Restriction map for pF1KB9940
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        AspEI       AspI        AvaI        
BalI        BbeI        BbrPI       BcgI        BcnI        
BfrI        BglI        BglII       BlpI        Bpu1102I    
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       FauI        FseI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       SspI        StuI        
TaqI        Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I       1157  GGTACC               G^GTACC
AcyI         1918  GGCGTC               G[GA]^CG[CT]C
AlwNI        1223  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          860  GGGCCC               GGGCC^C
Asp700I      1189  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1157  GGTACC               G^GTACC
AviII         929  TGCGCA               TGC^GCA
AvrII        1317  CCTAGG               C^CTAGG
BamHI         285  GGATCC               G^GATCC
BanI         1157  GGTACC               G^G[CT][GA]CC
BbvCI        1538  CCTCAGC              CCTCAGC(-5/-2)
BciVI         181  GGATAC               GTATCC(6/5)
BlnI         1317  CCTAGG               C^CTAGG
BpmI           61  CTGGAG               CTGGAG(16/14)
BsaAI         964  CACGTA               [CT]AC^GT[GA]
BsaBI        1176  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1918  GGCGTC               G[GA]^CG[CT]C
BsaI          803  GGTCTC               GGTCTC(1/5)
BsaWI         311  ACCGGA               [AT]^CCGG[AT]
BsmAI         804  GTCTC                GTCTC(1/5)
BsmFI         648  GGGAC                GGGAC(10/14)
BspHI        1058  TCATGA               T^CATGA
BspLU11I     1148  ACATGT               A^CATGT
BsrFI        1084  ACCGGC               [GA]^CCGG[CT]
BstXI         963  CCACGTAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         970  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I       1084  ACCGGC               [GA]^CCGG[CT]
DraI         1485  TTTAAA               TTT^AAA
DrdI          589  GACAATAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           88  CCATGG               C^C[GA][CT]GG
EcoNI        1623  CCTTGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1843  GAATTC               G^AATTC
EcoT22I      1496  ATGCAT               ATGCA^T
FspI          929  TGCGCA               TGC^GCA
HgaI         1919  GCGTC                GACGC(5/10)
Hin1I        1918  GGCGTC               G[GA]^CG[CT]C
KpnI         1157  GGTACC               GGTAC^C
MamI         1176  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI           88  CCATGG               C^CATGG
NsiI         1496  ATGCAT               ATGCA^T
PshAI         170  GACTAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1058  TCATGA               T^CATGA
SacI           36  GAGCTC               GAGCT^C
SapI           99  GCTCTTC              GCTCTTC(1/4)
ScaI          714  AGTACT               AGT^ACT
SwaI         1484  ATTTAAAT             ATTT^AAAT
Tsp45I        587  GTGAC                ^GT[GC]AC
XcmI          677  CCATGATTTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1189  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII          30  CGCG                 CG^CG
             1367  CGCG                
AflIII        936  ACGTGT               A^C[GA][CT]GT
             1148  ACATGT              
Alw44I        500  GTGCAC               G^TGCAC
             1421  GTGCAC              
ApaLI         500  GTGCAC               G^TGCAC
             1421  GTGCAC              
AvaII          66  GGACC                G^G[AT]CC
              252  GGACC               
BclI          572  TGATCA               T^GATCA
             1715  TGATCA              
BseRI          21  CTCCTC               GAGGAG(10/8)
              865  CTCCTC              
BsmI         1494  GAATGC               GAATGC(1/-1)
             1572  GAATGC              
BsrDI        1750  CATTGC               GCAATG(2/0)
             1894  GCAATG              
BsrI         1052  ACTGG                ACTGG(1/-1)
              487  CCAGT               
BssKI         297  CCTGG                ^CC[ACGT]GG
             1834  CCTGG               
BstNI         297  CCTGG                CC^[AT]GG
             1834  CCTGG               
BstUI          30  CGCG                 CG^CG
             1367  CGCG                
CfoI           31  GCGC                 GCG^C
              930  GCGC                
DraII         566  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              860  GGGCCCT             
DraIII        332  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1424  CACGATGTG           
EarI          100  CTCTTC               CTCTTC(1/4)
              109  CTCTTC              
EcoO109I      566  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              860  GGGCCCT             
EcoRII        297  CCTGG                ^CC[AT]GG
             1834  CCTGG               
FbaI          572  TGATCA               T^GATCA
             1715  TGATCA              
HapII         312  CCGG                 C^CGG
             1085  CCGG                
HhaI           31  GCGC                 GCG^C
              930  GCGC                
HinP1I         31  GCGC                 G^CGC
              930  GCGC                
HinfI         735  GATTC                G^A[ACGT]TC
             1927  GATTC               
HpaII         312  CCGG                 C^CGG
             1085  CCGG                
HphI          690  GGTGA                GGTGA(8/7)
              575  TCACC               
Ksp632I       100  CTCTTC               CTCTTC(1/4)
              109  CTCTTC              
MaeII         936  ACGT                 A^CGT
              965  ACGT                
MspA1I         58  CCGCTG               C[AC]G^C[GT]G
              262  CAGCGG              
MspI          312  CCGG                 C^CGG
             1085  CCGG                
MvaI          297  CCTGG                CC^[AT]GG
             1834  CCTGG               
MvnI           30  CGCG                 CG^CG
             1367  CGCG                
NspI         1148  ACATGT               [GA]CATG^[CT]
             1201  GCATGT              
PflMI         238  CCAAACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1161  CCATGTGGTGG         
ScrFI         297  CCTGG                CC^[ACGT]GG
             1834  CCTGG               
SfaNI        1549  GATGC                GCATC(5/9)
             1288  GCATC               
SpeI          171  ACTAGT               A^CTAGT
              666  ACTAGT              
TfiI          735  GATTC                G^A[AT]TC
             1927  GATTC               
Van91I        238  CCAAACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1161  CCATGTGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BanII       BbsI        BbvI        
BfaI        BmyI        BpuAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        Bsp1286I    BstAPI      
BstF5I      BstYI       Cfr13I      DdeI        DpnII       
EcoT14I     Fnu4HI      FokI        HaeIII      ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MwoI        NdeII       
NlaIII      NlaIV       PstI        RsaI        Sau3AI      
Sau96I      SfcI        StyI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       
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