Restriction map for pF1KB9921
 (Circular) (Six-base) MAPSORT of: pF1KB9921.seq  Check: 4994  from: 1  to: 4124



pF1KB9921  4124 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:    782    1162     782 
   Size:        380    3744
 
Acc65I G'GTAC_C

Cuts at:   1140    1140 
   Size:       4124
 
AclI AA'CG_TT

Cuts at:   3964    3964 
   Size:       4124
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    950    1949    2381    3128    3396     950 
   Size:        999     432     747     268    1678

  Fragments arranged by size: 

                1678     999     747     432     268
 
AflIII A'CryG_T

Cuts at:   1476    2580    3557    1476 
   Size:       1104     977    2043

  Fragments arranged by size: 

                2043    1104     977
 
AgeI A'CCGG_T

Cuts at:   2512    2512 
   Size:       4124
 
AleI CACnn'nnGTG

Cuts at:    247     247 
   Size:       4124
 
AlwNI CAG_nnn'CTG

Cuts at:   1429    2996    1429 
   Size:       1567    2557
 
ApaLI G'TGCA_C

Cuts at:   2894    2894 
   Size:       4124
 
ApoI r'AATT_y

Cuts at:    290    1128    3477    3733     290 
   Size:        838    2349     256     681

  Fragments arranged by size: 

                2349     838     681     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4124
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4124
 
AvaI C'yCGr_G

Cuts at:    117    1144     117 
   Size:       1027    3097
 
BamHI G'GATC_C

Cuts at:   1149    1149 
   Size:       4124
 
BanI G'GyrC_C

Cuts at:   1140    1554    1802    1837    1140 
   Size:        414     248      35    3427

  Fragments arranged by size: 

                3427     414     248      35
 
BanII G_rGCy'C

Cuts at:    361    1138    2168     361 
   Size:        777    1030    2317

  Fragments arranged by size: 

                2317    1030     777
 
BbeI G_GCGC'C

Cuts at:   1558    1806    1558 
   Size:        248    3876
 
BciVI GTATCCnnnnn_n'

Cuts at:   2022    2783    4124    2022 
   Size:        761    1341    2022

  Fragments arranged by size: 

                2022    1341     761
 
BclI T'GATC_A

Cuts at:   1644    1644 
   Size:       4124
 
BglII A'GATC_T

Cuts at:   1639    3676    1639 
   Size:       2037    2087
 
BlpI GC'TnA_GC

Cuts at:   1236    1236 
   Size:       4124
 
Bme1580I G_kGCm'C

Cuts at:   1749    1842    2898    1749 
   Size:         93    1056    2975

  Fragments arranged by size: 

                2975    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1336    1417    1752    3896    1336 
   Size:         81     335    2144    1564

  Fragments arranged by size: 

                2144    1564     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2491    2491 
   Size:       4124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     587 
   Size:       4124
 
Bpu10I CC'TnA_GC

Cuts at:    669     669 
   Size:       4124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1300    2179    2671    2969    1300 
   Size:        879     492     298    2455

  Fragments arranged by size: 

                2455     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3828    3828 
   Size:       4124
 
BsaAI yAC'GTr

Cuts at:   1477    1477 
   Size:       4124
 
BsaBI GATnn'nnATC

Cuts at:      6    1638    3675       6 
   Size:       1632    2037     455

  Fragments arranged by size: 

                2037    1632     455
 
BsaHI Gr'CG_yC

Cuts at:    779    1555    1803    2505    4001     779 
   Size:        776     248     702    1496     902

  Fragments arranged by size: 

                1496     902     776     702     248
 
BsaWI w'CCGG_w

Cuts at:    518    1313    1834    2512    2786    2933    3344     518 
   Size:        795     521     678     274     147     411    1298

  Fragments arranged by size: 

                1298     795     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1549    2307    2884    3380    1549 
   Size:        758     577     496    2293

  Fragments arranged by size: 

                2293     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1666    1712    2920     104 
   Size:       1562      46    1208    1308

  Fragments arranged by size: 

                1562    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    670    1138    1916    2106    2898     670 
   Size:        468     778     190     792    1896

