Restriction map for pF1KB9940
 (Linear) (Six-base) MAPSORT of: KIBB9940.seq  Check: 7339  from: 1  to: 1971



KIBB9940.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

Acc65I G'GTAC_C

Cuts at:      0    1157    1971 
   Size:       1157     814
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     866    1971 
   Size:        820      46    1105

  Fragments arranged by size: 

                1105     820      46
 
AflIII A'CryG_T

Cuts at:      0     936    1148    1971 
   Size:        936     212     823

  Fragments arranged by size: 

                 936     823     212
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1228    1971 
   Size:       1228     743
 
ApaI G_GGCC'C

Cuts at:      0     864    1971 
   Size:        864    1107
 
ApaLI G'TGCA_C

Cuts at:      0     500    1421    1971 
   Size:        500     921     550

  Fragments arranged by size: 

                 921     550     500
 
ApoI r'AATT_y

Cuts at:      0    1127    1189    1263    1361    1843    1971 
   Size:       1127      62      74      98     482     128

  Fragments arranged by size: 

                1127     482     128      98      74      62
 
AvrII C'CTAG_G

Cuts at:      0    1317    1971 
   Size:       1317     654
 
BamHI G'GATC_C

Cuts at:      0     285    1971 
   Size:        285    1686
 
BanI G'GyrC_C

Cuts at:      0    1157    1971 
   Size:       1157     814
 
BanII G_rGCy'C

Cuts at:      0      40     864    1834    1971 
   Size:         40     824     970     137

  Fragments arranged by size: 

                 970     824     137      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     854     972     996    1971 
   Size:        854     118      24     975

  Fragments arranged by size: 

                 975     854     118      24
 
BbvCI CC'TCA_GC

Cuts at:      0    1539    1971 
   Size:       1539     432
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     175    1971 
   Size:        175    1796
 
BclI T'GATC_A

Cuts at:      0     572    1715    1971 
   Size:        572    1143     256

  Fragments arranged by size: 

                1143     572     256
 
BfrBI ATG'CAT

Cuts at:      0    1498    1971 
   Size:       1498     473
 
Bme1580I G_kGCm'C

Cuts at:      0     504     864    1425    1971 
   Size:        504     360     561     546

  Fragments arranged by size: 

                 561     546     504     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1971 
   Size:         82    1889
 
Bpu10I CC'TnA_GC

Cuts at:      0     827    1539    1971 
   Size:        827     712     432

  Fragments arranged by size: 

                 827     712     432
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     809    1971 
   Size:        809    1162
 
BsaAI yAC'GTr

Cuts at:      0     966    1971 
   Size:        966    1005
 
BsaBI GATnn'nnATC

Cuts at:      0    1180    1971 
   Size:       1180     791
 
BsaHI Gr'CG_yC

Cuts at:      0    1919    1971 
   Size:       1919      52
 
BsaWI w'CCGG_w

Cuts at:      0     311    1971 
   Size:        311    1660
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     438     468     807     837    1217    1247    1971 
   Size:        438      30     339      30     380      30     724

  Fragments arranged by size: 

                 724     438     380     339      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12     856    1971 
   Size:         12     844    1115

  Fragments arranged by size: 

                1115     844      12
 
BseYI C'CCAG_C

Cuts at:      0     260    1660    1971 
   Size:        260    1400     311

  Fragments arranged by size: 

                1400     311     260
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     179    1518    1971 
   Size:         60     119    1339     453

  Fragments arranged by size: 

                1339     453     119      60
 
BsiHKAI G_wGCw'C

Cuts at:      0      40     504     531    1425    1971 
   Size:         40     464      27     894     546

  Fragments arranged by size: 

                 894     546     464      40      27
 
BsmI GAATG_Cn'

Cuts at:      0    1500    1578    1971 
   Size:       1500      78     393

  Fragments arranged by size: 

                1500     393      78
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     504     531     864    1425    1834    1971 
   Size:         40     464      27     333     561     409     137

  Fragments arranged by size: 

                 561     464     409     333     137      40      27
 
BspHI T'CATG_A

Cuts at:      0    1058    1971 
   Size:       1058     913
 
BsrDI GCAATG_nn'

Cuts at:      0    1749    1901    1971 
   Size:       1749     152      70

  Fragments arranged by size: 

                1749     152      70
 
BsrFI r'CCGG_y

Cuts at:      0    1084    1971 
   Size:       1084     887
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     157    1451    1549    1966    1971 
   Size:        157    1294      98     417       5

  Fragments arranged by size: 

                1294     417     157      98       5
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     970    1971 
   Size:        970    1001
 
BstYI r'GATC_y

Cuts at:      0     285     725    1336    1971 
   Size:        285     440     611     635

  Fragments arranged by size: 

                 635     611     440     285
 
BtgI C'CryG_G

Cuts at:      0      88    1971 
   Size:         88    1883
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     505     936    1971 
   Size:        505     431    1035

  Fragments arranged by size: 

