Restriction map for pF1KB5884
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AlwI        AlwNI       Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     AspI        
AviII       AvrII       BamHI       BbeI        BcgI        
BclI        BfaI        BfrI        BlnI        BsaBI       
BsaI        BseAI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eco47III    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I        555  GGTACC               G^GTACC
AccI         1000  GTAGAC               GT^[AC][GT]AC
AgeI          281  ACCGGT               A^CCGGT
Alw44I        304  GTGCAC               G^TGCAC
ApaLI         304  GTGCAC               G^TGCAC
Asp718I       555  GGTACC               G^GTACC
BalI          416  TGGCCA               TGG^CCA
BbsI          328  GTCTTC               GAAGAC(2/6)
BbvCI         201  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1214  GTATCC               GTATCC(6/5)
BglII         602  AGATCT               A^GATCT
BpuAI         328  GTCTTC               GAAGAC(2/6)
BsaWI         281  ACCGGT               [AT]^CCGG[AT]
BsiEI         651  CGGCCG               CG[GA][CT]^CG
BsmAI         847  GAGAC                GTCTC(1/5)
Bsp1407I     1393  TGTACA               T^GTACA
BspMI         686  ACCTGC               ACCTGC(4/8)
BsrDI         752  GCAATG               GCAATG(2/0)
BsrGI        1393  TGTACA               T^GTACA
BssHII        400  GCGCGC               G^CGCGC
BstAPI        599  GCAAGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         602  AGATCT               [GA]^GATC[CT]
EagI          651  CGGCCG               C^GGCCG
EclXI         651  CGGCCG               C^GGCCG
Eco52I        651  CGGCCG               C^GGCCG
KpnI          555  GGTACC               GGTAC^C
MaeIII        425  GTGAC                ^GT[ACGT]AC
MflI          602  AGATCT               [GA]^GATC[CT]
MluNI         416  TGGCCA               TGG^CCA
MscI          416  TGGCCA               TGG^CCA
MslI          342  CATCGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PinAI         281  ACCGGT               A^CCGGT
SacI            6  GAGCTC               GAGCT^C
SapI           69  GCTCTTC              GCTCTTC(1/4)
SspBI        1393  TGTACA               T^GTACA
TaqI           74  TCGA                 T^CGA
Tsp45I        425  GTGAC                ^GT[GC]AC
TthHB8I        74  TCGA                 T^CGA
XhoII         602  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          427  GACGCC               G[GA]^CG[CT]C
             1302  GGCGTC              
AhdI          916  GACAGTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACCTGGCGTC         
AspEI         916  GACAGTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACCTGGCGTC         
BbrPI         250  CACGTG               CAC^GTG
             1161  CACGTG              
BsaAI         250  CACGTG               [CT]AC^GT[GA]
             1161  CACGTG              
BsaHI         427  GACGCC               G[GA]^CG[CT]C
             1302  GGCGTC              
BsrBI          76  GAGCGG               CCGCTC(-3/-3)
             1356  GAGCGG              
BssSI         438  CTCGTG               CACGAG(-5/-1)
              676  CTCGTG              
BstXI         137  CCACCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1017  CCAGGTGCCTGG        
DraIII        523  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1180  CACCCAGTG           
Eam1105I      916  GACAGTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1297  GACCTGGCGTC         
EarI           70  CTCTTC               CTCTTC(1/4)
              334  CTCTTC              
EcoT14I        22  CCAAGG               C^C[AT][AT]GG
             1452  CCTTGG              
HgaI          427  GACGC                GACGC(5/10)
             1303  GCGTC               
Hin1I         427  GACGCC               G[GA]^CG[CT]C
             1302  GGCGTC              
HphI         1266  GGTGA                GGTGA(8/7)
              461  TCACC               
Ksp632I        70  CTCTTC               CTCTTC(1/4)
              334  CTCTTC              
PflMI         189  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1179  CCACCCAGTGG         
PmaCI         250  CACGTG               CAC^GTG
             1161  CACGTG              
PmlI          250  CACGTG               CAC^GTG
             1161  CACGTG              
PstI           11  CTGCAG               CTGCA^G
             1045  CTGCAG              
SfaNI         310  GATGC                GCATC(5/9)
              872  GCATC               
StuI          672  AGGCCT               AGG^CCT
             1084  AGGCCT              
StyI           22  CCAAGG               C^C[AT][AT]GG
             1452  CCTTGG              
Van91I        189  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1179  CCACCCAGTGG         
XcmI          500  CCACATTCGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1017  CCAGGTGCCTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AocI        
ApaI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco81I      
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        SmaI        TseI        TspRI       
XmaCI       XmaI        
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