Restriction map for pF1KB9904
 (Linear) (Six-base) MAPSORT of: KIBB9904.seq  Check: 314  from: 1  to: 1767



KIBB9904.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AccI GT'mk_AC

Cuts at:      0    1295    1767 
   Size:       1295     472
 
Acc65I G'GTAC_C

Cuts at:      0     968    1767 
   Size:        968     799
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     808    1413    1753    1767 
   Size:        808     605     340      14

  Fragments arranged by size: 

                 808     605     340      14
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1522    1767 
   Size:       1522     245
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1344    1767 
   Size:       1344     423
 
ApaI G_GGCC'C

Cuts at:      0     171     959    1004    1066    1767 
   Size:        171     788      45      62     701

  Fragments arranged by size: 

                 788     701     171      62      45
 
ApoI r'AATT_y

Cuts at:      0     709    1237    1767 
   Size:        709     528     530

  Fragments arranged by size: 

                 709     530     528
 
AvaI C'yCGr_G

Cuts at:      0      20    1117    1767 
   Size:         20    1097     650

  Fragments arranged by size: 

                1097     650      20
 
BanI G'GyrC_C

Cuts at:      0     968    1091    1767 
   Size:        968     123     676

  Fragments arranged by size: 

                 968     676     123
 
BanII G_rGCy'C

Cuts at:      0     171     959    1004    1066    1767 
   Size:        171     788      45      62     701

  Fragments arranged by size: 

                 788     701     171      62      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1188    1197    1409    1767 
   Size:       1188       9     212     358

  Fragments arranged by size: 

                1188     358     212       9
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     834    1767 
   Size:        834     933
 
BclI T'GATC_A

Cuts at:      0     834    1767 
   Size:        834     933
 
BglI GCCn_nnn'nGGC

Cuts at:      0     244     736    1767 
   Size:        244     492    1031

  Fragments arranged by size: 

                1031     492     244
 
BglII A'GATC_T

Cuts at:      0     533    1556    1767 
   Size:        533    1023     211

  Fragments arranged by size: 

                1023     533     211
 
BlpI GC'TnA_GC

Cuts at:      0    1754    1767 
   Size:       1754      13
 
Bme1580I G_kGCm'C

Cuts at:      0     171     732     959    1004    1066    1094    1767 
   Size:        171     561     227      45      62      28     673

  Fragments arranged by size: 

                 673     561     227     171      62      45      28
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     298    1077    1767 
   Size:        298     779     690

  Fragments arranged by size: 

                 779     690     298
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1641    1767 
   Size:       1641     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1549    1660    1687    1767 
   Size:         67    1482     111      27      80

  Fragments arranged by size: 

                1482     111      80      67      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     173    1767 
   Size:        173    1594
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166    1767 
   Size:        166    1601
 
BsaHI Gr'CG_yC

Cuts at:      0      36    1767 
   Size:         36    1731
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      80     110    1767 
   Size:         80      30    1657

  Fragments arranged by size: 

                1657      80      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     248    1555    1617    1767 
   Size:        248    1307      62     150

  Fragments arranged by size: 

                1307     248     150      62
 
BseYI C'CCAG_C

Cuts at:      0     501     731    1495    1569    1767 
   Size:        501     230     764      74     198

  Fragments arranged by size: 

                 764     501     230     198      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1492    1767 
   Size:       1492     275
 
BsiEI CG_ry'CG

Cuts at:      0      85     165    1767 
   Size:         85      80    1602

  Fragments arranged by size: 

                1602      85      80
 
BsiHKAI G_wGCw'C

Cuts at:      0    1151    1767 
   Size:       1151     616
 
Bsp1286I G_dGCh'C

Cuts at:      0     171     732     959    1004    1066    1094    1151    1767 
   Size:        171     561     227      45      62      28      57     616

  Fragments arranged by size: 

                 616     561     227     171      62      57      45      28
            
 
BspHI T'CATG_A

Cuts at:      0    1241    1767 
   Size:       1241     526
 
BsrDI GCAATG_nn'

Cuts at:      0     488    1227    1767 
   Size:        488     739     540

  Fragments arranged by size: 

                 739     540     488
 
BsrFI r'CCGG_y

Cuts at:      0     196    1767 
   Size:        196    1571
 
BstEII G'GTnAC_C

Cuts at:      0     496     880    1051    1533    1767 
   Size:        496     384     171     482     234

  Fragments arranged by size: 

                 496     482     384     234     171
 
BstYI r'GATC_y

Cuts at:      0     533    1556    1767 
   Size:        533    1023     211

  Fragments arranged by size: 

                1023     533     211
 
BtgI C'CryG_G

Cuts at:      0     620     960    1265    1767 
   Size:        620     340     305     502