                1035     505     431
 
BtsI GCAGTG_nn'

Cuts at:      0     994    1971 
   Size:        994     977
 
DraI TTT'AAA

Cuts at:      0    1487    1971 
   Size:       1487     484
 
DraIII CAC_nnn'GTG

Cuts at:      0     337    1429    1971 
   Size:        337    1092     542

  Fragments arranged by size: 

                1092     542     337
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     595    1971 
   Size:        595    1376
 
EarI CTCTTCn'nnn_

Cuts at:      0     106     115    1971 
   Size:        106       9    1856

  Fragments arranged by size: 

                1856     106       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       6      15    1971 
   Size:          6       9    1956

  Fragments arranged by size: 

                1956       9       6
 
EcoICRI GAG'CTC

Cuts at:      0      38    1971 
   Size:         38    1933
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     820     866    1971 
   Size:         82     738      46    1105

  Fragments arranged by size: 

                1105     738      82      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1627    1971 
   Size:       1627     344
 
EcoO109I rG'GnC_Cy

Cuts at:      0     567     861    1971 
   Size:        567     294    1110

  Fragments arranged by size: 

                1110     567     294
 
EcoRI G'AATT_C

Cuts at:      0    1843    1971 
   Size:       1843     128
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     871    1569    1971 
   Size:        871     698     402

  Fragments arranged by size: 

                 871     698     402
 
FspI TGC'GCA

Cuts at:      0     931    1971 
   Size:        931    1040
 
KpnI G_GTAC'C

Cuts at:      0    1161    1971 
   Size:       1161     810
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1032    1971 
   Size:        206     826     939

  Fragments arranged by size: 

                 939     826     206
 
MslI CAynn'nnrTG

Cuts at:      0     121     524    1564    1971 
   Size:        121     403    1040     407

  Fragments arranged by size: 

                1040     407     403     121
 
MspA1I CmG'CkG

Cuts at:      0      60     264    1971 
   Size:         60     204    1707

  Fragments arranged by size: 

                1707     204      60
 
NcoI C'CATG_G

Cuts at:      0      88    1971 
   Size:         88    1883
 
NsiI A_TGCA'T

Cuts at:      0    1500    1971 
   Size:       1500     471
 
NspI r_CATG'y

Cuts at:      0    1152    1205    1971 
   Size:       1152      53     766

  Fragments arranged by size: 

                1152     766      53
 
PciI A'CATG_T

Cuts at:      0    1148    1971 
   Size:       1148     823
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     244    1167    1971 
   Size:        244     923     804

  Fragments arranged by size: 

                 923     804     244
 
PshAI GACnn'nnGTC

Cuts at:      0     174    1971 
   Size:        174    1797
 
PspOMI G'GGCC_C

Cuts at:      0     860    1971 
   Size:        860    1111
 
PstI C_TGCA'G

Cuts at:      0      13    1145    1648    1971 
   Size:         13    1132     503     323

  Fragments arranged by size: 

                1132     503     323      13
 
SacI G_AGCT'C

Cuts at:      0      40    1971 
   Size:         40    1931
 
SapI GCTCTTCn'nnn_

Cuts at:      0     106    1971 
   Size:        106    1865
 
ScaI AGT'ACT

Cuts at:      0     716    1971 
   Size:        716    1255
 
SfcI C'TryA_G

Cuts at:      0       9     326     887    1141    1644    1971 
   Size:          9     317     561     254     503     327

  Fragments arranged by size: 

                 561     503     327     317     254       9
 
SpeI A'CTAG_T

Cuts at:      0     171     666    1971 
   Size:        171     495    1305

  Fragments arranged by size: 

                1305     495     171
 
StyI C'CwwG_G

Cuts at:      0      88     352    1317    1623    1971 
   Size:         88     264     965     306     348

  Fragments arranged by size: 

                 965     348     306     264      88
 
SwaI ATTT'AAAT

Cuts at:      0    1487    1971 
   Size:       1487     484
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     274    1035    1692    1971 
   Size:        274     761     657     279

  Fragments arranged by size: 

                 761     657     279     274
 
TatI w'GTAC_w

Cuts at:      0     714     771    1049    1145    1971 
   Size:        714      57     278      96     826

  Fragments arranged by size: 

                 826     714     278      96      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     438     470    1971 
   Size:        438      32    1501

  Fragments arranged by size: 

                1501     438      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     684    1971 
   Size:        684    1287
 
XmnI GAAnn'nnTTC

Cuts at:      0    1193    1971 
   Size:       1193     778
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI    AvrII
    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI
 Bme1580I     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI     KpnI     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI     PciI    PflMI    PshAI   PspOMI     PstI
     SacI     SapI     ScaI     SfcI     SpeI     StyI     SwaI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI     AvaI     BaeI
     BbeI     BcgI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     EaeI     EagI    EcoRV     FseI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     SspI     StuI  Tth111I     XbaI     XhoI     XmaI     ZraI
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