Restriction map for pF1KB9906
 (Linear) (Six-base) MAPSORT of: KIBB9906.seq  Check: 3756  from: 1  to: 1767



KIBB9906.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AclI AA'CG_TT

Cuts at:      0     201     712    1767 
   Size:        201     511    1055

  Fragments arranged by size: 

                1055     511     201
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1573    1767 
   Size:       1573     194
 
AflIII A'CryG_T

Cuts at:      0    1178    1767 
   Size:       1178     589
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     287     319    1767 
   Size:        287      32    1448

  Fragments arranged by size: 

                1448     287      32
 
ApoI r'AATT_y

Cuts at:      0     145     248     284    1083    1767 
   Size:        145     103      36     799     684

  Fragments arranged by size: 

                 799     684     145     103      36
 
AvaI C'yCGr_G

Cuts at:      0     157    1431    1767 
   Size:        157    1274     336

  Fragments arranged by size: 

                1274     336     157
 
BanI G'GyrC_C

Cuts at:      0      70    1089    1767 
   Size:         70    1019     678

  Fragments arranged by size: 

                1019     678      70
 
BanII G_rGCy'C

Cuts at:      0      37     101     975    1756    1767 
   Size:         37      64     874     781      11

  Fragments arranged by size: 

                 874     781      64      37      11
 
BbeI G_GCGC'C

Cuts at:      0      74    1767 
   Size:         74    1693
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     130    1767 
   Size:        130    1637
 
BbvCI CC'TCA_GC

Cuts at:      0    1283    1613    1767 
   Size:       1283     330     154

  Fragments arranged by size: 

                1283     330     154
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79    1767 
   Size:         79    1688
 
BlpI GC'TnA_GC

Cuts at:      0     350    1767 
   Size:        350    1417
 
Bme1580I G_kGCm'C

Cuts at:      0     158    1767 
   Size:        158    1609
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1759    1767 
   Size:       1759       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     183    1767 
   Size:        183    1584
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1115    1502    1622    1767 
   Size:       1115     387     120     145

  Fragments arranged by size: 

                1115     387     145     120
 
Bpu10I CC'TnA_GC

Cuts at:      0    1283    1613    1767 
   Size:       1283     330     154

  Fragments arranged by size: 

                1283     330     154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291    1767 
   Size:        291    1476
 
BsaHI Gr'CG_yC

Cuts at:      0      71    1767 
   Size:         71    1696
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1295    1325    1767 
   Size:       1295      30     442

  Fragments arranged by size: 

                1295     442      30
 
BseYI C'CCAG_C

Cuts at:      0    1757    1767 
   Size:       1757      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     842    1226    1767 
   Size:        842     384     541

  Fragments arranged by size: 

                 842     541     384
 
BsiEI CG_ry'CG

Cuts at:      0      83    1767 
   Size:         83    1684
 
BsmI GAATG_Cn'

Cuts at:      0    1133    1767 
   Size:       1133     634
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     101     158     975    1756    1767 
   Size:         37      64      57     817     781      11

  Fragments arranged by size: 

                 817     781      64      57      37      11
 
BspHI T'CATG_A

Cuts at:      0     107     288    1767 
   Size:        107     181    1479

  Fragments arranged by size: 

                1479     181     107
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     457    1767 
   Size:        457    1310
 
BsrDI GCAATG_nn'

Cuts at:      0     381     743    1364    1382    1673    1767 
   Size:        381     362     621      18     291      94

  Fragments arranged by size: 

                 621     381     362     291      94      18
 
BsrGI T'GTAC_A

Cuts at:      0     824    1767 
   Size:        824     943
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1023    1767 
   Size:       1023     744
 
BstYI r'GATC_y

Cuts at:      0     951    1504    1767 
   Size:        951     553     263

  Fragments arranged by size: 

                 951     553     263
 
Bsu36I CC'TnA_GG

Cuts at:      0     335    1767 
   Size:        335    1432
 
BtgI C'CryG_G

Cuts at:      0      77      92     967    1767 
   Size:         77      15     875     800

  Fragments arranged by size: 

                 875     800      77      15
 
BtsI GCAGTG_nn'

Cuts at:      0    1010    1767 
   Size:       1010     757
 
DraI TTT'AAA

Cuts at:      0     207    1767 
   Size:        207    1560
 
DraIII CAC_nnn'GTG

Cuts at:      0    1522    1767 
   Size:       1522     245
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     467    1767 
   Size:        467    1300
 
EaeI y'GGCC_r

Cuts at:      0       2      80     629    1767 
   Size:          2      78     549    1138

  Fragments arranged by size: 

