Restriction map for pF1KB9481
 (Linear) (Six-base) MAPSORT of: KIBB9481.seq  Check: 9019  from: 1  to: 6645



KIBB9481.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AccI GT'mk_AC

Cuts at:      0     673    1707    3191    6515    6645 
   Size:        673    1034    1484    3324     130

  Fragments arranged by size: 

                3324    1484    1034     673     130
 
Acc65I G'GTAC_C

Cuts at:      0    4913    6645 
   Size:       4913    1732
 
AclI AA'CG_TT

Cuts at:      0    1224    6035    6645 
   Size:       1224    4811     610

  Fragments arranged by size: 

                4811    1224     610
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     722    1558    2276    2733    2808    3622    3911 
   Size:         27     695     836     718     457      75     814     289

Cuts at:   3911    5176    5386    5449    5940    6645 
   Size:       1265     210      63     491     705

  Fragments arranged by size: 

                1265     836     814     718     705     695     491     457
                 289     210      75      63      27
 
AflIII A'CryG_T

Cuts at:      0    3536    6645 
   Size:       3536    3109
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2360    6503    6645 
   Size:       2360    4143     142

  Fragments arranged by size: 

                4143    2360     142
 
AleI CACnn'nnGTG

Cuts at:      0    5018    5122    5881    6355    6365    6645 
   Size:       5018     104     759     474      10     280

  Fragments arranged by size: 

                5018     759     474     280     104      10
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3725    6383    6645 
   Size:       3725    2658     262

  Fragments arranged by size: 

                3725    2658     262
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2314    2845    3115    3643    3913    4177    4246    6291 
   Size:       2314     531     270     528     270     264      69    2045

Cuts at:   6291    6645 
   Size:        354

  Fragments arranged by size: 

                2314    2045     531     528     354     270     270     264
                  69
 
ApaI G_GGCC'C

Cuts at:      0    4505    6350    6645 
   Size:       4505    1845     295

  Fragments arranged by size: 

                4505    1845     295
 
ApaLI G'TGCA_C

Cuts at:      0    6358    6460    6645 
   Size:       6358     102     185

  Fragments arranged by size: 

                6358     185     102
 
ApoI r'AATT_y

Cuts at:      0      10     791    1127    2013    2022    2209    2283    2401 
   Size:         10     781     336     886       9     187      74     118

Cuts at:   2401    3308    3805    5347    5434    6645 
   Size:        907     497    1542      87    1211

  Fragments arranged by size: 

                1542    1211     907     886     781     497     336     187
                 118      87      74      10       9
 
AvaI C'yCGr_G

Cuts at:      0    6336    6645 
   Size:       6336     309
 
AvrII C'CTAG_G

Cuts at:      0    5316    6645 
   Size:       5316    1329
 
BamHI G'GATC_C

Cuts at:      0    1373    1880    4778    6645 
   Size:       1373     507    2898    1867

  Fragments arranged by size: 

                2898    1867    1373     507
 
BanI G'GyrC_C

Cuts at:      0     267    2229    2652    3950    4913    5995    6645 
   Size:        267    1962     423    1298     963    1082     650

  Fragments arranged by size: 

                1962    1298    1082     963     650     423     267
 
BanII G_rGCy'C

Cuts at:      0     124     802    1967    3989    4044    4505    5498    6350 
   Size:        124     678    1165    2022      55     461     993     852

Cuts at:   6350    6645 
   Size:        295

  Fragments arranged by size: 

                2022    1165     993     852     678     461     295     124
                  55
 
BbeI G_GCGC'C

Cuts at:      0    2656    6645 
   Size:       2656    3989
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1024    1983    4560    4609    4973    5045    6645 
   Size:       1024     959    2577      49     364      72    1600

  Fragments arranged by size: 

                2577    1600    1024     959     364      72      49
 
BbvCI CC'TCA_GC

Cuts at:      0    2632    6645 
   Size:       2632    4013
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     263     297     452     486    6645 
   Size:        263      34     155      34    6159

  Fragments arranged by size: 

                6159     263     155      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2913    6645 
   Size:       2913    3732
 
