Restriction map for pF1KB5741
 (Linear) (Six-base) MAPSORT of: KIBB5741.seq  Check: 6801  from: 1  to: 2397



KIBB5741.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1897    2397 
   Size:       1897     500
 
AatII G_ACGT'C

Cuts at:      0     317    2397 
   Size:        317    2080
 
Acc65I G'GTAC_C

Cuts at:      0     587    2397 
   Size:        587    1810
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1267    2397 
   Size:       1267    1130
 
AleI CACnn'nnGTG

Cuts at:      0    2002    2014    2086    2397 
   Size:       2002      12      72     311

  Fragments arranged by size: 

                2002     311      72      12
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     789    1340    2397 
   Size:        789     551    1057

  Fragments arranged by size: 

                1057     789     551
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1533    2397 
   Size:       1533     864
 
ApaI G_GGCC'C

Cuts at:      0      12    2292    2397 
   Size:         12    2280     105

  Fragments arranged by size: 

                2280     105      12
 
ApoI r'AATT_y

Cuts at:      0    1038    2397 
   Size:       1038    1359
 
AseI AT'TA_AT

Cuts at:      0    1115    2397 
   Size:       1115    1282
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     571     604    1113    1146    2397 
   Size:        571      33     509      33    1251

  Fragments arranged by size: 

                1251     571     509      33      33
 
BanI G'GyrC_C

Cuts at:      0      15     481     579     587    1483    2397 
   Size:         15     466      98       8     896     914

  Fragments arranged by size: 

                 914     896     466      98      15       8
 
BanII G_rGCy'C

Cuts at:      0      12      44     244     301    2096    2292    2397 
   Size:         12      32     200      57    1795     196     105

  Fragments arranged by size: 

                1795     200     196     105      57      32      12
 
BbeI G_GCGC'C

Cuts at:      0      19    2397 
   Size:         19    2378
 
BbvCI CC'TCA_GC

Cuts at:      0     280    2397 
   Size:        280    2117
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     471     505     907     941    2397 
   Size:        471      34     402      34    1456

  Fragments arranged by size: 

                1456     471     402      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1141    1224    2397 
   Size:       1141      83    1173

  Fragments arranged by size: 

                1173    1141      83
 
BclI T'GATC_A

Cuts at:      0    1994    2397 
   Size:       1994     403
 
BfrBI ATG'CAT

Cuts at:      0    1120    1900    2397 
   Size:       1120     780     497

  Fragments arranged by size: 

                1120     780     497
 
BglII A'GATC_T

Cuts at:      0    1625    1799    2397 
   Size:       1625     174     598

  Fragments arranged by size: 

                1625     598     174
 
Bme1580I G_kGCm'C

Cuts at:      0      12     484     842    1486    1820    1885    2292    2397 
   Size:         12     472     358     644     334      65     407     105

  Fragments arranged by size: 

                 644     472     407     358     334     105      65      12
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2093    2397 
   Size:       2093     304
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745    1378    2397 
   Size:        745     633    1019

  Fragments arranged by size: 

                1019     745     633
 
Bpu10I CC'TnA_GC

Cuts at:      0     280    1689    2397 
   Size:        280    1409     708

  Fragments arranged by size: 

                1409     708     280
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      76    2397 
   Size:         76    2321
 
BsaAI yAC'GTr

Cuts at:      0    1385    2397 
   Size:       1385    1012
 
BsaBI GATnn'nnATC

Cuts at:      0    1178    2397 
   Size:       1178    1219
 
BsaHI Gr'CG_yC

Cuts at:      0      16     314    2397 
   Size:         16     298    2083

  Fragments arranged by size: 

                2083     298      16
 
BsaWI w'CCGG_w

Cuts at:      0     470     524    1133    2249    2397 
   Size:        470      54     609    1116     148

  Fragments arranged by size: 

                1116     609     470     148      54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     860     890    1306    1336    1582    1612    1642    1672 
   Size:        860      30     416      30     246      30      30      30

Cuts at:   1672    2122    2152    2397 
   Size:        450      30     245

  Fragments arranged by size: 

                 860     450     416     246     245      30      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     282     511    2397 
   Size:        282     229    1886

  Fragments arranged by size: 

                1886     282     229
 
BseYI C'CCAG_C

Cuts at:      0     243     308    1300    2397 
   Size:        243      65     992    1097

  Fragments arranged by size: 

                1097     992     243      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     898    1365    1485    1584    2397 
   Size:        898     467     120      99     813

  Fragments arranged by size: 

                 898     813     467     120      99
 
BsiEI CG_ry'CG

Cuts at:      0    1336    2250    2397 
   Size:       1336     914     147

  Fragments arranged by size: 

                1336     914     147
 
BsiHKAI G_wGCw'C

Cuts at:      0     148    1600    2096    2397 
   Size:        148    1452     496     301

