Restriction map for pF1KB9481
##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AatII       AflII       AgeI        Aor51HI     AscI        
AseI        AsnI        BfrI        BglI        BsiWI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     CpoI        DraIII      Eco47III    EcoNI       
FseI        HpaI        MfeI        MluI        MunI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
PvuI        RsrII       SbfI        SfiI        SfuI        
SgrAI       SnaBI       Sse8387I    SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       4913  GGTACC               G^GTACC
AccIII       1060  TCCGGA               T^CCGGA
AcyI         2652  GGCGCC               G[GA]^CG[CT]C
AflIII       3536  ACACGT               A^C[GA][CT]GT
AocI         3924  CCTGAGG              CC^T[ACGT]AGG
Asp718I      4913  GGTACC               G^GTACC
AvaI         6336  CCCGGG               C^[CT]CG[GA]G
AvrII        5316  CCTAGG               C^CTAGG
BbeI         2652  GGCGCC               GGCGC^C
BbrPI        4491  CACGTG               CAC^GTG
BbvCI        2631  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2902  GTATCC               GTATCC(6/5)
BlnI         5316  CCTAGG               C^CTAGG
BsaHI        2652  GGCGCC               G[GA]^CG[CT]C
BseAI        1060  TCCGGA               T^CCGGA
BsoBI        6336  CCCGGG               C^[CT]CG[GA]G
BspEI        1060  TCCGGA               T^CCGGA
Bsu36I       3924  CCTGAGG              CC^T[ACGT]AGG
DraI         1687  TTTAAA               TTT^AAA
EagI         4940  CGGCCG               C^GGCCG
EclXI        4940  CGGCCG               C^GGCCG
Eco52I       4940  CGGCCG               C^GGCCG
Eco81I       3924  CCTGAGG              CC^T[ACGT]AGG
EcoRV        3733  GATATC               GAT^ATC
EcoT22I      3668  ATGCAT               ATGCA^T
HaeII        2652  GGCGCC               [GA]GCGC^[CT]
HgaI         4601  GACGC                GACGC(5/10)
Hin1I        2652  GGCGCC               G[GA]^CG[CT]C
HindIII      5293  AAGCTT               A^AGCTT
KasI         2652  GGCGCC               G^GCGCC
KpnI         4913  GGTACC               GGTAC^C
KspI          225  CCGCGG               CCGC^GG
MroI         1060  TCCGGA               T^CCGGA
NarI         2652  GGCGCC               GG^CGCC
NdeI         1076  CATATG               CA^TATG
NsiI         3668  ATGCAT               ATGCA^T
PmaCI        4491  CACGTG               CAC^GTG
PmlI         4491  CACGTG               CAC^GTG
SacII         225  CCGCGG               CCGC^GG
SapI         3213  GCTCTTC              GCTCTTC(1/4)
ScaI         1138  AGTACT               AGT^ACT
SfoI         2652  GGCGCC               GGC^GCC
SmaI         6336  CCCGGG               CCC^GGG
XcmI         6431  CCAAAGACTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        6336  CCCGGG               C^CCGGG
XmaI         6336  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AclI         1223  AACGTT               AA^CGTT
             6034  AACGTT              
AhdI         2355  GACCAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6498  GACTGAGTGTC         
Alw44I       6358  GTGCAC               G^TGCAC
             6460  GTGCAC              
ApaI         4501  GGGCCC               GGGCC^C
             6346  GGGCCC              
ApaLI        6358  GTGCAC               G^TGCAC
             6460  GTGCAC              
AspEI        2355  GACCAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6498  GACTGAGTGTC         
AspI         2848  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3165  GACTTGGTC           
AviII        3134  TGCGCA               TGC^GCA
             3767  TGCGCA              
BcgI          274  CGATGGACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              463  GCAGAAGTTTCG        
BclI          288  TGATCA               T^GATCA
             2931  TGATCA              
BglII        3813  AGATCT               A^GATCT
             4395  AGATCT              
BlpI          239  GCTCAGC              GC^T[ACGT]AGC
             3987  GCTCAGC             
Bpu1102I      239  GCTCAGC              GC^T[ACGT]AGC
             3987  GCTCAGC             
BsaAI        4366  TACGTG               [CT]AC^GT[GA]
             4491  CACGTG              
BsmBI        2393  GAGACG               CGTCTC(1/5)
             3452  GAGACG              
BspDI        3272  ATCGAT               AT^CGAT
             5846  ATCGAT              
BsrBI         237  CCGCTC               CCGCTC(-3/-3)
             2833  GAGCGG              
BstAPI       3661  GCAGACAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4539  GCAGTGGTTGC         
BstEII       5677  GGTAACC              G^GT[ACGT]ACC
             6054  GGTGACC             
BstPI        5677  GGTAACC              G^GT[ACGT]ACC
             6054  GGTGACC             
CelII         239  GCTCAGC              GC^T[ACGT]AGC
             3987  GCTCAGC             
ClaI         3272  ATCGAT               AT^CGAT
             5846  ATCGAT              
DrdI         4051  GACCCACTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6262  GACCATGGAGTC        
Eam1105I     2355  GACCAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6498  GACTGAGTGTC         
EcoO65I      5677  GGTAACC              G^GT[ACGT]ACC
             6054  GGTGACC             
FbaI          288  TGATCA               T^GATCA
             2931  TGATCA              
FspI         3134  TGCGCA               TGC^GCA
             3767  TGCGCA              
NaeI         4742  GCCGGC               GCC^GGC
             4942  GCCGGC              
NgoMIV       4742  GCCGGC               G^CCGGC
             4942  GCCGGC              
PflFI        2848  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3165  GACTTGGTC           
Psp1406I     1223  AACGTT               AA^CGTT
             6034  AACGTT              
SacI         3985  GAGCTC               GAGCT^C
             4040  GAGCTC              
SexAI        2225  ACCTGGT              A^CC[AT]GGT
             4916  ACCTGGT             
SpeI         1503  ACTAGT               A^CTAGT
             4056  ACTAGT              
SphI         2913  GCATGC               GCATG^C
             5546  GCATGC              
SspI         1192  AATATT               AAT^ATT
             3388  AATATT              
Tth111I      2848  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3165  GACTTGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaBI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspHI       BspMI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SalI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI        
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