Restriction map for pF1KB9919
 (Linear) (Six-base) MAPSORT of: KIBB9919.seq  Check: 3309  from: 1  to: 912



KIBB9919.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     525     912 
   Size:        525     387
 
AccI GT'mk_AC

Cuts at:      0     854     866     912 
   Size:        854      12      46

  Fragments arranged by size: 

                 854      46      12
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     912 
   Size:        664     248
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     207     375     912 
   Size:        207     168     537

  Fragments arranged by size: 

                 537     207     168
 
AlwNI CAG_nnn'CTG

Cuts at:      0     214     574     912 
   Size:        214     360     338

  Fragments arranged by size: 

                 360     338     214
 
ApoI r'AATT_y

Cuts at:      0     267     294     912 
   Size:        267      27     618

  Fragments arranged by size: 

                 618     267      27
 
BclI T'GATC_A

Cuts at:      0     500     912 
   Size:        500     412
 
Bme1580I G_kGCm'C

Cuts at:      0     208     912 
   Size:        208     704
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     397     912 
   Size:        397     515
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     433     551     869     912 
   Size:        154     279     118     318      43

  Fragments arranged by size: 

                 318     279     154     118      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     912 
   Size:        757     155
 
BsaWI w'CCGG_w

Cuts at:      0     660     912 
   Size:        660     252
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     549     579     912 
   Size:        549      30     333

  Fragments arranged by size: 

                 549     333      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     501     912 
   Size:        277     224     411

  Fragments arranged by size: 

                 411     277     224
 
BseYI C'CCAG_C

Cuts at:      0     201     599     912 
   Size:        201     398     313

  Fragments arranged by size: 

                 398     313     201
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     912 
   Size:        124     788
 
BsiHKAI G_wGCw'C

Cuts at:      0     398     912 
   Size:        398     514
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     398     912 
   Size:        208     190     514

  Fragments arranged by size: 

                 514     208     190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     525     912 
   Size:        525     387
 
BsrBI CCG'CTC

Cuts at:      0     102     172     912 
   Size:        102      70     740

  Fragments arranged by size: 

                 740     102      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     584     912 
   Size:        584     328
 
BtgI C'CryG_G

Cuts at:      0     822     912 
   Size:        822      90
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     882     912 
   Size:        882      30
 
BtsI GCAGTG_nn'

Cuts at:      0     357     912 
   Size:        357     555
 
DraIII CAC_nnn'GTG

Cuts at:      0     584     912 
   Size:        584     328
 
EaeI y'GGCC_r

Cuts at:      0      34     440     912 
   Size:         34     406     472

  Fragments arranged by size: 

                 472     406      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     442     912 
   Size:        442     470
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     433     551     664     869     912 
   Size:        154     279     118     113     205      43

  Fragments arranged by size: 

                 279     205     154     118     113      43
 
EcoRI G'AATT_C

Cuts at:      0     267     294     912 
   Size:        267      27     618

  Fragments arranged by size: 

                 618     267      27
 
HaeII r_GCGC'y

Cuts at:      0     202     912 
   Size:        202     710
 
MslI CAynn'nnrTG

Cuts at:      0     379     827     912 
   Size:        379     448      85

  Fragments arranged by size: 

                 448     379      85
 
MspA1I CmG'CkG

Cuts at:      0     117     571     912 
   Size:        117     454     341

  Fragments arranged by size: 

                 454     341     117
 
NcoI C'CATG_G

Cuts at:      0     822     912 
   Size:        822      90
 
NspI r_CATG'y

Cuts at:      0     720     912 
   Size:        720     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     574     912 
   Size:        574     338
 
PfoI T'CCnGG_A

Cuts at:      0     410     912 
   Size:        410     502
 
PvuII CAG'CTG

Cuts at:      0     571     912 
   Size:        571     341
 
SexAI A'CCwGG_T

Cuts at:      0     281     912 
   Size:        281     631
 
SmlI C'TyrA_G

Cuts at:      0     736     912 
   Size:        736     176
 
StuI AGG'CCT

Cuts at:      0      87     846     912 
   Size:         87     759      66

  Fragments arranged by size: 

                 759      87      66
 
StyI C'CwwG_G

Cuts at:      0     801     822     860     912 
   Size:        801      21      38      52

  Fragments arranged by size: 

                 801      52      38      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     142     330     912 
   Size:        142     188     582

  Fragments arranged by size: 

                 582     188     142
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     549     581     912 
   Size:        549      32     331

  Fragments arranged by size: 

                 549     331      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     313     766     912 
   Size:        313     453     146

  Fragments arranged by size: 

                 453     313     146
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI     ApoI     BclI Bme1580I
     BmrI     BpmI    BpuEI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI   BstAPI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EciI  Eco57MI    EcoRI    HaeII     MslI   MspA1I
     NcoI     NspI    PflMI     PfoI    PvuII    SexAI     SmlI     StuI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI     EarI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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