  Fragments arranged by size: 

                1452     496     301     148
 
BsmI GAATG_Cn'

Cuts at:      0    1723    2397 
   Size:       1723     674
 
Bsp1286I G_dGCh'C

Cuts at:      0      12      44     148     244     301     484     842    1486 
   Size:         12      32     104      96      57     183     358     644

Cuts at:   1486    1600    1820    1885    2096    2292    2397 
   Size:        114     220      65     211     196     105

  Fragments arranged by size: 

                 644     358     220     211     196     183     114     105
                 104      96      65      57      32      12
 
BspEI T'CCGG_A

Cuts at:      0     470    2397 
   Size:        470    1927
 
BspHI T'CATG_A

Cuts at:      0    2072    2397 
   Size:       2072     325
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      58    1207    1723    1897    1975    2397 
   Size:         58    1149     516     174      78     422

  Fragments arranged by size: 

                1149     516     422     174      78      58
 
BsrBI CCG'CTC

Cuts at:      0    1436    2397 
   Size:       1436     961
 
BsrFI r'CCGG_y

Cuts at:      0    1335    2397 
   Size:       1335    1062
 
BsrGI T'GTAC_A

Cuts at:      0    1031    2397 
   Size:       1031    1366
 
BssHII G'CGCG_C

Cuts at:      0      47    2397 
   Size:         47    2350
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1490    1595    2397 
   Size:       1490     105     802

  Fragments arranged by size: 

                1490     802     105
 
BstEII G'GTnAC_C

Cuts at:      0    1368    2397 
   Size:       1368    1029
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     939    2172    2397 
   Size:        939    1233     225

  Fragments arranged by size: 

                1233     939     225
 
BstYI r'GATC_y

Cuts at:      0    1173    1625    1799    2397 
   Size:       1173     452     174     598

  Fragments arranged by size: 

                1173     598     452     174
 
Bsu36I CC'TnA_GG

Cuts at:      0     265     737    1169    2125    2397 
   Size:        265     472     432     956     272

  Fragments arranged by size: 

                 956     472     432     272     265
 
BtgI C'CryG_G

Cuts at:      0       4     169    1695    2397 
   Size:          4     165    1526     702

  Fragments arranged by size: 

                1526     702     165       4
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     831    2397 
   Size:        831    1566
 
BtsI GCAGTG_nn'

Cuts at:      0     805     923    1013    1707    1840    2397 
   Size:        805     118      90     694     133     557

  Fragments arranged by size: 

                 805     694     557     133     118      90
 
DraIII CAC_nnn'GTG

Cuts at:      0    1490    2346    2397 
   Size:       1490     856      51

  Fragments arranged by size: 

                1490     856      51
 
EaeI y'GGCC_r

Cuts at:      0     891    1333    1794    2397 
   Size:        891     442     461     603

  Fragments arranged by size: 

                 891     603     461     442
 
EagI C'GGCC_G

Cuts at:      0    1333    2397 
   Size:       1333    1064
 
EarI CTCTTCn'nnn_

Cuts at:      0    1189    2397 
   Size:       1189    1208
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     640    2397 
   Size:        640    1757
 
EcoICRI GAG'CTC

Cuts at:      0    2094    2397 
   Size:       2094     303
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745    1267    1378    2397 
   Size:        745     522     111    1019

  Fragments arranged by size: 

                1019     745     522     111
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     907    1665    2397 
   Size:        907     758     732

  Fragments arranged by size: 

                 907     758     732
 
EcoO109I rG'GnC_Cy

Cuts at:      0       9    1439    1856    2288    2397 
   Size:          9    1430     417     432     109

  Fragments arranged by size: 

                1430     432     417     109       9
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     390     474    2397 
   Size:        390      84    1923

  Fragments arranged by size: 

                1923     390      84
 
HaeII r_GCGC'y

Cuts at:      0      19    1421    2397 
   Size:         19    1402     976

  Fragments arranged by size: 

                1402     976      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     930     934     962     966    1072    1104    1198    1230 
   Size:        930       4      28       4     106      32      94      32

Cuts at:   1230    1249    1281    1642    1674    1907    1939    2397 
   Size:         19      32     361      32     233      32     458

  Fragments arranged by size: 

                 930     458     361     233     106      94      32      32
                  32      32      32      28      19       4       4
 
KasI G'GCGC_C

Cuts at:      0      15    2397 
   Size:         15    2382
 
KpnI G_GTAC'C

Cuts at:      0     591    2397 
   Size:        591    1806
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     956    2135    2397 
   Size:        956    1179     262

  Fragments arranged by size: 

                1179     956     262
 
MscI TGG'CCA

Cuts at:      0     893    1796    2397 
   Size:        893     903     601

  Fragments arranged by size: 

