Restriction map for pF1KB7424
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        TaqI        
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwI         1375  GGATC                GGATC(4/5)
BanII         272  GGGCTC               G[GA]GC[CT]^C
BbsI          804  GTCTTC               GAAGAC(2/6)
BcgI          499  GCAGCTTTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         773  GGATAC               GTATCC(6/5)
BclI         1026  TGATCA               T^GATCA
BmyI          272  GGGCTC               G[AGT]GC[ACT]^C
BpuAI         804  GTCTTC               GAAGAC(2/6)
BseRI         431  CTCCTC               GAGGAG(10/8)
Bsp1286I      272  GGGCTC               G[AGT]GC[ACT]^C
BstXI         403  CCATTTAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         979  GCATGC               GC[ACGT]^[ACGT]GC
CfoI         1018  GCGC                 GCG^C
Cfr13I        178  GGGCC                G^G[ACGT]CC
DrdI            6  GACTGTACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1008  GAATTC               G^AATTC
FbaI         1026  TGATCA               T^GATCA
HaeIII        179  GGCC                 GG^CC
HapII         151  CCGG                 C^CGG
HhaI         1018  GCGC                 GCG^C
HinP1I       1018  GCGC                 G^CGC
HpaII         151  CCGG                 C^CGG
MaeII          87  ACGT                 A^CGT
MboII         805  TCTTC                GAAGA(8/7)
MslI          404  CATTTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          151  CCGG                 C^CGG
NlaIV         273  GGCTCC               GG[ACGT]^[ACGT]CC
PflMI         998  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          802  GAGTC                GAGTC(4/5)
Sau96I        178  GGGCC                G^G[ACGT]CC
ScaI           55  AGTACT               AGT^ACT
SmlI         1197  CTCAAG               C^T[CT][GA]AG
SphI          979  GCATGC               GCATG^C
SspI         1401  AATATT               AAT^ATT
SwaI          405  ATTTAAAT             ATTT^AAAT
TfiI           28  GATTC                G^A[AT]TC
Tsp45I        190  GTGAC                ^GT[GC]AC
Van91I        998  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           94  CCACATGAGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          990  AAATTC               [GA]^AATT[CT]
             1008  GAATTC              
AlwNI         923  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1292  CAGTTCCTG           
ApoI          990  AAATTC               [GA]^AATT[CT]
             1008  GAATTC              
AseI         1048  ATTAAT               AT^TAAT
             1398  ATTAAT              
AsnI         1048  ATTAAT               AT^TAAT
             1398  ATTAAT              
BsaBI          28  GATTCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1339  GATTTTAATC          
BsrI         1389  ACTGG                ACTGG(1/-1)
              778  CCAGT               
BstAPI        886  GCAGTCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              922  GCAGCAACTGC         
DdeI          342  CTTAG                C^T[ACGT]AG
             1013  CTCAG               
DraI          406  TTTAAA               TTT^AAA
             1206  TTTAAA              
DsaI          251  CCATGG               C^C[GA][CT]GG
              796  CCATGG              
HinfI          28  GATTC                G^A[ACGT]TC
              802  GAGTC               
HphI          597  GGTGA                GGTGA(8/7)
              578  TCACC               
MamI           28  GATTCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1339  GATTTTAATC          
NcoI          251  CCATGG               C^CATGG
              796  CCATGG              
NspI          632  ACATGC               [GA]CATG^[CT]
              979  GCATGC              
PshBI        1048  ATTAAT               AT^TAAT
             1398  ATTAAT              
PstI          497  CTGCAG               CTGCA^G
             1434  CTGCAG              
TspRI          78  CACTG                CA[GC]TG[ACGT][ACGT]^
             1120  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BfaI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstF5I      BstNI       DpnII       EcoRII      
EcoT14I     Fnu4HI      FokI        ItaI        MaeI        
MaeIII      MboI        MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      RsaI        Sau3AI      
ScrFI       SfaNI       SfcI        StyI        Tru9I       
TseI        Tsp509I     
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