  Fragments arranged by size: 

                 620     502     340     305
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     106     715    1577    1673    1767 
   Size:        106     609     862      96      94

  Fragments arranged by size: 

                 862     609     106      96      94
 
BtsI GCAGTG_nn'

Cuts at:      0     159     464    1748    1767 
   Size:        159     305    1284      19

  Fragments arranged by size: 

                1284     305     159      19
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     852     887    1767 
   Size:        852      35     880

  Fragments arranged by size: 

                 880     852      35
 
EaeI y'GGCC_r

Cuts at:      0     132     162    1582    1767 
   Size:        132      30    1420     185

  Fragments arranged by size: 

                1420     185     132      30
 
EagI C'GGCC_G

Cuts at:      0     162    1767 
   Size:        162    1605
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40     134    1767 
   Size:         40      94    1633

  Fragments arranged by size: 

                1633      94      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     808    1413    1549    1660    1687    1753    1767 
   Size:         67     741     605     136     111      27      66      14

  Fragments arranged by size: 

                 741     605     136     111      67      66      27      14
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355    1767 
   Size:        355    1412
 
EcoO109I rG'GnC_Cy

Cuts at:      0     168     321     955     956    1001    1062    1767 
   Size:        168     153     634       1      45      61     705

  Fragments arranged by size: 

                 705     634     168     153      61      45       1
 
EcoRV GAT'ATC

Cuts at:      0     765    1767 
   Size:        765    1002
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     367    1767 
   Size:        367    1400
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1633    1665    1767 
   Size:       1633      32     102

  Fragments arranged by size: 

                1633     102      32
 
HincII GTy'rAC

Cuts at:      0     513    1767 
   Size:        513    1254
 
KpnI G_GTAC'C

Cuts at:      0     972    1767 
   Size:        972     795
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1379    1767 
   Size:       1379     388
 
MslI CAynn'nnrTG

Cuts at:      0     983    1767 
   Size:        983     784
 
MspA1I CmG'CkG

Cuts at:      0      75     735    1527    1767 
   Size:         75     660     792     240

  Fragments arranged by size: 

                 792     660     240      75
 
NaeI GCC'GGC

Cuts at:      0     198    1767 
   Size:        198    1569
 
NcoI C'CATG_G

Cuts at:      0    1265    1767 
   Size:       1265     502
 
NgoMIV G'CCGG_C

Cuts at:      0     196    1767 
   Size:        196    1571
 
PasI CC'CwG_GG

Cuts at:      0     544    1767 
   Size:        544    1223
 
PfoI T'CCnGG_A

Cuts at:      0     562    1438    1767 
   Size:        562     876     329

  Fragments arranged by size: 

                 876     562     329
 
PspOMI G'GGCC_C

Cuts at:      0     167     955    1000    1062    1767 
   Size:        167     788      45      62     705

  Fragments arranged by size: 

                 788     705     167      62      45
 
SfcI C'TryA_G

Cuts at:      0    1292    1767 
   Size:       1292     475
 
SgrAI Cr'CCGG_yG

Cuts at:      0     196    1767 
   Size:        196    1571
 
SmaI CCC'GGG

Cuts at:      0      22    1767 
   Size:         22    1745
 
SmlI C'TyrA_G

Cuts at:      0     181    1767 
   Size:        181    1586
 
StuI AGG'CCT

Cuts at:      0     361     745    1767 
   Size:        361     384    1022

  Fragments arranged by size: 

                1022     384     361
 
StyI C'CwwG_G

Cuts at:      0     740    1265    1767 
   Size:        740     525     502

  Fragments arranged by size: 

                 740     525     502
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     515    1205    1552    1767 
   Size:        515     690     347     215

  Fragments arranged by size: 

                 690     515     347     215
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112    1767 
   Size:         80      32    1655

  Fragments arranged by size: 

                1655      80      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1192    1767 
   Size:       1192     575
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     387    1272    1767 
   Size:        387     885     495

  Fragments arranged by size: 

                 885     495     387
 
XmaI C'CCGG_G

Cuts at:      0      20    1767 
   Size:         20    1747
 
XmnI GAAnn'nnTTC

Cuts at:      0     823    1600    1767 
   Size:        823     777     167

  Fragments arranged by size: 

                 823     777     167
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrDI
    BsrFI   BstEII    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI
     EagI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    Hin4I
   HincII     KpnI     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     PasI     PfoI   PspOMI     SfcI    SgrAI     SmaI     SmlI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI     BsaI
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EarI  EcoICRI    EcoRI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI
     MluI     MscI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     XbaI     XhoI     ZraI
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