                 903     893     601
 
MslI CAynn'nnrTG

Cuts at:      0     322    2002    2014    2086    2397 
   Size:        322    1680      12      72     311

  Fragments arranged by size: 

                1680     322     311      72      12
 
MspA1I CmG'CkG

Cuts at:      0       6      21    1568    1586    2397 
   Size:          6      15    1547      18     811

  Fragments arranged by size: 

                1547     811      18      15       6
 
NarI GG'CG_CC

Cuts at:      0      16    2397 
   Size:         16    2381
 
NcoI C'CATG_G

Cuts at:      0    1695    2397 
   Size:       1695     702
 
NdeI CA'TA_TG

Cuts at:      0      79    2397 
   Size:         79    2318
 
NsiI A_TGCA'T

Cuts at:      0    1122    1902    2397 
   Size:       1122     780     495

  Fragments arranged by size: 

                1122     780     495
 
NspI r_CATG'y

Cuts at:      0    1321    1781    1904    2397 
   Size:       1321     460     123     493

  Fragments arranged by size: 

                1321     493     460     123
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     889    2140    2397 
   Size:        889    1251     257

  Fragments arranged by size: 

                1251     889     257
 
PfoI T'CCnGG_A

Cuts at:      0     718    1346    2397 
   Size:        718     628    1051

  Fragments arranged by size: 

                1051     718     628
 
PmlI CAC'GTG

Cuts at:      0    1385    2397 
   Size:       1385    1012
 
PpuMI rG'GwC_Cy

Cuts at:      0    1439    2397 
   Size:       1439     958
 
PshAI GACnn'nnGTC

Cuts at:      0    1444    2397 
   Size:       1444     953
 
PspOMI G'GGCC_C

Cuts at:      0       8    2288    2397 
   Size:          8    2280     109

  Fragments arranged by size: 

                2280     109       8
 
PstI C_TGCA'G

Cuts at:      0     306    1396    1585    1807    2397 
   Size:        306    1090     189     222     590

  Fragments arranged by size: 

                1090     590     306     222     189
 
PvuII CAG'CTG

Cuts at:      0    1586    2397 
   Size:       1586     811
 
SacI G_AGCT'C

Cuts at:      0    2096    2397 
   Size:       2096     301
 
SacII CC_GC'GG

Cuts at:      0       7    2397 
   Size:          7    2390
 
ScaI AGT'ACT

Cuts at:      0     758    2397 
   Size:        758    1639
 
SexAI A'CCwGG_T

Cuts at:      0    1486    2397 
   Size:       1486     911
 
SfcI C'TryA_G

Cuts at:      0     302     382    1392    1581    1803    2397 
   Size:        302      80    1010     189     222     594

  Fragments arranged by size: 

                1010     594     302     222     189      80
 
SfoI GGC'GCC

Cuts at:      0      17    2397 
   Size:         17    2380
 
SpeI A'CTAG_T

Cuts at:      0      85    2397 
   Size:         85    2312
 
SphI G_CATG'C

Cuts at:      0    1904    2397 
   Size:       1904     493
 
SspI AAT'ATT

Cuts at:      0    1098    2397 
   Size:       1098    1299
 
StuI AGG'CCT

Cuts at:      0     872    2397 
   Size:        872    1525
 
StyI C'CwwG_G

Cuts at:      0    1695    2060    2397 
   Size:       1695     365     337

  Fragments arranged by size: 

                1695     365     337
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     143    1992    2397 
   Size:        143    1849     405

  Fragments arranged by size: 

                1849     405     143
 
TatI w'GTAC_w

Cuts at:      0     419     756     920    1031    2397 
   Size:        419     337     164     111    1366

  Fragments arranged by size: 

                1366     419     337     164     111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     860     892    1052    1084    1708    1740    2122    2154 
   Size:        860      32     160      32     624      32     382      32

Cuts at:   2154    2397 
   Size:        243

  Fragments arranged by size: 

                 860     624     382     243     160      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2068    2397 
   Size:       2068     329
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     137    2343    2397 
   Size:        137    2206      54

  Fragments arranged by size: 

                2206     137      54
 
ZraI GAC'GTC

Cuts at:      0     315    2397 
   Size:        315    2082
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AleI     AlfI    AlwNI     ApaI
     ApoI     AseI     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NsiI     NspI    PflMI     PfoI     PmlI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI    SacII     ScaI    SexAI     SfcI
     SfoI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AloI
    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BbsI     BglI
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsiWI    BsmBI    BsrDI
    BssSI    BstBI  BstZ17I     ClaI    CspCI     DraI     DrdI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI
     MluI     NaeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII
     SalI    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SrfI     SwaI     XbaI     XhoI     XmaI     XmnI
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