BclI T'GATC_A

Cuts at:      0     288    2931    6645 
   Size:        288    2643    3714

  Fragments arranged by size: 

                3714    2643     288
 
BfrBI ATG'CAT

Cuts at:      0    3670    6645 
   Size:       3670    2975
 
BglII A'GATC_T

Cuts at:      0    3813    4395    6645 
   Size:       3813     582    2250

  Fragments arranged by size: 

                3813    2250     582
 
BlpI GC'TnA_GC

Cuts at:      0     240    3988    6645 
   Size:        240    3748    2657

  Fragments arranged by size: 

                3748    2657     240
 
Bme1580I G_kGCm'C

Cuts at:      0     859    4505    4904    6350    6362    6464    6645 
   Size:        859    3646     399    1446      12     102     181

  Fragments arranged by size: 

                3646    1446     859     399     181     102      12
 
BmgBI CAC'GTC

Cuts at:      0    3525    5790    6645 
   Size:       3525    2265     855

  Fragments arranged by size: 

                3525    2265     855
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1875    4439    6054    6279    6645 
   Size:       1875    2564    1615     225     366

  Fragments arranged by size: 

                2564    1875    1615     366     225
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5905    6559    6645 
   Size:       5905     654      86

  Fragments arranged by size: 

                5905     654      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     330     675     747    1235    1250    1822    1880 
   Size:        316      14     345      72     488      15     572      58

Cuts at:   1880    2050    2442    2942    3571    4015    4254    4882    5212 
   Size:        170     392     500     629     444     239     628     330

Cuts at:   5212    6400    6645 
   Size:       1188     245

  Fragments arranged by size: 

                1188     629     628     572     500     488     444     392
                 345     330     316     245     239     170      72      58
                  15      14
 
Bpu10I CC'TnA_GC

Cuts at:      0    2632    6645 
   Size:       2632    4013
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    2476    4028    6645 
   Size:        325    2151    1552    2617

  Fragments arranged by size: 

                2617    2151    1552     325
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1835    2159    2458    6645 
   Size:       1835     324     299    4187

  Fragments arranged by size: 

                4187    1835     324     299
 
BsaAI yAC'GTr

Cuts at:      0    4368    4493    6645 
   Size:       4368     125    2152

  Fragments arranged by size: 

                4368    2152     125
 
BsaBI GATnn'nnATC

Cuts at:      0    3844    4025    5906    6645 
   Size:       3844     181    1881     739

  Fragments arranged by size: 

                3844    1881     739     181
 
BsaHI Gr'CG_yC

Cuts at:      0    2653    6645 
   Size:       2653    3992
 
BsaWI w'CCGG_w

Cuts at:      0    1060    2614    5681    6645 
   Size:       1060    1554    3067     964

  Fragments arranged by size: 

                3067    1554    1060     964
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     452     482    2588    2618    2852    2882    4321    4351 
   Size:        452      30    2106      30     234      30    1439      30

Cuts at:   4351    5194    5224    5257    5287    6645 
   Size:        843      30      33      30    1358

  Fragments arranged by size: 

                2106    1439    1358     843     452     234      33      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     492    4991    5791    6645 
   Size:        492    4499     800     854

  Fragments arranged by size: 

                4499     854     800     492
 
BseYI C'CCAG_C

Cuts at:      0    3906    4170    6343    6645 
   Size:       3906     264    2173     302

  Fragments arranged by size: 

                3906    2173     302     264
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     619    4954    6645 
   Size:        179     440    4335    1691

  Fragments arranged by size: 

                4335    1691     440     179
 
BsiEI CG_ry'CG

Cuts at:      0    1707    2619    4943    6645 
   Size:       1707     912    2324    1702

  Fragments arranged by size: 

                2324    1707    1702     912
 
BsiHKAI G_wGCw'C

Cuts at:      0     484    3148    3536    3989    4044    6362    6464    6645 
   Size:        484    2664     388     453      55    2318     102     181

  Fragments arranged by size: 

                2664    2318     484     453     388     181     102      55
            
 
BsmI GAATG_Cn'