                1138     549      78       2
 
EagI C'GGCC_G

Cuts at:      0      80    1767 
   Size:         80    1687
 
EarI CTCTTCn'nnn_

Cuts at:      0    1494    1767 
   Size:       1494     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1115    1502    1573    1622    1767 
   Size:       1115     387      71      49     145

  Fragments arranged by size: 

                1115     387     145      71      49
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1097    1767 
   Size:       1097     670
 
EcoRI G'AATT_C

Cuts at:      0     145     284    1767 
   Size:        145     139    1483

  Fragments arranged by size: 

                1483     145     139
 
EcoRV GAT'ATC

Cuts at:      0    1725    1767 
   Size:       1725      42
 
HaeII r_GCGC'y

Cuts at:      0      50      74    1767 
   Size:         50      24    1693

  Fragments arranged by size: 

                1693      50      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1295    1327    1336    1368    1709    1741    1767 
   Size:       1295      32       9      32     341      32      26

  Fragments arranged by size: 

                1295     341      32      32      32      26       9
 
HincII GTy'rAC

Cuts at:      0     928    1767 
   Size:        928     839
 
KasI G'GCGC_C

Cuts at:      0      70    1767 
   Size:         70    1697
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     695    1350    1767 
   Size:        695     655     417

  Fragments arranged by size: 

                 695     655     417
 
MscI TGG'CCA

Cuts at:      0     631    1767 
   Size:        631    1136
 
MspA1I CmG'CkG

Cuts at:      0      79      85      94     367    1007    1767 
   Size:         79       6       9     273     640     760

  Fragments arranged by size: 

                 760     640     273      79       9       6
 
NarI GG'CG_CC

Cuts at:      0      71    1767 
   Size:         71    1696
 
NcoI C'CATG_G

Cuts at:      0     967    1767 
   Size:        967     800
 
NdeI CA'TA_TG

Cuts at:      0     680    1767 
   Size:        680    1087
 
NotI GC'GGCC_GC

Cuts at:      0      80    1767 
   Size:         80    1687
 
NspI r_CATG'y

Cuts at:      0     594     825    1182    1767 
   Size:        594     231     357     585

  Fragments arranged by size: 

                 594     585     357     231
 
PasI CC'CwG_GG

Cuts at:      0     796    1444    1767 
   Size:        796     648     323

  Fragments arranged by size: 

                 796     648     323
 
PciI A'CATG_T

Cuts at:      0    1178    1767 
   Size:       1178     589
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     567     968    1767 
   Size:        567     401     799

  Fragments arranged by size: 

                 799     567     401
 
PvuII CAG'CTG

Cuts at:      0     367    1767 
   Size:        367    1400
 
SacII CC_GC'GG

Cuts at:      0      80      95    1767 
   Size:         80      15    1672

  Fragments arranged by size: 

                1672      80      15
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1494    1767 
   Size:       1494     273
 
ScaI AGT'ACT

Cuts at:      0    1044    1071    1767 
   Size:       1044      27     696

  Fragments arranged by size: 

                1044     696      27
 
SfcI C'TryA_G

Cuts at:      0     511    1618    1767 
   Size:        511    1107     149

  Fragments arranged by size: 

                1107     511     149
 
SfoI GGC'GCC

Cuts at:      0      72    1767 
   Size:         72    1695
 
SmaI CCC'GGG

Cuts at:      0    1433    1767 
   Size:       1433     334
 
SmlI C'TyrA_G

Cuts at:      0     270    1767 
   Size:        270    1497
 
SphI G_CATG'C

Cuts at:      0     594    1767 
   Size:        594    1173
 
StuI AGG'CCT

Cuts at:      0     333    1384    1767 
   Size:        333    1051     383

  Fragments arranged by size: 

                1051     383     333
 
StyI C'CwwG_G

Cuts at:      0     453     893     967    1767 
   Size:        453     440      74     800

  Fragments arranged by size: 

                 800     453     440      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     782    1015    1767 
   Size:        782     233     752

  Fragments arranged by size: 

                 782     752     233
 
TatI w'GTAC_w

Cuts at:      0     824    1042    1069    1767 
   Size:        824     218      27     698

  Fragments arranged by size: 

                 824     698     218      27
 
XmaI C'CCGG_G

Cuts at:      0    1431    1767 
   Size:       1431     336
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AloI     ApoI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  Eco57MI    EcoNI    EcoRI    EcoRV    HaeII    Hin4I   HincII
     KasI     MmeI     MscI   MspA1I     NarI     NcoI     NdeI     NotI
     NspI     PasI     PciI    PflMI    PvuII    SacII     SapI     ScaI
     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaWI    BseRI  BsiHKAI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     EciI  EcoICRI
 EcoO109I     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MslI     NaeI   NgoMIV     NheI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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