Restriction map for pF1KB6983
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       ApoI        
AscI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BseRI       BsgI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        HphI        KpnI        
MaeI        MamI        MfeI        MluI        MslI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        Tsp509I     Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        569  TCCGGA               T^CCGGA
AfaI          288  GTAC                 GT^AC
AlwNI         373  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           58  GGGCCC               GGGCC^C
AseI          328  ATTAAT               AT^TAAT
AsnI          328  ATTAAT               AT^TAAT
AspHI         589  GAGCTC               G[AT]GC[AT]^C
BalI          300  TGGCCA               TGG^CCA
BanI          481  GGCGCC               G^G[CT][GA]CC
BbeI          481  GGCGCC               GGCGC^C
BbrPI         493  CACGTG               CAC^GTG
BpmI          436  CTGGAG               CTGGAG(16/14)
BsaAI         493  CACGTG               [CT]AC^GT[GA]
BsaWI         569  TCCGGA               [AT]^CCGG[AT]
BseAI         569  TCCGGA               T^CCGGA
BsiHKAI       589  GAGCTC               G[AT]GC[AT]^C
BsmBI         526  CGTCTC               CGTCTC(1/5)
BspEI         569  TCCGGA               T^CCGGA
BsrFI         335  ACCGGC               [GA]^CCGG[CT]
BssHII        128  GCGCGC               G^CGCGC
Cfr10I        335  ACCGGC               [GA]^CCGG[CT]
DraIII        490  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
KasI          481  GGCGCC               G^GCGCC
KspI          251  CCGCGG               CCGC^GG
MaeIII        441  GTCAC                ^GT[ACGT]AC
MluNI         300  TGGCCA               TGG^CCA
MroI          569  TCCGGA               T^CCGGA
MscI          300  TGGCCA               TGG^CCA
MseI          329  TTAA                 T^TAA
NarI          481  GGCGCC               GG^CGCC
NspI          290  ACATGC               [GA]CATG^[CT]
PflMI         489  CCACCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         493  CACGTG               CAC^GTG
PmlI          493  CACGTG               CAC^GTG
PshBI         328  ATTAAT               AT^TAAT
PstI          207  CTGCAG               CTGCA^G
PvuII         553  CAGCTG               CAG^CTG
RsaI          288  GTAC                 GT^AC
SacI          589  GAGCTC               GAGCT^C
SacII         251  CCGCGG               CCGC^GG
SapI          363  GCTCTTC              GCTCTTC(1/4)
SfaNI         221  GCATC                GCATC(5/9)
SfcI          207  CTGCAG               C^T[GA][CT]AG
SfoI          481  GGCGCC               GGC^GCC
StuI          509  AGGCCT               AGG^CCT
TfiI          375  GATTC                G^A[AT]TC
Tru9I         329  TTAA                 T^TAA
Tsp45I        441  GTCAC                ^GT[GC]AC
Van91I        489  CCACCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI           15  GGCGTC               G[GA]^CG[CT]C
              481  GGCGCC              
AvaI           41  CCCGGG               C^[CT]CG[GA]G
               61  CCCGGG              
BglII         203  AGATCT               A^GATCT
              432  AGATCT              
BsaHI          15  GGCGTC               G[GA]^CG[CT]C
              481  GGCGCC              
BsmAI          82  GAGAC                GTCTC(1/5)
              527  GTCTC               
BsoBI          41  CCCGGG               C^[CT]CG[GA]G
               61  CCCGGG              
BstYI         203  AGATCT               [GA]^GATC[CT]
              432  AGATCT              
DsaI          251  CCGCGG               C^C[GA][CT]GG
              303  CCACGG              
EaeI          300  TGGCCA               [CT]^GGCC[GA]
              515  CGGCCA              
EarI          364  CTCTTC               CTCTTC(1/4)
              262  GAAGAG              
HaeII         446  AGCGCC               [GA]GCGC^[CT]
              481  GGCGCC              
Hin1I          15  GGCGTC               G[GA]^CG[CT]C
              481  GGCGCC              
Ksp632I       364  CTCTTC               CTCTTC(1/4)
              262  GAAGAG              
MaeII         494  ACGT                 A^CGT
              533  ACGT                
MflI          203  AGATCT               [GA]^GATC[CT]
              432  AGATCT              
NlaIII        276  CATG                 CATG^
              291  CATG                
PleI          190  GACTC                GAGTC(4/5)
              439  GAGTC               
SmaI           41  CCCGGG               CCC^GGG
               61  CCCGGG              
TspRI         210  CAGTG                CA[GC]TG[ACGT][ACGT]^
              609  CAGTG               
XhoII         203  AGATCT               [GA]^GATC[CT]
              432  AGATCT              
XmaCI          41  CCCGGG               C^CCGGG
               61  CCCGGG              
XmaI           41  CCCGGG               C^CCGGG
               61  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       ItaI        MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       PpuMI       Sau3AI      
Sau96I      ScrFI       TseI        
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