Restriction map for pF1KE0025
 (Linear) (Six-base) MAPSORT of: KIEE0025.seq  Check: 9575  from: 1  to: 1722



KIEE0025.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

ApoI r'AATT_y

Cuts at:      0     493    1062    1088    1722 
   Size:        493     569      26     634

  Fragments arranged by size: 

                 634     569     493      26
 
AvaI C'yCGr_G

Cuts at:      0     160     277     984    1025    1631    1722 
   Size:        160     117     707      41     606      91

  Fragments arranged by size: 

                 707     606     160     117      91      41
 
BanI G'GyrC_C

Cuts at:      0     164    1722 
   Size:        164    1558
 
BanII G_rGCy'C

Cuts at:      0     722    1638    1722 
   Size:        722     916      84

  Fragments arranged by size: 

                 916     722      84
 
BbeI G_GCGC'C

Cuts at:      0     168    1722 
   Size:        168    1554
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1486    1520    1722 
   Size:       1486      34     202

  Fragments arranged by size: 

                1486     202      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1321    1722 
   Size:       1321     401
 
BclI T'GATC_A

Cuts at:      0     594    1107    1722 
   Size:        594     513     615

  Fragments arranged by size: 

                 615     594     513
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     177    1374    1722 
   Size:        177    1197     348

  Fragments arranged by size: 

                1197     348     177
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1657    1722 
   Size:       1657      65
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     830    1722 
   Size:        830     892
 
Bpu10I CC'TnA_GC

Cuts at:      0    1409    1722 
   Size:       1409     313
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     160     891    1722 
   Size:        160     731     831

  Fragments arranged by size: 

                 831     731     160
 
BsaHI Gr'CG_yC

Cuts at:      0     165    1722 
   Size:        165    1557
 
BsaWI w'CCGG_w

Cuts at:      0     698    1722 
   Size:        698    1024
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100     380     410     533     563    1722 
   Size:         70      30     280      30     123      30    1159

  Fragments arranged by size: 

                1159     280     123      70      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148    1061    1722 
   Size:        148     913     661

  Fragments arranged by size: 

                 913     661     148
 
BseYI C'CCAG_C

Cuts at:      0     111    1722 
   Size:        111    1611
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673    1722 
   Size:        673    1049
 
BsiHKAI G_wGCw'C

Cuts at:      0     125    1722 
   Size:        125    1597
 
BsmI GAATG_Cn'

Cuts at:      0     942    1722 
   Size:        942     780
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1214    1722 
   Size:       1214     508
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     722    1638    1722 
   Size:        125     597     916      84

  Fragments arranged by size: 

                 916     597     125      84
 
BspEI T'CCGG_A

Cuts at:      0     698    1722 
   Size:        698    1024
 
BspHI T'CATG_A

Cuts at:      0     591     689    1722 
   Size:        591      98    1033

  Fragments arranged by size: 

                1033     591      98
 
BsrBI CCG'CTC

Cuts at:      0     176    1722 
   Size:        176    1546
 
BsrDI GCAATG_nn'

Cuts at:      0      17    1722 
   Size:         17    1705
 
BsrGI T'GTAC_A

Cuts at:      0     343    1661    1722 
   Size:        343    1318      61

  Fragments arranged by size: 

                1318     343      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     961    1585    1722 
   Size:        961     624     137

  Fragments arranged by size: 

                 961     624     137
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1473    1508    1722 
   Size:       1473      35     214

  Fragments arranged by size: 

                1473     214      35
 
DraI TTT'AAA

Cuts at:      0     951    1275    1722 
   Size:        951     324     447

  Fragments arranged by size: 

                 951     447     324
 
EaeI y'GGCC_r

Cuts at:      0      68    1722 
   Size:         68    1654
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     199     657    1722 
   Size:        130      69     458    1065

  Fragments arranged by size: 

                1065     458     130      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     830    1722 
   Size:        830     892
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1408    1722 
   Size:       1408     314
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81    1510    1722 
   Size:         81    1429     212

  Fragments arranged by size: 

