Restriction map for pF1KB9919
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AspHI         394  GAGCAC               G[AT]GC[AT]^C
BclI          500  TGATCA               T^GATCA
BsaWI         660  TCCGGT               [AT]^CCGG[AT]
BsgI          139  CTGCAC               GTGCAG(16/14)
BsiHKAI       394  GAGCAC               G[AT]GC[AT]^C
BspMI         516  ACCTGC               ACCTGC(4/8)
BstAPI        578  GCACTGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII        579  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          822  CCATGG               C^C[GA][CT]GG
FbaI          500  TGATCA               T^GATCA
HaeII         198  GGCGCT               [GA]GCGC^[CT]
HgaI          158  GACGC                GACGC(5/10)
MaeIII        764  GTGAC                ^GT[ACGT]AC
MboII         837  GAAGA                GAAGA(8/7)
MseI          887  TTAA                 T^TAA
NcoI          822  CCATGG               C^CATGG
NspI          716  GCATGT               [GA]CATG^[CT]
PflMI         568  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         569  CAGCTG               CAG^CTG
SexAI         281  ACCAGGT              A^CC[AT]GGT
SmlI          736  CTTGAG               C^T[CT][GA]AG
TfiI          751  GATTC                G^A[AT]TC
Tru9I         887  TTAA                 T^TAA
Tsp45I        764  GTGAC                ^GT[GC]AC
Van91I        568  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          853  GTCTAC               GT^[AC][GT]AC
              865  GTCTAC              
AccII         176  CGCG                 CG^CG
              535  CGCG                
AcsI          267  GAATTC               [GA]^AATT[CT]
              294  GAATTC              
AlwNI         209  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              569  CAGCTGCTG           
ApoI          267  GAATTC               [GA]^AATT[CT]
              294  GAATTC              
AvaII           7  GGACC                G^G[AT]CC
               40  GGACC               
BcnI           37  CCGGG                CC^[GC]GG
               43  CCGGG               
BmyI          204  GGGCAC               G[AGT]GC[ACT]^C
              394  GAGCAC              
BseRI         510  CTCCTC               GAGGAG(10/8)
              262  GAGGAG              
BsmAI         384  GAGAC                GTCTC(1/5)
              811  GTCTC               
Bsp1286I      204  GGGCAC               G[AGT]GC[ACT]^C
              394  GAGCAC              
BsrBI         100  GAGCGG               CCGCTC(-3/-3)
              170  GAGCGG              
BsrI          387  ACTGG                ACTGG(1/-1)
              881  ACTGG               
BstUI         176  CGCG                 CG^CG
              535  CGCG                
DpnII         447  GATC                 ^GATC
              501  GATC                
EaeI           34  TGGCCG               [CT]^GGCC[GA]
              440  CGGCCA              
EcoRI         267  GAATTC               G^AATTC
              294  GAATTC              
HphI          381  GGTGA                GGTGA(8/7)
              514  TCACC               
MboI          447  GATC                 ^GATC
              501  GATC                
MslI          375  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              823  CATGGCTGTG          
MspA1I        115  CAGCGG               C[AC]G^C[GT]G
              569  CAGCTG              
MvnI          176  CGCG                 CG^CG
              535  CGCG                
NciI           37  CCGGG                CC^[GC]GG
               43  CCGGG               
NdeII         447  GATC                 ^GATC
              501  GATC                
PleI          766  GACTC                GAGTC(4/5)
              851  GAGTC               
Sau3AI        447  GATC                 ^GATC
              501  GATC                
StuI           85  AGGCCT               AGG^CCT
              844  AGGCCT              
Tsp509I       268  AATT                 ^AATT
              295  AATT                
XcmI          306  CCACTTCTGCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              759  CCAGCGTGACTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        BbvI        BpmI        BsaJI       
BsiYI       BslI        BsmFI       BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MnlI        MspI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       StyI        TseI        TspRI       
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