Cuts at:      0    2984    3478    4822    5393    5627    6645 
   Size:       2984     494    1344     571     234    1018

  Fragments arranged by size: 

                2984    1344    1018     571     494     234
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2387    3446    6645 
   Size:       2387    1059    3199

  Fragments arranged by size: 

                3199    2387    1059
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     484     802     859    1967    3148    3536    3989 
   Size:        124     360     318      57    1108    1181     388     453

Cuts at:   3989    4044    4505    4904    5498    6350    6362    6464    6645 
   Size:         55     461     399     594     852      12     102     181

  Fragments arranged by size: 

                1181    1108     852     594     461     453     399     388
                 360     318     181     124     102      57      55      12
            
 
BspEI T'CCGG_A

Cuts at:      0    1060    6645 
   Size:       1060    5585
 
BspHI T'CATG_A

Cuts at:      0    2691    3575    4889    5729    6645 
   Size:       2691     884    1314     840     916

  Fragments arranged by size: 

                2691    1314     916     884     840
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      40     289    4742    6645 
   Size:         40     249    4453    1903

  Fragments arranged by size: 

                4453    1903     249      40
 
BsrBI CCG'CTC

Cuts at:      0     239    2835    6645 
   Size:        239    2596    3810

  Fragments arranged by size: 

                3810    2596     239
 
BsrDI GCAATG_nn'

Cuts at:      0    2720    3130    4466    4737    4763    5813    6645 
   Size:       2720     410    1336     271      26    1050     832

  Fragments arranged by size: 

                2720    1336    1050     832     410     271      26
 
BsrFI r'CCGG_y

Cuts at:      0    1030    1349    4742    4942    6645 
   Size:       1030     319    3393     200    1703

  Fragments arranged by size: 

                3393    1703    1030     319     200
 
BsrGI T'GTAC_A

Cuts at:      0    3038    3336    4010    4100    6645 
   Size:       3038     298     674      90    2545

  Fragments arranged by size: 

                3038    2545     674     298      90
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3667    4545    6645 
   Size:       3667     878    2100

  Fragments arranged by size: 

                3667    2100     878
 
BstEII G'GTnAC_C

Cuts at:      0    5677    6054    6645 
   Size:       5677     377     591

  Fragments arranged by size: 

                5677     591     377
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     354    1330    4860    4959    6645 
   Size:        354     976    3530      99    1686

  Fragments arranged by size: 

                3530    1686     976     354      99
 
BstYI r'GATC_y

Cuts at:      0     978    1373    1880    2540    3123    3203    3813    3845 
   Size:        978     395     507     660     583      80     610      32

Cuts at:   3845    4395    4630    4699    4778    6624    6645 
   Size:        550     235      69      79    1846      21

  Fragments arranged by size: 

                1846     978     660     610     583     550     507     395
                 235      80      79      69      32      21
 
Bsu36I CC'TnA_GG

Cuts at:      0    3925    6645 
   Size:       3925    2720
 
BtgI C'CryG_G

Cuts at:      0     225    1323    1520    2513    4757    5595    6264    6645 
   Size:        225    1098     197     993    2244     838     669     381

  Fragments arranged by size: 

                2244    1098     993     838     669     381     225     197
            
 
BtsI GCAGTG_nn'

Cuts at:      0     157     837    1172    1772    4546    5151    5375    6360 
   Size:        157     680     335     600    2774     605     224     985

Cuts at:   6360    6372    6464    6645 
   Size:         12      92     181

  Fragments arranged by size: 

                2774     985     680     605     600     335     224     181
                 157      92      12
 
ClaI AT'CG_AT

Cuts at:      0    3273    5847    6645 
   Size:       3273    2574     798

  Fragments arranged by size: 

                3273    2574     798
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    4746    4781    6645 
   Size:       4746      35    1864

  Fragments arranged by size: 

                4746    1864      35
 
DraI TTT'AAA

Cuts at:      0    1689    6645 
   Size:       1689    4956
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    4057    6268    6645 
   Size:       4057    2211     377

  Fragments arranged by size: 

