Restriction map for pF1KB9906
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BamHI       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      ClaI        CpoI        DraII       
Eam1105I    Eco47III    EcoO109I    EcoO65I     EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MroI        MslI        MunI        NaeI        NgoMIV      
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PstI        
PvuI        RsrII       SacI        SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        TaqI        
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcyI           70  GGCGCC               G[GA]^CG[CT]C
AflIII       1178  ACATGT               A^C[GA][CT]GT
AocI          334  CCTCAGG              CC^T[ACGT]AGG
BalI          629  TGGCCA               TGG^CCA
BbeI           70  GGCGCC               GGCGC^C
BbsI          123  GAAGAC               GAAGAC(2/6)
BglI           73  GCCCCCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          349  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      349  GCTCAGC              GC^T[ACGT]AGC
BpuAI         123  GAAGAC               GAAGAC(2/6)
BsaHI          70  GGCGCC               G[GA]^CG[CT]C
BsiEI          80  CGGCCG               CG[GA][CT]^CG
BsmI         1127  GAATGC               GAATGC(1/-1)
Bsp1407I      824  TGTACA               T^GTACA
BspLU11I     1178  ACATGT               A^CATGT
BspMI         466  GCAGGT               ACCTGC(4/8)
BsrGI         824  TGTACA               T^GTACA
BstXI        1016  CCAGCACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        334  CCTCAGG              CC^T[ACGT]AGG
CelII         349  GCTCAGC              GC^T[ACGT]AGC
DraI          205  TTTAAA               TTT^AAA
DraIII       1517  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          461  GACTTGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           80  CGGCCG               C^GGCCG
EarI         1488  CTCTTC               CTCTTC(1/4)
EclXI          80  CGGCCG               C^GGCCG
Eco52I         80  CGGCCG               C^GGCCG
Eco81I        334  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1093  CCTGGAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1723  GATATC               GAT^ATC
FauI           76  CCCGC                CCCGC(4/6)
Hin1I          70  GGCGCC               G[GA]^CG[CT]C
HincII        926  GTCAAC               GT[CT]^[GA]AC
HindII        926  GTCAAC               GT[CT]^[GA]AC
KasI           70  GGCGCC               G^GCGCC
Ksp632I      1488  CTCTTC               CTCTTC(1/4)
MluNI         629  TGGCCA               TGG^CCA
MscI          629  TGGCCA               TGG^CCA
NarI           70  GGCGCC               GG^CGCC
NcoI          967  CCATGG               C^CATGG
NdeI          679  CATATG               CA^TATG
NotI           79  GCGGCCGC             GC^GGCCGC
PvuII         365  CAGCTG               CAG^CTG
SapI         1487  GCTCTTC              GCTCTTC(1/4)
SfoI           70  GGCGCC               GGC^GCC
SmaI         1431  CCCGGG               CCC^GGG
SmlI          270  CTTGAG               C^T[CT][GA]AG
SphI          590  GCATGC               GCATG^C
SspBI         824  TGTACA               T^GTACA
XmaCI        1431  CCCGGG               C^CCGGG
XmaI         1431  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII          78  CGCG                 CG^CG
               93  CGCG                
AclI          200  AACGTT               AA^CGTT
              711  AACGTT              
AvaI          157  CCCGAG               C^[CT]CG[GA]G
             1431  CCCGGG              
BanI           70  GGCGCC               G^G[CT][GA]CC
             1089  GGTGCC              
BbvCI        1282  CCTCAGC              CCTCAGC(-5/-2)
             1612  CCTCAGC             
BsgI          857  CTGCAC               GTGCAG(16/14)
             1241  CTGCAC              
BsmFI         459  GGGAC                GGGAC(10/14)
             1535  GTCCC               
BsoBI         157  CCCGAG               C^[CT]CG[GA]G
             1431  CCCGGG              
BspHI         107  TCATGA               T^CATGA
              288  TCATGA              
BstUI          78  CGCG                 CG^CG
               93  CGCG                
BstYI         951  AGATCC               [GA]^GATC[CT]
             1504  GGATCT              
EcoRI         145  GAATTC               G^AATTC
              284  GAATTC              
HaeII          46  AGCGCC               [GA]GCGC^[CT]
               70  GGCGCC              
KspI           77  CCGCGG               CCGC^GG
               92  CCGCGG              
MaeII         201  ACGT                 A^CGT
              712  ACGT                
MflI          951  AGATCC               [GA]^GATC[CT]
             1504  GGATCT              
MvnI           78  CGCG                 CG^CG
               93  CGCG                
PflMI         561  CCAGATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              962  CCAATCCATGG         
PleI          273  GAGTC                GAGTC(4/5)
              516  GAGTC               
Psp1406I      200  AACGTT               AA^CGTT
              711  AACGTT              
RcaI          107  TCATGA               T^CATGA
              288  TCATGA              
SacII          77  CCGCGG               CCGC^GG
               92  CCGCGG              
ScaI         1042  AGTACT               AGT^ACT
             1069  AGTACT              
SfcI          511  CTACAG               C^T[GA][CT]AG
             1618  CTACAG              
StuI          331  AGGCCT               AGG^CCT
             1382  AGGCCT              
Tsp45I        115  GTCAC                ^GT[GC]AC
             1215  GTCAC               
Van91I        561  CCAGATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              962  CCAATCCATGG         
XhoII         951  AGATCC               [GA]^GATC[CT]
             1504  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       
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