Restriction map for pF1KB6994
 (Linear) (Six-base) MAPSORT of: KIBB6994.seq  Check: 7480  from: 1  to: 759



KIBB6994.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      48     759 
   Size:         48     711
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     759 
   Size:        320     439
 
AlwNI CAG_nnn'CTG

Cuts at:      0     609     759 
   Size:        609     150
 
ApoI r'AATT_y

Cuts at:      0     280     393     612     710     759 
   Size:        280     113     219      98      49

  Fragments arranged by size: 

                 280     219     113      98      49
 
BamHI G'GATC_C

Cuts at:      0     543     759 
   Size:        543     216
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     494     759 
   Size:        494     265
 
BbvCI CC'TCA_GC

Cuts at:      0     235     759 
   Size:        235     524
 
BclI T'GATC_A

Cuts at:      0     309     759 
   Size:        309     450
 
BglII A'GATC_T

Cuts at:      0     557     759 
   Size:        557     202
 
BlpI GC'TnA_GC

Cuts at:      0      97     759 
   Size:         97     662
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     241     423     759 
   Size:        173      68     182     336

  Fragments arranged by size: 

                 336     182     173      68
 
Bpu10I CC'TnA_GC

Cuts at:      0     235     759 
   Size:        235     524
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     397     759 
   Size:        397     362
 
BsaWI w'CCGG_w

Cuts at:      0     657     759 
   Size:        657     102
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     759 
   Size:        112     647
 
BseYI C'CCAG_C

Cuts at:      0      23     759 
   Size:         23     736
 
BsmI GAATG_Cn'

Cuts at:      0     531     756     759 
   Size:        531     225       3

  Fragments arranged by size: 

                 531     225       3
 
BspHI T'CATG_A

Cuts at:      0     312     759 
   Size:        312     447
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      48     239     759 
   Size:         48     191     520

  Fragments arranged by size: 

                 520     191      48
 
BsrFI r'CCGG_y

Cuts at:      0      92     759 
   Size:         92     667
 
BssHII G'CGCG_C

Cuts at:      0     112     759 
   Size:        112     647
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     345     759 
   Size:        345     414
 
BstYI r'GATC_y

Cuts at:      0     543     557     759 
   Size:        543      14     202

  Fragments arranged by size: 

                 543     202      14
 
BtgI C'CryG_G

Cuts at:      0      44     179     759 
   Size:         44     135     580

  Fragments arranged by size: 

                 580     135      44
 
BtsI GCAGTG_nn'

Cuts at:      0      94     725     759 
   Size:         94     631      34

  Fragments arranged by size: 

                 631      94      34
 
EaeI y'GGCC_r

Cuts at:      0      47     164     182     759 
   Size:         47     117      18     577

  Fragments arranged by size: 

                 577     117      47      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     759 
   Size:        296     463
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     241     320     423     759 
   Size:        173      68      79     103     336

  Fragments arranged by size: 

                 336     173     103      79      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     606     759 
   Size:        606     153
 
EcoRI G'AATT_C

Cuts at:      0     710     759 
   Size:        710      49
 
EcoRV GAT'ATC

Cuts at:      0     321     759 
   Size:        321     438
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     729     759 
   Size:        729      30
 
HindIII A'AGCT_T

Cuts at:      0     532     759 
   Size:        532     227
 
MscI TGG'CCA

Cuts at:      0     166     184     759 
   Size:        166      18     575

  Fragments arranged by size: 

                 575     166      18
 
MspA1I CmG'CkG

Cuts at:      0      46      67     759 
   Size:         46      21     692

  Fragments arranged by size: 

                 692      46      21
 
NaeI GCC'GGC

Cuts at:      0      94     759 
   Size:         94     665
 
NgoMIV G'CCGG_C

Cuts at:      0      92     759 
   Size:         92     667
 
NspI r_CATG'y

Cuts at:      0     105     255     579     759 
   Size:        105     150     324     180

  Fragments arranged by size: 

                 324     180     150     105
 
PasI CC'CwG_GG

Cuts at:      0      10     759 
   Size:         10     749
 
PfoI T'CCnGG_A

Cuts at:      0     146     759 
   Size:        146     613
 
PsiI TTA'TAA

Cuts at:      0     668     759 
   Size:        668      91
 
PstI C_TGCA'G

Cuts at:      0      89     759 
   Size:         89     670
 
SacII CC_GC'GG

Cuts at:      0      47     759 
   Size:         47     712
 
SexAI A'CCwGG_T

Cuts at:      0     503     759 
   Size:        503     256
 
SfcI C'TryA_G

Cuts at:      0      85     759 
   Size:         85     674
 
StuI AGG'CCT

Cuts at:      0     352     759 
   Size:        352     407
 
XbaI T'CTAG_A

Cuts at:      0     276     564     759 
   Size:        276     288     195

  Fragments arranged by size: 

                 288     276     195
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI    BamHI     BbsI    BbvCI     BclI
    BglII     BlpI     BpmI   Bpu10I     BsaI    BsaWI    BseRI    BseYI
     BsmI    BspHI    BspMI    BsrFI   BssHII    BstXI    BstYI     BtgI
     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I
  HindIII     MscI   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI
     PsiI     PstI    SacII    SexAI     SfcI     StuI     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BcgI
    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI
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