  Fragments arranged by size: 

                1896     792     778     468     190
 
Bsp1286I G_dGCh'C

Cuts at:    361     670    1138    1749    1842    1916    2106    2168    2898 
   Size:        309     468     611      93      74     190      62     730

Cuts at:   2898     361 
   Size:       1587

  Fragments arranged by size: 

                1587     730     611     468     309     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3344    3344 
   Size:       4124
 
BspHI T'CATG_A

Cuts at:    917    3300     917 
   Size:       2383    1741
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1174    1388    1690    2071    1174 
   Size:        214     302     381    3227

  Fragments arranged by size: 

                3227     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2416    2470    3954    4119      50 
   Size:       2366      54    1484     165      55

  Fragments arranged by size: 

                2366    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    959    2036     959 
   Size:       1077    3047
 
BsrFI r'CCGG_y

Cuts at:   2122    2303    2512    2122 
   Size:        181     209    3734

  Fragments arranged by size: 

                3734     209     181
 
BsrGI T'GTAC_A

Cuts at:    579     579 
   Size:       4124
 
BssSI C'ACGA_G

Cuts at:   2395    2753    2395 
   Size:        358    3766
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1501    1501 
   Size:       4124
 
BstBI TT'CG_AA

Cuts at:   2485    2485 
   Size:       4124
 
BstEII G'GTnAC_C

Cuts at:    462     462 
   Size:       4124
 
BstYI r'GATC_y

Cuts at:      1    1149    1616    1639    1974    2220    3232    3318    3330 
   Size:       1148     467      23     335     246    1012      86      12

Cuts at:   3330    3676       1 
   Size:        346     449

  Fragments arranged by size: 

                1148    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4124
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2074    2255    2418    2074 
   Size:        181     163    3780

  Fragments arranged by size: 

                3780     181     163
 
BtsI GCAGTG_nn'

Cuts at:    167     458    1367     167 
   Size:        291     909    2924

  Fragments arranged by size: 

                2924     909     291
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    593     628     593 
   Size:         35    4089
 
DraI TTT'AAA

Cuts at:    704    1123     704 
   Size:        419    3705
 
DrdI GACnn_nn'nnGTC

Cuts at:   1830    2688    1830 
   Size:        858    3266
 
EaeI y'GGCC_r

Cuts at:    941    1709    1883    2274    2301    3646     941 
   Size:        768     174     391      27    1345    1419

  Fragments arranged by size: 

                1419    1345     768     391     174      27
 
EagI C'GGCC_G

Cuts at:   1709    1709 
   Size:       4124
 
EarI CTCTTCn'nnn_

Cuts at:   1022    2147    2357    1022 
   Size:       1125     210    2789

  Fragments arranged by size: 

                2789    1125     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2332    2642    2788    3934    2332 
   Size:        310     146    1146    2522

  Fragments arranged by size: 

                2522    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1136    1136 
   Size:       4124
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     950    1949    2381    3128    3396     587 
   Size:        363     999     432     747     268    1315

  Fragments arranged by size: 

                1315     999     747     432     363     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1263    1263 
   Size:       4124
 
EcoRI G'AATT_C

Cuts at:   1128    1128 
   Size:       4124
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2143    2143 
   Size:       4124
 
FspI TGC'GCA

Cuts at:   1905    1905 
   Size:       4124
 
HaeII r_GCGC'y

Cuts at:    647    1558    1806    2828    3807     647 
   Size:        911     248    1022     979     964

  Fragments arranged by size: 

                1022     979     964     911     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    113     145     332     364    1054    1086    1966    1996    1998 
   Size:         32     187      32     690      32     880      30       2

Cuts at:   1998    2028     113 
   Size:         30    2209

  Fragments arranged by size: 

                2209     880     690     187      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1163    1163 
   Size:       4124
 
KasI G'GCGC_C

Cuts at:   1554    1802    1554 
   Size:        248    3876
 
KpnI G_GTAC'C

Cuts at:   1144    1144 
   Size:       4124
 
MluI A'CGCG_T

Cuts at:   3557    3557 
   Size:       4124
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1101    2795    2979    1101 
   Size:       1694     184    2246

