Restriction map for pF1KB9913
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        AlwNI       Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
CelII       CfoI        ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MspA1I      MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tth111I     TthHB8I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          802  GTCTAC               GT^[AC][GT]AC
AccII          33  CGCG                 CG^CG
AccIII         12  TCCGGA               T^CCGGA
Alw44I        774  GTGCAC               G^TGCAC
AocI          263  CCTCAGG              CC^T[ACGT]AGG
ApaLI         774  GTGCAC               G^TGCAC
Asp700I       817  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         774  GTGCAC               G[AT]GC[AT]^C
BbsI          340  GAAGAC               GAAGAC(2/6)
BcnI          714  CCGGG                CC^[GC]GG
BmyI          774  GTGCAC               G[AGT]GC[ACT]^C
BpuAI         340  GAAGAC               GAAGAC(2/6)
BseAI          12  TCCGGA               T^CCGGA
BsiHKAI       774  GTGCAC               G[AT]GC[AT]^C
BsmAI         739  GAGAC                GTCTC(1/5)
BsmFI         483  GGGAC                GGGAC(10/14)
Bsp1286I      774  GTGCAC               G[AGT]GC[ACT]^C
BspEI          12  TCCGGA               T^CCGGA
BsrDI         610  GCAATG               GCAATG(2/0)
BstNI         446  CCTGG                CC^[AT]GG
BstUI          33  CGCG                 CG^CG
BstXI         788  CCACTGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         256  GGATCT               [GA]^GATC[CT]
Bsu36I        263  CCTCAGG              CC^T[ACGT]AGG
DsaI          474  CCATGG               C^C[GA][CT]GG
EarI           40  GAAGAG               CTCTTC(1/4)
Eco81I        263  CCTCAGG              CC^T[ACGT]AGG
EcoRII        446  CCTGG                ^CC[AT]GG
EcoT14I       474  CCATGG               C^C[AT][AT]GG
FauI          687  GCGGG                CCCGC(4/6)
HaeIII        444  GGCC                 GG^CC
HincII        500  GTCAAC               GT[CT]^[GA]AC
HindII        500  GTCAAC               GT[CT]^[GA]AC
Ksp632I        40  GAAGAG               CTCTTC(1/4)
MaeII         498  ACGT                 A^CGT
MflI          256  GGATCT               [GA]^GATC[CT]
MroI           12  TCCGGA               T^CCGGA
MslI          789  CACTGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          446  CCTGG                CC^[AT]GG
MvnI           33  CGCG                 CG^CG
NaeI           60  GCCGGC               GCC^GGC
NciI          714  CCGGG                CC^[GC]GG
NcoI          474  CCATGG               C^CATGG
NgoMIV         60  GCCGGC               G^CCGGC
PflMI         474  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI           40  GAAGAGC              GCTCTTC(1/4)
StuI          443  AGGCCT               AGG^CCT
StyI          474  CCATGG               C^C[AT][AT]GG
Tsp45I        358  GTGAC                ^GT[GC]AC
Van91I        474  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          215  CCATGAAAACAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         256  GGATCT               [GA]^GATC[CT]
XmnI          817  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AciI           34  GCGG                 CCGC(-3/-1)
              687  GCGG                
AcsI          178  GAATTT               [GA]^AATT[CT]
              207  AAATTC              
AfaI          300  GTAC                 GT^AC
              352  GTAC                
AlwI          418  GATCC                GGATC(4/5)
              256  GGATC               
ApoI          178  GAATTT               [GA]^AATT[CT]
              207  AAATTC              
AvaII         484  GGACC                G^G[AT]CC
              690  GGTCC               
BbvI           71  GCAGC                GCAGC(8/12)
              724  GCTGC               
BfaI           47  CTAG                 C^TAG
              167  CTAG                
BsaWI          12  TCCGGA               [AT]^CCGG[AT]
              148  ACCGGA              
BseRI          55  GAGGAG               GAGGAG(10/8)
              643  GAGGAG              
BsrFI           5  ACCGGC               [GA]^CCGG[CT]
               60  GCCGGC              
BsrI          253  ACTGG                ACTGG(1/-1)
              766  CCAGT               
BssKI         446  CCTGG                ^CC[ACGT]GG
              714  CCGGG               
BstEII        357  GGTGACC              G^GT[ACGT]ACC
              469  GGTTACC             
BstPI         357  GGTGACC              G^GT[ACGT]ACC
              469  GGTTACC             
Cfr10I          5  ACCGGC               [GA]^CCGG[CT]
               60  GCCGGC              
Cfr13I        484  GGACC                G^G[ACGT]CC
              690  GGTCC               
EcoO65I       357  GGTGACC              G^GT[ACGT]ACC
              469  GGTTACC             
Fnu4HI         71  GCAGC                GC^[ACGT]GC
              724  GCTGC               
HphI          357  GGTGA                GGTGA(8/7)
              762  TCACC               
ItaI           71  GCAGC                GC^[ACGT]GC
              724  GCTGC               
MaeI           47  CTAG                 C^TAG
              167  CTAG                
MaeIII        358  GTGAC                ^GT[ACGT]AC
              470  GTTAC               
MseI          205  TTAA                 T^TAA
              635  TTAA                
MwoI          566  GCCTTGATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              727  GCTGAGAAGGC         
RsaI          300  GTAC                 GT^AC
              352  GTAC                
Sau96I        484  GGACC                G^G[ACGT]CC
              690  GGTCC               
ScrFI         446  CCTGG                CC^[ACGT]GG
              714  CCGGG               
Tru9I         205  TTAA                 T^TAA
              635  TTAA                
TseI           71  GCAGC                G^C[AT]GC
              724  GCTGC               
TspRI         777  CACTG                CA[GC]TG[ACGT][ACGT]^
              789  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BstF5I      
Cac8I       DdeI        DpnII       FokI        HapII       
HinfI       HpaII       MboI        MboII       MnlI        
MspI        NdeII       NlaIII      NlaIV       Sau3AI      
SfaNI       TfiI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com