                1429     212      81
 
EcoRI G'AATT_C

Cuts at:      0     493    1722 
   Size:        493    1229
 
EcoRV GAT'ATC

Cuts at:      0     816    1722 
   Size:        816     906
 
HaeII r_GCGC'y

Cuts at:      0     168    1722 
   Size:        168    1554
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225     579     611    1722 
   Size:        193      32     354      32    1111

  Fragments arranged by size: 

                1111     354     193      32      32
 
HincII GTy'rAC

Cuts at:      0    1038    1722 
   Size:       1038     684
 
HindIII A'AGCT_T

Cuts at:      0     321    1170    1722 
   Size:        321     849     552

  Fragments arranged by size: 

                 849     552     321
 
KasI G'GCGC_C

Cuts at:      0     164    1722 
   Size:        164    1558
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     468    1722 
   Size:        468    1254
 
MscI TGG'CCA

Cuts at:      0      70    1722 
   Size:         70    1652
 
MspA1I CmG'CkG

Cuts at:      0     134     337     418    1722 
   Size:        134     203      81    1304

  Fragments arranged by size: 

                1304     203     134      81
 
NarI GG'CG_CC

Cuts at:      0     165    1722 
   Size:        165    1557
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1249    1722 
   Size:       1249     473
 
PpuMI rG'GwC_Cy

Cuts at:      0      81    1510    1722 
   Size:         81    1429     212

  Fragments arranged by size: 

                1429     212      81
 
PshAI GACnn'nnGTC

Cuts at:      0     533    1722 
   Size:        533    1189
 
PsiI TTA'TAA

Cuts at:      0      41    1722 
   Size:         41    1681
 
PspXI vC'TCGA_Gb

Cuts at:      0     277    1025    1631    1722 
   Size:        277     748     606      91

  Fragments arranged by size: 

                 748     606     277      91
 
PstI C_TGCA'G

Cuts at:      0     503    1722 
   Size:        503    1219
 
PvuII CAG'CTG

Cuts at:      0     337     418    1722 
   Size:        337      81    1304

  Fragments arranged by size: 

                1304     337      81
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130     657    1722 
   Size:        130     527    1065

  Fragments arranged by size: 

                1065     527     130
 
ScaI AGT'ACT

Cuts at:      0    1031    1722 
   Size:       1031     691
 
SfcI C'TryA_G

Cuts at:      0     499    1722 
   Size:        499    1223
 
SfoI GGC'GCC

Cuts at:      0     166    1722 
   Size:        166    1556
 
SmlI C'TyrA_G

Cuts at:      0     277    1025    1631    1722 
   Size:        277     748     606      91

  Fragments arranged by size: 

                 748     606     277      91
 
SspI AAT'ATT

Cuts at:      0     309    1682    1722 
   Size:        309    1373      40

  Fragments arranged by size: 

                1373     309      40
 
StuI AGG'CCT

Cuts at:      0     215    1722 
   Size:        215    1507
 
StyI C'CwwG_G

Cuts at:      0     531    1057    1615    1722 
   Size:        531     526     558     107

  Fragments arranged by size: 

                 558     531     526     107
 
TatI w'GTAC_w

Cuts at:      0     343     791    1029    1661    1722 
   Size:        343     448     238     632      61

  Fragments arranged by size: 

                 632     448     343     238      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     380     412     428     460     533     565    1722 
   Size:        380      32      16      32      73      32    1157

  Fragments arranged by size: 

                1157     380      73      32      32      32      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     574    1722 
   Size:        574    1148
 
XhoI C'TCGA_G

Cuts at:      0     277    1025    1631    1722 
   Size:        277     748     606      91

  Fragments arranged by size: 

                 748     606     277      91
 

 Enzymes that do cut:

     ApoI     AvaI     BanI    BanII     BbeI     BcgI    BciVI     BclI
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BstAPI    CspCI     DraI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI   MspA1I     NarI    PflMI
    PpuMI    PshAI     PsiI    PspXI     PstI    PvuII     SapI     ScaI
     SfcI     SfoI     SmlI     SspI     StuI     StyI     TatI     TstI
     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BbvCI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
    BpuEI    BsaAI    BsaBI    BsiEI    BsiWI    BspMI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI
     FalI     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII  Tth111I     XbaI     XmaI
     XmnI     ZraI
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