  Fragments arranged by size: 

                2246    1694     184
 
MscI TGG'CCA

Cuts at:   1885    1885 
   Size:       4124
 
MslI CAynn'nnrTG

Cuts at:    247     742    2113    2240     247 
   Size:        495    1371     127    2131

  Fragments arranged by size: 

                2131    1371     495     127
 
MspA1I CmG'CkG

Cuts at:    940    1235    1549    1909    2922    3167     940 
   Size:        295     314     360    1013     245    1897

  Fragments arranged by size: 

                1897    1013     360     314     295     245
 
NaeI GCC'GGC

Cuts at:   2305    2305 
   Size:       4124
 
NarI GG'CG_CC

Cuts at:   1555    1803    1555 
   Size:        248    3876
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4124
 
NgoMIV G'CCGG_C

Cuts at:   2303    2303 
   Size:       4124
 
NspI r_CATG'y

Cuts at:   1177    1677    2584    1177 
   Size:        500     907    2717

  Fragments arranged by size: 

                2717     907     500
 
PasI CC'CwG_GG

Cuts at:   1008    1008 
   Size:       4124
 
PciI A'CATG_T

Cuts at:   2580    2580 
   Size:       4124
 
PmeI GTTT'AAAC

Cuts at:   1123    1123 
   Size:       4124
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3263    3295    3263 
   Size:         32    4092
 
PsiI TTA'TAA

Cuts at:    268     268 
   Size:       4124
 
PstI C_TGCA'G

Cuts at:    183     936    1171    1856     183 
   Size:        753     235     685    2451

  Fragments arranged by size: 

                2451     753     685     235
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4124
 
PvuII CAG'CTG

Cuts at:   1549    1909    1549 
   Size:        360    3764
 
RsrII CG'GwC_CG

Cuts at:   2319    2319 
   Size:       4124
 
SacI G_AGCT'C

Cuts at:   1138    1138 
   Size:       4124
 
SalI G'TCGA_C

Cuts at:   1161    1161 
   Size:       4124
 
SapI GCTCTTCn'nnn_

Cuts at:   2147    2357    2147 
   Size:        210    3914
 
SbfI CC_TGCA'GG

Cuts at:   1171    1171 
   Size:       4124
 
ScaI AGT'ACT

Cuts at:   3641    3641 
   Size:       4124
 
SexAI A'CCwGG_T

Cuts at:    458     458 
   Size:       4124
 
SfcI C'TryA_G

Cuts at:     33     179     647     932    1167    1852    2845    3036      33 
   Size:        146     468     285     235     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     468     285     235     191     146
            
 
SfoI GGC'GCC

Cuts at:   1556    1804    1556 
   Size:        248    3876
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4124
 
SmaI CCC'GGG

Cuts at:   1146    1146 
   Size:       4124
 
SmlI C'TyrA_G

Cuts at:   1279    2194    2686    2948    1279 
   Size:        915     492     262    2455

  Fragments arranged by size: 

                2455     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4124
 
SphI G_CATG'C

Cuts at:   1177    1677    1177 
   Size:        500    3624
 
StyI C'CwwG_G

Cuts at:    107     362    1258    1612     107 
   Size:        255     896     354    2619

  Fragments arranged by size: 

                2619     896     354     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    717    1707    1840    2299    2508     717 
   Size:        990     133     459     209    2333

  Fragments arranged by size: 

                2333     990     459     209     133
 
TatI w'GTAC_w

Cuts at:    487     579     902    3639     487 
   Size:         92     323    2737     972

  Fragments arranged by size: 

                2737     972     323      92
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3641    3673    3641 
   Size:         32    4092
 
Tth111I GACn'n_nGTC

Cuts at:    127    1921     127 
   Size:       1794    2330
 
XbaI T'CTAG_A

Cuts at:   1155    1155 
   Size:       4124
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1619    1619 
   Size:       4124
 
XmaI C'CCGG_G

Cuts at:   1144    1144 
   Size:       4124
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI     PmeI     PpiI     PsiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
   BssHII    BstXI  BstZ17I   Bsu36I     ClaI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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