 340 Size: 7 193 3318 Fragments arranged by size: 3318 193 7 SmlI C'TyrA_G Cuts at: 673 1588 2080 2342 673 Size: 915 492 262 1849 Fragments arranged by size: 1849 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3518 SphI G_CATG'C Cuts at: 571 1071 571 Size: 500 3018 StyI C'CwwG_G Cuts at: 652 1006 652 Size: 354 3164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1101 1234 1693 1902 1101 Size: 133 459 209 2717 Fragments arranged by size: 2717 459 209 133 TatI w'GTAC_w Cuts at: 407 3033 407 Size: 2626 892 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3035 3067 3035 Size: 32 3486 Tth111I GACn'n_nGTC Cuts at: 1315 1315 Size: 3518 XbaI T'CTAG_A Cuts at: 549 549 Size: 3518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1013 1013 Size: 3518 XmaI C'CCGG_G Cuts at: 338 345 538 338 Size: 7 193 3318 Fragments arranged by size: 3318 193 7 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI