Restriction map for pF1KB6984
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AfaI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HapII       HincII      HindII      HindIII     HpaI        
HpaII       KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspI        MunI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII         364  CGCG                 CG^CG
AcyI          451  GGCGCC               G[GA]^CG[CT]C
AflIII        545  ACATGT               A^C[GA][CT]GT
AlwI           15  GATCC                GGATC(4/5)
BanI          451  GGCGCC               G^G[CT][GA]CC
BanII         444  GAGCTC               G[GA]GC[CT]^C
BbeI          451  GGCGCC               GGCGC^C
BbrPI         344  CACGTG               CAC^GTG
BbsI          303  GAAGAC               GAAGAC(2/6)
BlpI          495  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      495  GCTGAGC              GC^T[ACGT]AGC
BpuAI         303  GAAGAC               GAAGAC(2/6)
BsaAI         344  CACGTG               [CT]AC^GT[GA]
BsaHI         451  GGCGCC               G[GA]^CG[CT]C
BsgI          347  GTGCAG               GTGCAG(16/14)
BspLU11I      545  ACATGT               A^CATGT
BsrDI         617  GCAATG               GCAATG(2/0)
BstAPI        407  GCACGAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         364  CGCG                 CG^CG
BstXI         135  CCACTTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         495  GCTGAGC              GC^T[ACGT]AGC
CfoI          452  GCGC                 GCG^C
Cfr13I        604  GGCCC                G^G[ACGT]CC
DraIII        408  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
HaeII         451  GGCGCC               [GA]GCGC^[CT]
HgaI          484  GACGC                GACGC(5/10)
HhaI          452  GCGC                 GCG^C
Hin1I         451  GGCGCC               G[GA]^CG[CT]C
HinP1I        452  GCGC                 G^CGC
KasI          451  GGCGCC               G^GCGCC
MaeII         345  ACGT                 A^CGT
MspA1I         32  CAGCTG               C[AC]G^C[GT]G
MvnI          364  CGCG                 CG^CG
NarI          451  GGCGCC               GG^CGCC
NspI          545  ACATGT               [GA]CATG^[CT]
PflMI         397  CCAGCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         344  CACGTG               CAC^GTG
PmlI          344  CACGTG               CAC^GTG
PstI          472  CTGCAG               CTGCA^G
PvuII          32  CAGCTG               CAG^CTG
SacI          444  GAGCTC               GAGCT^C
Sau96I        604  GGCCC                G^G[ACGT]CC
SexAI         110  ACCAGGT              A^CC[AT]GGT
SfaNI         203  GCATC                GCATC(5/9)
SfcI          472  CTGCAG               C^T[GA][CT]AG
SfoI          451  GGCGCC               GGC^GCC
SmlI          565  CTTGAG               C^T[CT][GA]AG
TaqI          233  TCGA                 T^CGA
TthHB8I       233  TCGA                 T^CGA
Van91I        397  CCAGCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          135  CCACTTCTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          682  GTCTAC               GT^[AC][GT]AC
              694  GTCTAC              
AcsI           96  GAATTC               [GA]^AATT[CT]
              123  GAATTC              
AlwNI         398  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
              475  CAGTGCCTG           
ApoI           96  GAATTC               [GA]^AATT[CT]
              123  GAATTC              
AspHI         223  GAGCAC               G[AT]GC[AT]^C
              444  GAGCTC              
BseRI         339  CTCCTC               GAGGAG(10/8)
               91  GAGGAG              
BsiHKAI       223  GAGCAC               G[AT]GC[AT]^C
              444  GAGCTC              
BsmAI         213  GAGAC                GTCTC(1/5)
              640  GTCTC               
BsrI          216  ACTGG                ACTGG(1/-1)
              403  ACTGG               
BstF5I        204  CATCC                GGATG(2/0)
              279  CATCC               
DdeI          496  CTGAG                C^T[ACGT]AG
              506  CTAAG               
EcoRI          96  GAATTC               G^AATTC
              123  GAATTC              
EcoT14I       630  CCAAGG               C^C[AT][AT]GG
              689  CCAAGG              
FauI          200  CCCGC                CCCGC(4/6)
              362  CCCGC               
FokI          204  CATCC                GGATG(9/13)
              279  CATCC               
HaeIII        184  GGCC                 GG^CC
              604  GGCC                
MboII         303  GAAGA                GAAGA(8/7)
              666  GAAGA               
MseI          328  TTAA                 T^TAA
              716  TTAA                
MslI          136  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              204  CATCCAGGTG          
NlaIV         148  GGCTCC               GG[ACGT]^[ACGT]CC
              451  GGCGCC              
PleI          306  GACTC                GAGTC(4/5)
              680  GAGTC               
StyI          630  CCAAGG               C^C[AT][AT]GG
              689  CCAAGG              
Tru9I         328  TTAA                 T^TAA
              716  TTAA                
Tsp509I        97  AATT                 ^AATT
              124  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AluI        BbvI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstNI       Cac8I       DpnII       EcoRII      
Fnu4HI      HinfI       HphI        ItaI        MboI        
MnlI        MvaI        MwoI        NdeII       NlaIII      
Sau3AI      ScrFI       TfiI        TseI        TspRI       
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