                4057    2211     377
 
EaeI y'GGCC_r

Cuts at:      0     206    2339    4484    4760    4940    6100    6256    6645 
   Size:        206    2133    2145     276     180    1160     156     389

  Fragments arranged by size: 

                2145    2133    1160     389     276     206     180     156
            
 
EagI C'GGCC_G

Cuts at:      0    4940    6645 
   Size:       4940    1705
 
EarI CTCTTCn'nnn_

Cuts at:      0     225     698     947    1894    2295    2550    3220    5955 
   Size:        225     473     249     947     401     255     670    2735

Cuts at:   5955    6645 
   Size:        690

  Fragments arranged by size: 

                2735     947     690     670     473     401     255     249
                 225
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     733     763    3642    6223    6645 
   Size:        733      30    2879    2581     422

  Fragments arranged by size: 

                2879    2581     733     422      30
 
EcoICRI GAG'CTC

Cuts at:      0    3987    4042    6645 
   Size:       3987      55    2603

  Fragments arranged by size: 

                3987    2603      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     316     330     675     722     747    1235    1250 
   Size:         27     289      14     345      47      25     488      15

Cuts at:   1250    1558    1822    1880    2050    2276    2442    2733    2808 
   Size:        308     264      58     170     226     166     291      75

Cuts at:   2808    2942    3571    3622    3911    4015    4254    4882    5176 
   Size:        134     629      51     289     104     239     628     294

Cuts at:   5176    5212    5386    5449    5940    6400    6645 
   Size:         36     174      63     491     460     245

  Fragments arranged by size: 

                 629     628     491     488     460     345     308     294
                 291     289     289     264     245     239     226     174
                 170     166     134     104      75      63      58      51
                  47      36      27      25      15      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3596    4320    4501    4502    5424    6132    6645 
   Size:       3596     724     181       1     922     708     513

  Fragments arranged by size: 

                3596     922     724     708     513     181       1
 
EcoRI G'AATT_C

Cuts at:      0    2022    2209    5434    6645 
   Size:       2022     187    3225    1211

  Fragments arranged by size: 

                3225    2022    1211     187
 
EcoRV GAT'ATC

Cuts at:      0    3735    6645 
   Size:       3735    2910
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     319    3543    6024    6645 
   Size:        319    3224    2481     621

  Fragments arranged by size: 

                3224    2481     621     319
 
FspI TGC'GCA

Cuts at:      0    3136    3769    6645 
   Size:       3136     633    2876

  Fragments arranged by size: 

                3136    2876     633
 
HaeII r_GCGC'y

Cuts at:      0    2656    6645 
   Size:       2656    3989
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     341     373     609     641     927     959    1815    1847 
   Size:        341      32     236      32     286      32     856      32

Cuts at:   1847    2346    2378    3719    3751    4104    4136    4620    4652 
   Size:        499      32    1341      32     353      32     484      32

Cuts at:   4652    5514    5546    5583    5615    5899    5931    6489    6521 
   Size:        862      32      37      32     284      32     558      32

Cuts at:   6521    6614    6645 
   Size:         93      31

  Fragments arranged by size: 

                1341     862     856     558     499     484     353     341
                 286     284     236      93      37      32      32      32
                  32      32      32      32      32      32      32      32
                  32      31
 
HincII GTy'rAC

Cuts at:      0     405     674    1708    3192    3501    4050    5073    6645 
   Size:        405     269    1034    1484     309     549    1023    1572

  Fragments arranged by size: 

                1572    1484    1034    1023     549     405     309     269
            
 
HindIII A'AGCT_T

Cuts at:      0    5293    6645 
   Size:       5293    1352
 
KasI G'GCGC_C

Cuts at:      0    2652    6645 
   Size:       2652    3993
 
KpnI G_GTAC'C

Cuts at:      0    4917    6645 
   Size:       4917    1728
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     239    5774    6645 
   Size:        239    5535     871

  Fragments arranged by size: 

                5535     871     239
 
MscI TGG'CCA

Cuts at:      0     208    2341    4486    6102    6258    6645 
   Size:        208    2133    2145    1616     156     387

  Fragments arranged by size: 

