Restriction map for pF1KB6989
 (Linear) (Six-base) MAPSORT of: KIBB6989.seq  Check: 4544  from: 1  to: 657



KIBB6989.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     159     657 
   Size:        159     498
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     652     657 
   Size:        652       5
 
AlwNI CAG_nnn'CTG

Cuts at:      0     208     657 
   Size:        208     449
 
ApaLI G'TGCA_C

Cuts at:      0     188     657 
   Size:        188     469
 
ApoI r'AATT_y

Cuts at:      0     643     657 
   Size:        643      14
 
AvaI C'yCGr_G

Cuts at:      0      35     113     502     657 
   Size:         35      78     389     155

  Fragments arranged by size: 

                 389     155      78      35
 
BanII G_rGCy'C

Cuts at:      0     254     657 
   Size:        254     403
 
BglI GCCn_nnn'nGGC

Cuts at:      0     232     557     657 
   Size:        232     325     100

  Fragments arranged by size: 

                 325     232     100
 
Bme1580I G_kGCm'C

Cuts at:      0     192     657 
   Size:        192     465
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     289     657 
   Size:        268      21     368

  Fragments arranged by size: 

                 368     268      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     546     657 
   Size:        546     111
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     374     404     657 
   Size:        374      30     253

  Fragments arranged by size: 

                 374     253      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     520     657 
   Size:        520     137
 
BseYI C'CCAG_C

Cuts at:      0     365     657 
   Size:        365     292
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     329     531     657 
   Size:        154     175     202     126

  Fragments arranged by size: 

                 202     175     154     126
 
BsiHKAI G_wGCw'C

Cuts at:      0      18     192     254     657 
   Size:         18     174      62     403

  Fragments arranged by size: 

                 403     174      62      18
 
BsmI GAATG_Cn'

Cuts at:      0     416     657 
   Size:        416     241
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     192     254     657 
   Size:         18     174      62     403

  Fragments arranged by size: 

                 403     174      62      18
 
BsrDI GCAATG_nn'

Cuts at:      0     636     657 
   Size:        636      21
 
BsrFI r'CCGG_y

Cuts at:      0     601     657 
   Size:        601      56
 
BssSI C'ACGA_G

Cuts at:      0     172     657 
   Size:        172     485
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      69     657 
   Size:         69     588
 
BtgI C'CryG_G

Cuts at:      0     221     255     657 
   Size:        221      34     402

  Fragments arranged by size: 

                 402     221      34
 
BtsI GCAGTG_nn'

Cuts at:      0      80     657 
   Size:         80     577
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     269     304     657 
   Size:        269      35     353

  Fragments arranged by size: 

                 353     269      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     616     657 
   Size:        616      41
 
EaeI y'GGCC_r

Cuts at:      0     224     233     542     657 
   Size:        224       9     309     115

  Fragments arranged by size: 

                 309     224     115       9
 
EcoICRI GAG'CTC

Cuts at:      0     252     657 
   Size:        252     405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     289     657 
   Size:        268      21     368

  Fragments arranged by size: 

                 368     268      21
 
HaeII r_GCGC'y

Cuts at:      0     519     657 
   Size:        519     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177     325     357     657 
   Size:        145      32     148      32     300

  Fragments arranged by size: 

                 300     148     145      32      32
 
MscI TGG'CCA

Cuts at:      0     544     657 
   Size:        544     113
 
MslI CAynn'nnrTG

Cuts at:      0      67      73     657 
   Size:         67       6     584

  Fragments arranged by size: 

                 584      67       6
 
MspA1I CmG'CkG

Cuts at:      0     168     205     246     598     657 
   Size:        168      37      41     352      59

  Fragments arranged by size: 

                 352     168      59      41      37
 
NcoI C'CATG_G

Cuts at:      0     255     657 
   Size:        255     402
 
NspI r_CATG'y

Cuts at:      0     498     657 
   Size:        498     159
 
PasI CC'CwG_GG

Cuts at:      0     402     657 
   Size:        402     255
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222     657 
   Size:        222     435
 
PstI C_TGCA'G

Cuts at:      0     126     657 
   Size:        126     531
 
PvuII CAG'CTG

Cuts at:      0     205     246     598     657 
   Size:        205      41     352      59

  Fragments arranged by size: 

                 352     205      59      41
 
SacI G_AGCT'C

Cuts at:      0     254     657 
   Size:        254     403
 
SexAI A'CCwGG_T

Cuts at:      0     581     657 
   Size:        581      76
 
SfcI C'TryA_G

Cuts at:      0     122     657 
   Size:        122     535
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     232     657 
   Size:        232     425
 
SmaI CCC'GGG

Cuts at:      0      37     657 
   Size:         37     620
 
SmlI C'TyrA_G

Cuts at:      0     561     657 
   Size:        561      96
 
StyI C'CwwG_G

Cuts at:      0     255     657 
   Size:        255     402
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      72     657 
   Size:         72     585
 
XmaI C'CCGG_G

Cuts at:      0      35     657 
   Size:         35     622
 

 Enzymes that do cut:

     AhdI     AlfI    AlwNI    ApaLI     ApoI     AvaI    BanII     BglI
 Bme1580I     BpmI    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BsrFI    BssSI    BstXI     BtgI     BtsI
    CspCI     DrdI     EaeI  EcoICRI  Eco57MI    HaeII    Hin4I     MscI
     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PstI    PvuII
     SacI    SexAI     SfcI     SfiI     SmaI     SmlI     StyI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AloI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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