Restriction map for pF1KE0006
 (Linear) (Six-base) MAPSORT of: KIEE0006.seq  Check: 7759  from: 1  to: 1563



KIEE0006.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0      71    1451    1563 
   Size:         71    1380     112

  Fragments arranged by size: 

                1380     112      71
 
AfeI AGC'GCT

Cuts at:      0     216    1563 
   Size:        216    1347
 
AleI CACnn'nnGTG

Cuts at:      0    1102    1563 
   Size:       1102     461
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     593     821    1563 
   Size:        593     228     742

  Fragments arranged by size: 

                 742     593     228
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     709     741     818     850    1563 
   Size:        709      32      77      32     713

  Fragments arranged by size: 

                 713     709      77      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1563 
   Size:        939     624
 
ApoI r'AATT_y

Cuts at:      0     106     157    1141    1245    1563 
   Size:        106      51     984     104     318

  Fragments arranged by size: 

                 984     318     106     104      51
 
AseI AT'TA_AT

Cuts at:      0     464    1563 
   Size:        464    1099
 
AvaI C'yCGr_G

Cuts at:      0     654    1563 
   Size:        654     909
 
BamHI G'GATC_C

Cuts at:      0     833    1563 
   Size:        833     730
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1048    1082    1563 
   Size:       1048      34     481

  Fragments arranged by size: 

                1048     481      34
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     233    1563 
   Size:        233    1330
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61    1563 
   Size:         61    1502
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697     991    1563 
   Size:        697     294     572

  Fragments arranged by size: 

                 697     572     294
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     584     666    1563 
   Size:        584      82     897

  Fragments arranged by size: 

                 897     584      82
 
BsaBI GATnn'nnATC

Cuts at:      0    1274    1563 
   Size:       1274     289
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     986    1016    1563 
   Size:        986      30     547

  Fragments arranged by size: 

                 986     547      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     225     994    1563 
   Size:         58     167     769     569

  Fragments arranged by size: 

                 769     569     167      58
 
BseYI C'CCAG_C

Cuts at:      0     194     566    1563 
   Size:        194     372     997

  Fragments arranged by size: 

                 997     372     194
 
BsrDI GCAATG_nn'

Cuts at:      0     654    1184    1563 
   Size:        654     530     379

  Fragments arranged by size: 

                 654     530     379
 
BsrGI T'GTAC_A

Cuts at:      0    1416    1563 
   Size:       1416     147
 
BstYI r'GATC_y

Cuts at:      0     774     833     998    1563 
   Size:        774      59     165     565

  Fragments arranged by size: 

                 774     565     165      59
 
BtgI C'CryG_G

Cuts at:      0     185     390    1563 
   Size:        185     205    1173

  Fragments arranged by size: 

                1173     205     185
 
BtsI GCAGTG_nn'

Cuts at:      0     705    1026    1563 
   Size:        705     321     537

  Fragments arranged by size: 

                 705     537     321
 
DraIII CAC_nnn'GTG

Cuts at:      0    1212    1563 
   Size:       1212     351
 
EaeI y'GGCC_r

Cuts at:      0     323    1563 
   Size:        323    1240
 
EarI CTCTTCn'nnn_

Cuts at:      0     811    1028    1563 
   Size:        811     217     535

  Fragments arranged by size: 

                 811     535     217
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     838    1563 
   Size:        838     725
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61    1563 
   Size:         61    1502
 
EcoO109I rG'GnC_Cy

Cuts at:      0     190    1563 
   Size:        190    1373
 
EcoRV GAT'ATC

Cuts at:      0     522    1563 
   Size:        522    1041
 
HaeII r_GCGC'y

Cuts at:      0     218    1563 
   Size:        218    1345
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     759     791     841     873    1147    1179    1563 
   Size:        759      32      50      32     274      32     384

  Fragments arranged by size: 

                 759     384     274      50      32      32      32
 
HincII GTy'rAC

Cuts at:      0     629    1563 
   Size:        629     934
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     938    1563 
   Size:        938     625
 
MscI TGG'CCA

Cuts at:      0     325    1563 
   Size:        325    1238
 
MslI CAynn'nnrTG

Cuts at:      0     283    1060    1102    1563 
   Size:        283     777      42     461

  Fragments arranged by size: 

                 777     461     283      42
 
MspA1I CmG'CkG

Cuts at:      0     198     624    1563 
   Size:        198     426     939

  Fragments arranged by size: 

                 939     426     198
 
NspI r_CATG'y

Cuts at:      0     333     478     873    1563 
   Size:        333     145     395     690

  Fragments arranged by size: 

                 690     395     333     145
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     755    1314    1563 
   Size:        755     559     249

  Fragments arranged by size: 

                 755     559     249
 
PfoI T'CCnGG_A

Cuts at:      0    1250    1307    1563 
   Size:       1250      57     256

  Fragments arranged by size: 

                1250     256      57
 
PpuMI rG'GwC_Cy

Cuts at:      0     190    1563 
   Size:        190    1373
 
PsiI TTA'TAA

Cuts at:      0     490    1563 
   Size:        490    1073
 
PstI C_TGCA'G

Cuts at:      0     935    1074    1269    1563 
   Size:        935     139     195     294

  Fragments arranged by size: 

                 935     294     195     139
 
SanDI GG'GwC_CC

Cuts at:      0     190    1563 
   Size:        190    1373
 
ScaI AGT'ACT

Cuts at:      0     971    1563 
   Size:        971     592
 
SfcI C'TryA_G

Cuts at:      0      68     414     507     597     931    1070    1265    1563 
   Size:         68     346      93      90     334     139     195     298

  Fragments arranged by size: 

                 346     334     298     195     139      93      90      68
            
 
SmlI C'TyrA_G

Cuts at:      0     712    1006    1563 
   Size:        712     294     557

  Fragments arranged by size: 

                 712     557     294
 
SphI G_CATG'C

Cuts at:      0     478    1563 
   Size:        478    1085
 
SspI AAT'ATT

Cuts at:      0    1498    1563 
   Size:       1498      65
 
TatI w'GTAC_w

Cuts at:      0     919     969    1416    1563 
   Size:        919      50     447     147

  Fragments arranged by size: 

                 919     447     147      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     890    1563 
   Size:        890     673
 

 Enzymes that do cut:

     AccI     AfeI     AleI     AlfI     AloI    AlwNI     ApoI     AseI
     AvaI    BamHI     BcgI     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaXI    BseRI    BseYI    BsrDI    BsrGI    BstYI     BtgI     BtsI
   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRV    HaeII
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NspI    PflMI
     PfoI    PpuMI     PsiI     PstI    SanDI     ScaI     SfcI     SmlI
     SphI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BsaAI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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