                2145    2133    1616     387     208     156
 
MslI CAynn'nnrTG

Cuts at:      0     273     888    1388    1462    1921    2087    2429    4891 
   Size:        273     615     500      74     459     166     342    2462

Cuts at:   4891    4930    5018    5020    5122    5218    5551    5881    6049 
   Size:         39      88       2     102      96     333     330     168

Cuts at:   6049    6355    6365    6527    6645 
   Size:        306      10     162     118

  Fragments arranged by size: 

                2462     615     500     459     342     333     330     306
                 273     168     166     162     118     102      96      88
                  74      39      10       2
 
MspA1I CmG'CkG

Cuts at:      0     227     257    1207    1730    3210    3593    3913    3965 
   Size:        227      30     950     523    1480     383     320      52

Cuts at:   3965    3992    4417    5081    6106    6645 
   Size:         27     425     664    1025     539

  Fragments arranged by size: 

                1480    1025     950     664     539     523     425     383
                 320     227      52      30      27
 
NaeI GCC'GGC

Cuts at:      0    4744    4944    6645 
   Size:       4744     200    1701

  Fragments arranged by size: 

                4744    1701     200
 
NarI GG'CG_CC

Cuts at:      0    2653    6645 
   Size:       2653    3992
 
NcoI C'CATG_G

Cuts at:      0    1323    1520    5595    6264    6645 
   Size:       1323     197    4075     669     381

  Fragments arranged by size: 

                4075    1323     669     381     197
 
NdeI CA'TA_TG

Cuts at:      0    1077    6645 
   Size:       1077    5568
 
NgoMIV G'CCGG_C

Cuts at:      0    4742    4942    6645 
   Size:       4742     200    1703

  Fragments arranged by size: 

                4742    1703     200
 
NsiI A_TGCA'T

Cuts at:      0    3672    6645 
   Size:       3672    2973
 
NspI r_CATG'y

Cuts at:      0    1042    1408    2086    2338    2917    3163    5550    6645 
   Size:       1042     366     678     252     579     246    2387    1095

  Fragments arranged by size: 

                2387    1095    1042     678     579     366     252     246
            
 
PasI CC'CwG_GG

Cuts at:      0     840    6645 
   Size:        840    5805
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3549    4246    4493    4696    6265    6645 
   Size:       3549     697     247     203    1569     380

  Fragments arranged by size: 

                3549    1569     697     380     247     203
 
PfoI T'CCnGG_A

Cuts at:      0    1593    4031    4353    4669    6182    6645 
   Size:       1593    2438     322     316    1513     463

  Fragments arranged by size: 

                2438    1593    1513     463     322     316
 
PmlI CAC'GTG

Cuts at:      0    4493    6645 
   Size:       4493    2152
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3523    3555    6645 
   Size:       3523      32    3090

  Fragments arranged by size: 

                3523    3090      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3596    4320    5424    6132    6645 
   Size:       3596     724    1104     708     513

  Fragments arranged by size: 

                3596    1104     724     708     513
 
PspOMI G'GGCC_C

Cuts at:      0    4501    6346    6645 
   Size:       4501    1845     299

  Fragments arranged by size: 

                4501    1845     299
 
PstI C_TGCA'G

Cuts at:      0     591    1479    4541    5080    5581    6146    6367    6645 
   Size:        591     888    3062     539     501     565     221     278

  Fragments arranged by size: 

                3062     888     591     565     539     501     278     221
            
 
PvuII CAG'CTG

Cuts at:      0    1207    1730    3210    3913    3992    5081    6645 
   Size:       1207     523    1480     703      79    1089    1564

  Fragments arranged by size: 

                1564    1480    1207    1089     703     523      79
 
SacI G_AGCT'C

Cuts at:      0    3989    4044    6645 
   Size:       3989      55    2601

  Fragments arranged by size: 

                3989    2601      55
 
SacII CC_GC'GG

Cuts at:      0     228    6645 
   Size:        228    6417
 
SalI G'TCGA_C

Cuts at:      0     672    1706    3190    6645 
   Size:        672    1034    1484    3455

  Fragments arranged by size: 

