Restriction map for pF1KE1011
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI           56  CCGC                 CCGC(-3/-1)
AflIII        656  ACATGT               A^C[GA][CT]GT
AvaI          600  CTCGGG               C^[CT]CG[GA]G
BanI          145  GGCACC               G^G[CT][GA]CC
BcnI          437  CCCGG                CC^[GC]GG
BpmI          564  CTCCAG               CTGGAG(16/14)
BsoBI         600  CTCGGG               C^[CT]CG[GA]G
BspLU11I      656  ACATGT               A^CATGT
BspMI          51  ACCTGC               ACCTGC(4/8)
BsrI          566  CCAGT                ACTGG(1/-1)
BstAPI        122  GCAGCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          29  CCAGG                CC^[AT]GG
BstYI         357  AGATCC               [GA]^GATC[CT]
Cfr13I        434  GGCCC                G^G[ACGT]CC
DraI          115  TTTAAA               TTT^AAA
DraII         433  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI            5  CCATGG               C^C[GA][CT]GG
EarI           18  GAAGAG               CTCTTC(1/4)
EcoO109I      433  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII         29  CCAGG                ^CC[AT]GG
EcoT14I         5  CCATGG               C^C[AT][AT]GG
HapII         438  CCGG                 C^CGG
HgaI           83  GCGTC                GACGC(5/10)
HpaII         438  CCGG                 C^CGG
HphI          497  TCACC                GGTGA(8/7)
Ksp632I        18  GAAGAG               CTCTTC(1/4)
MflI          357  AGATCC               [GA]^GATC[CT]
MspA1I        178  CAGCTG               C[AC]G^C[GT]G
MspI          438  CCGG                 C^CGG
MvaI           29  CCAGG                CC^[AT]GG
NciI          437  CCCGG                CC^[GC]GG
NcoI            5  CCATGG               C^CATGG
NspI          656  ACATGT               [GA]CATG^[CT]
PleI          528  GACTC                GAGTC(4/5)
PvuII         178  CAGCTG               CAG^CTG
SapI           18  GAAGAGC              GCTCTTC(1/4)
Sau96I        434  GGCCC                G^G[ACGT]CC
SfaNI         125  GCATC                GCATC(5/9)
SmlI          429  CTCAAG               C^T[CT][GA]AG
StyI            5  CCATGG               C^C[AT][AT]GG
TaqI          541  TCGA                 T^CGA
TfiI          676  GAATC                G^A[AT]TC
TthHB8I       541  TCGA                 T^CGA
XhoII         357  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AluI          179  AGCT                 AG^CT
              598  AGCT                
AlwI          358  GATCC                GGATC(4/5)
              167  GGATC               
AlwNI         123  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              479  CAGTAACTG           
AspHI         459  GAGCAC               G[AT]GC[AT]^C
              612  GAGCAC              
BanII          21  GAGCCC               G[GA]GC[CT]^C
              624  GGGCTC              
BbvI          122  GCAGC                GCAGC(8/12)
              596  GCAGC               
BfaI          299  CTAG                 C^TAG
              365  CTAG                
BseRI         534  CTCCTC               GAGGAG(10/8)
              627  CTCCTC              
BsiHKAI       459  GAGCAC               G[AT]GC[AT]^C
              612  GAGCAC              
BsiYI         225  CCCTAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              566  CCAGTGCAGGG         
BslI          225  CCCTAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              566  CCAGTGCAGGG         
BssKI          29  CCAGG                ^CC[ACGT]GG
              437  CCCGG               
BstF5I        545  CATCC                GGATG(2/0)
              551  CATCC               
DdeI          176  CTCAG                C^T[ACGT]AG
              275  CTGAG               
DpnII         168  GATC                 ^GATC
              358  GATC                
FokI          545  CATCC                GGATG(9/13)
              551  CATCC               
HaeIII         32  GGCC                 GG^CC
              434  GGCC                
HinfI         528  GACTC                G^A[ACGT]TC
              676  GAATC               
MaeI          299  CTAG                 C^TAG
              365  CTAG                
MaeII         449  ACGT                 A^CGT
              494  ACGT                
MboI          168  GATC                 ^GATC
              358  GATC                
MboII          18  GAAGA                GAAGA(8/7)
              237  GAAGA               
MseI          116  TTAA                 T^TAA
              518  TTAA                
MwoI           46  GCGAAACCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              122  GCAGCATCTGC         
NdeII         168  GATC                 ^GATC
              358  GATC                
NlaIII          6  CATG                 CATG^
              657  CATG                
Sau3AI        168  GATC                 ^GATC
              358  GATC                
ScrFI          29  CCAGG                CC^[ACGT]GG
              437  CCCGG               
Tru9I         116  TTAA                 T^TAA
              518  TTAA                
TseI          122  GCAGC                G^C[AT]GC
              596  GCAGC               
Tsp45I        496  GTCAC                ^GT[GC]AC
              559  GTGAC               
Tsp509I       172  AATT                 ^AATT
              291  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

BmyI        BsaJI       BsgI        Bsp1286I    Fnu4HI      
ItaI        MaeIII      MnlI        NlaIV       TspRI       
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