Restriction map for pF1KE0009
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AviII       BbeI        BbrPI       BbvCI       
BclI        BfrI        BglI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstZ17I     CelII       Cfr10I      CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       KasI        KpnI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Van91I      XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AlwNI        1874  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          526  CCTCAGG              CC^T[ACGT]AGG
AspI         1729  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII          76  CCTAGG               C^CTAGG
BalI         1662  TGGCCA               TGG^CCA
BamHI        1643  GGATCC               G^GATCC
BanI          425  GGTGCC               G^G[CT][GA]CC
BbsI         1379  GTCTTC               GAAGAC(2/6)
BcgI          989  GCAATTCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1811  GGATAC               GTATCC(6/5)
BglII          95  AGATCT               A^GATCT
BlnI           76  CCTAGG               C^CTAGG
BpuAI        1379  GTCTTC               GAAGAC(2/6)
BsaI          619  GAGACC               GGTCTC(1/5)
Bsp1407I      329  TGTACA               T^GTACA
BspDI        1219  ATCGAT               AT^CGAT
BspMI         367  ACCTGC               ACCTGC(4/8)
BsrGI         329  TGTACA               T^GTACA
BstAPI        362  GCACTACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1254  GGTCACC              G^GT[ACGT]ACC
BstPI        1254  GGTCACC              G^GT[ACGT]ACC
Bsu36I        526  CCTCAGG              CC^T[ACGT]AGG
CfoI          876  GCGC                 GCG^C
ClaI         1219  ATCGAT               AT^CGAT
DraII         803  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI         1662  TGGCCA               [CT]^GGCC[GA]
Eco81I        526  CCTCAGG              CC^T[ACGT]AGG
EcoNI         859  CCTCCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      803  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1254  GGTCACC              G^GT[ACGT]ACC
EcoRI         161  GAATTC               G^AATTC
HgaI         1341  GCGTC                GACGC(5/10)
HhaI          876  GCGC                 GCG^C
HinP1I        876  GCGC                 G^CGC
HincII        208  GTTAAC               GT[CT]^[GA]AC
HindII        208  GTTAAC               GT[CT]^[GA]AC
HindIII      1102  AAGCTT               A^AGCTT
HpaI          208  GTTAAC               GTT^AAC
MfeI         1165  CAATTG               C^AATTG
MluNI        1662  TGGCCA               TGG^CCA
MscI         1662  TGGCCA               TGG^CCA
MslI          321  CATAACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1165  CAATTG               C^AATTG
PflFI        1729  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         803  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         915  CAGCTG               CAG^CTG
SacI          979  GAGCTC               GAGCT^C
SapI         1323  GAAGAGC              GCTCTTC(1/4)
ScaI         1617  AGTACT               AGT^ACT
SfcI          903  CTACAG               C^T[GA][CT]AG
SspBI         329  TGTACA               T^GTACA
Tth111I      1729  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AspHI         891  GTGCTC               G[AT]GC[AT]^C
              979  GAGCTC              
BanII         355  GAGCCC               G[GA]GC[CT]^C
              979  GAGCTC              
BcnI          462  CCCGG                CC^[GC]GG
              807  CCCGG               
BsgI          360  CTGCAC               GTGCAG(16/14)
             1609  CTGCAC              
BsiHKAI       891  GTGCTC               G[AT]GC[AT]^C
              979  GAGCTC              
BsrI          985  ACTGG                ACTGG(1/-1)
              187  CCAGT               
BstYI          95  AGATCT               [GA]^GATC[CT]
             1643  GGATCC              
Cac8I         711  GCTGGC               GC[ACGT]^[ACGT]GC
              878  GCCTGC              
EarI          386  GAAGAG               CTCTTC(1/4)
             1323  GAAGAG              
FauI          846  CCCGC                CCCGC(4/6)
              887  GCGGG               
HapII         463  CCGG                 C^CGG
              808  CCGG                
HpaII         463  CCGG                 C^CGG
              808  CCGG                
Ksp632I       386  GAAGAG               CTCTTC(1/4)
             1323  GAAGAG              
MaeII        1482  ACGT                 A^CGT
             1580  ACGT                
MflI           95  AGATCT               [GA]^GATC[CT]
             1643  GGATCC              
MspA1I        915  CAGCTG               C[AC]G^C[GT]G
              949  CCGCTG              
MspI          463  CCGG                 C^CGG
              808  CCGG                
NciI          462  CCCGG                CC^[GC]GG
              807  CCCGG               
SfaNI        1876  GATGC                GCATC(5/9)
              920  GCATC               
StuI          691  AGGCCT               AGG^CCT
             1677  AGGCCT              
TaqI          840  TCGA                 T^CGA
             1220  TCGA                
Tsp45I       1255  GTCAC                ^GT[GC]AC
             1894  GTGAC               
TthHB8I       840  TCGA                 T^CGA
             1220  TCGA                
XhoII          95  AGATCT               [GA]^GATC[CT]
             1643  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XbaI        XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com