                3455    1484    1034     672
 
SanDI GG'GwC_CC

Cuts at:      0    3596    6645 
   Size:       3596    3049
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3220    6645 
   Size:       3220    3425
 
ScaI AGT'ACT

Cuts at:      0    1140    6645 
   Size:       1140    5505
 
SexAI A'CCwGG_T

Cuts at:      0    2225    4916    6645 
   Size:       2225    2691    1729

  Fragments arranged by size: 

                2691    2225    1729
 
SfcI C'TryA_G

Cuts at:      0     587     597     825    1475    1491    2123    2252    4537 
   Size:        587      10     228     650      16     632     129    2285

Cuts at:   4537    5076    5382    5577    5737    6142    6363    6645 
   Size:        539     306     195     160     405     221     282

  Fragments arranged by size: 

                2285     650     632     587     539     405     306     282
                 228     221     195     160     129      16      10
 
SfoI GGC'GCC

Cuts at:      0    2654    6645 
   Size:       2654    3991
 
SmaI CCC'GGG

Cuts at:      0    6338    6645 
   Size:       6338     307
 
SmlI C'TyrA_G

Cuts at:      0     304    2491    4043    6645 
   Size:        304    2187    1552    2602

  Fragments arranged by size: 

                2602    2187    1552     304
 
SpeI A'CTAG_T

Cuts at:      0    1503    4056    6645 
   Size:       1503    2553    2589

  Fragments arranged by size: 

                2589    2553    1503
 
SphI G_CATG'C

Cuts at:      0    2917    5550    6645 
   Size:       2917    2633    1095

  Fragments arranged by size: 

                2917    2633    1095
 
SspI AAT'ATT

Cuts at:      0    1194    3390    6645 
   Size:       1194    2196    3255

  Fragments arranged by size: 

                3255    2196    1194
 
StuI AGG'CCT

Cuts at:      0     571     760    3866    4346    6645 
   Size:        571     189    3106     480    2299

  Fragments arranged by size: 

                3106    2299     571     480     189
 
StyI C'CwwG_G

Cuts at:      0     755    1323    1520    1666    2037    2346    3648    4481 
   Size:        755     568     197     146     371     309    1302     833

Cuts at:   4481    5316    5595    5765    5999    6264    6645 
   Size:        835     279     170     234     265     381

  Fragments arranged by size: 

                1302     835     833     755     568     381     371     309
                 279     265     234     197     170     146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2898    4365    4849    6298    6422    6645 
   Size:       2898    1467     484    1449     124     223

  Fragments arranged by size: 

                2898    1467    1449     484     223     124
 
TatI w'GTAC_w

Cuts at:      0    1138    2507    3038    3336    4010    4100    5195    6645 
   Size:       1138    1369     531     298     674      90    1095    1450

  Fragments arranged by size: 

                1450    1369    1138    1095     674     531     298      90
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1241    1273    2294    2326    2588    2620    3429    3461 
   Size:       1241      32    1021      32     262      32     809      32

Cuts at:   3461    4592    4624    5194    5226    6645 
   Size:       1131      32     570      32    1419

  Fragments arranged by size: 

                1419    1241    1131    1021     809     570     262      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2851    3168    6645 
   Size:       2851     317    3477

  Fragments arranged by size: 

                3477    2851     317
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    6438    6645 
   Size:       6438     207
 
XmaI C'CCGG_G

Cuts at:      0    6336    6645 
   Size:       6336     309
 
XmnI GAAnn'nnTTC

Cuts at:      0      59    1224    5113    5434    6645 
   Size:         59    1165    3889     321    1211

  Fragments arranged by size: 

                3889    1211    1165     321      59
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfcI     SfoI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AloI     AscI     AseI
    AsiSI     BaeI     BglI     BmtI    BsiWI   BssHII    BssSI    BstBI
  BstZ17I    BtgZI   DraIII    EcoNI     FseI    FspAI     HpaI     MfeI
     MluI     NheI     NotI     NruI     PacI     PciI     PmeI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SbfI     SfiI     SgfI
    SgrAI    SnaBI     SrfI     SwaI     XbaI     XhoI     ZraI
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