################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccI AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp700I AspEI AvaI AviII AvrII BamHI BbsI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsiWI BsmBI BsoBI BspDI BspLU11I BsrDI BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRI EcoRV FseI FspI HincII HindII HindIII HpaI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SalI SapI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AatII 313 GACGTC GACGT^C Acc65I 587 GGTACC G^GTACC AccIII 470 TCCGGA T^CCGGA AcsI 1038 GAATTT [GA]^AATT[CT] AlwNI 1528 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1038 GAATTT [GA]^AATT[CT] AseI 1114 ATTAAT AT^TAAT AsnI 1114 ATTAAT AT^TAAT Asp718I 587 GGTACC G^GTACC AspI 2065 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 15 GGCGCC GGCGC^C BbrPI 1383 CACGTG CAC^GTG BbvCI 279 CCTCAGC CCTCAGC(-5/-2) BclI 1994 TGATCA T^GATCA BfaI 86 CTAG C^TAG BsaAI 1383 CACGTG [CT]AC^GT[GA] BsaBI 1174 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 70 GGTCTC GGTCTC(1/5) BseAI 470 TCCGGA T^CCGGA BsmI 1717 GAATGC GAATGC(1/-1) Bsp1407I 1031 TGTACA T^GTACA BspEI 470 TCCGGA T^CCGGA BspHI 2072 TCATGA T^CATGA BsrBI 1434 GAGCGG CCGCTC(-3/-3) BsrFI 1335 GCCGGT [GA]^CCGG[CT] BsrGI 1031 TGTACA T^GTACA BssHII 47 GCGCGC G^CGCGC BstEII 1368 GGTCACC G^GT[ACGT]ACC BstPI 1368 GGTCACC G^GT[ACGT]ACC Cfr10I 1335 GCCGGT [GA]^CCGG[CT] EagI 1333 CGGCCG C^GGCCG EarI 1183 CTCTTC CTCTTC(1/4) EclXI 1333 CGGCCG C^GGCCG Eco52I 1333 CGGCCG C^GGCCG EcoO65I 1368 GGTCACC G^GT[ACGT]ACC FbaI 1994 TGATCA T^GATCA KasI 15 GGCGCC G^GCGCC KpnI 587 GGTACC GGTAC^C Ksp632I 1183 CTCTTC CTCTTC(1/4) KspI 4 CCGCGG CCGC^GG MaeI 86 CTAG C^TAG MamI 1174 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 470 TCCGGA T^CCGGA NarI 15 GGCGCC GG^CGCC NcoI 1695 CCATGG C^CATGG NdeI 78 CATATG CA^TATG PflFI 2065 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1383 CACGTG CAC^GTG PmlI 1383 CACGTG CAC^GTG PpuMI 1438 GGGACCT [GA]G^G[AT]CC[CT] PshAI 1440 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1114 ATTAAT AT^TAAT PvuII 1584 CAGCTG CAG^CTG RcaI 2072 TCATGA T^CATGA SacI 2092 GAGCTC GAGCT^C SacII 4 CCGCGG CCGC^GG ScaI 756 AGTACT AGT^ACT SexAI 1486 ACCAGGT A^CC[AT]GGT SfoI 15 GGCGCC GGC^GCC SpeI 85 ACTAGT A^CTAGT SphI 1900 GCATGC GCATG^C SspBI 1031 TGTACA T^GTACA SspI 1096 AATATT AAT^ATT StuI 870 AGGCCT AGG^CCT TfiI 1343 GATTC G^A[AT]TC Tth111I 2065 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 15 GGCGCC G[GA]^CG[CT]C 313 GACGTC ApaI 8 GGGCCC GGGCC^C 2288 GGGCCC BalI 891 TGGCCA TGG^CCA 1794 TGGCCA BcgI 918 CGAGTACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 482 GCACCAAACTCG BciVI 1130 GTATCC GTATCC(6/5) 1213 GTATCC BglII 1625 AGATCT A^GATCT 1799 AGATCT BpmI 760 CTCCAG CTGGAG(16/14) 1357 CTGGAG BsaHI 15 GGCGCC G[GA]^CG[CT]C 313 GACGTC BseRI 267 GAGGAG GAGGAG(10/8) 496 GAGGAG BsiEI 1333 CGGCCG CG[GA][CT]^CG 2247 CGACCG BstAPI 1484 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1589 GCAACCAGTGC BstXI 932 CCAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2165 CCATCCTCCTGG DraIII 1485 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2341 CACACTGTG EcoNI 903 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1661 CCTGTGCCAGG EcoT14I 1695 CCATGG C^C[AT][AT]GG 2060 CCAAGG EcoT22I 1118 ATGCAT ATGCA^T 1898 ATGCAT HaeII 15 GGCGCC [GA]GCGC^[CT] 1417 AGCGCC Hin1I 15 GGCGCC G[GA]^CG[CT]C 313 GACGTC MaeII 314 ACGT A^CGT 1384 ACGT MluNI 891 TGGCCA TGG^CCA 1794 TGGCCA MscI 891 TGGCCA TGG^CCA 1794 TGGCCA MseI 1115 TTAA T^TAA 1178 TTAA NsiI 1118 ATGCAT ATGCA^T 1898 ATGCAT PflMI 883 CCACACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2134 CCAGAGTGTGG PleI 1087 GACTC GAGTC(4/5) 1679 GAGTC StyI 1695 CCATGG C^C[AT][AT]GG 2060 CCAAGG Tru9I 1115 TTAA T^TAA 1178 TTAA Van91I 883 CCACACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2134 CCAGAGTGTGG XcmI 130 CCAAAATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2336 CCACGCACACTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AocI AspHI AvaII BanI BanII BbvI BcnI BmyI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII