Restriction map for pF1KB7810
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbsI        BbvCI       BfrI        
BlnI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspLU11I    
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      Eam1105I    Eco47III    
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FspI        HincII      HindII      HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MslI        MunI        NdeI        NheI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tsp45I      
Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          214  GTCTAC               GT^[AC][GT]AC
AcsI          456  AAATTC               [GA]^AATT[CT]
Alw44I        678  GTGCAC               G^TGCAC
AlwNI        1504  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         678  GTGCAC               G^TGCAC
ApoI          456  AAATTC               [GA]^AATT[CT]
BbeI          169  GGCGCC               GGCGC^C
BcgI         1082  CGAAGAGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         761  GGATAC               GTATCC(6/5)
BglI          473  GCCAGGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1268  AGATCT               A^GATCT
BlpI          964  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      964  GCTGAGC              GC^T[ACGT]AGC
BsaI         1168  GGTCTC               GGTCTC(1/5)
BsmBI        1684  GAGACG               CGTCTC(1/5)
BsmFI         924  GTCCC                GGGAC(10/14)
BspHI         887  TCATGA               T^CATGA
BsrDI         563  GCAATG               GCAATG(2/0)
BstXI         272  CCAACGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         964  GCTGAGC              GC^T[ACGT]AGC
DrdI          937  GACCAGATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         114  CCTGGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI          429  GGCCGGCC             GGCCGG^CC
HindIII      1016  AAGCTT               A^AGCTT
HphI          832  GGTGA                GGTGA(8/7)
KasI          169  GGCGCC               G^GCGCC
KspI          191  CCGCGG               CCGC^GG
MaeII        1595  ACGT                 A^CGT
MaeIII       1553  GTAAC                ^GT[ACGT]AC
MseI         1361  TTAA                 T^TAA
NarI          169  GGCGCC               GG^CGCC
NcoI         1184  CCATGG               C^CATGG
NotI          193  GCGGCCGC             GC^GGCCGC
PvuII         346  CAGCTG               CAG^CTG
RcaI          887  TCATGA               T^CATGA
SacII         191  CCGCGG               CCGC^GG
SapI          702  GAAGAGC              GCTCTTC(1/4)
SfoI          169  GGCGCC               GGC^GCC
SmaI          244  CCCGGG               CCC^GGG
SmlI           91  CTCAAG               C^T[CT][GA]AG
Tru9I        1361  TTAA                 T^TAA
XbaI         1134  TCTAGA               T^CTAGA
XcmI          807  CCAGAGAGATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         244  CCCGGG               C^CCGGG
XmaI          244  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AspHI         678  GTGCAC               G[AT]GC[AT]^C
             1189  GAGCAC              
AvaI          163  CCCGAG               C^[CT]CG[GA]G
              244  CCCGGG              
BsgI          331  CTGCAC               GTGCAG(16/14)
              554  GTGCAG              
BsiEI         194  CGGCCG               CG[GA][CT]^CG
              932  CGGCCG              
BsiHKAI       678  GTGCAC               G[AT]GC[AT]^C
             1189  GAGCAC              
BsmI          442  GCATTC               GAATGC(1/-1)
             1728  GCATTC              
BsoBI         163  CCCGAG               C^[CT]CG[GA]G
              244  CCCGGG              
BsrBI         883  CCGCTC               CCGCTC(-3/-3)
              106  GAGCGG              
BstAPI       1580  GCATGAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1728  GCATTCCTTGC         
EagI          194  CGGCCG               C^GGCCG
              932  CGGCCG              
EclXI         194  CGGCCG               C^GGCCG
              932  CGGCCG              
Eco52I        194  CGGCCG               C^GGCCG
              932  CGGCCG              
HgaI          470  GACGC                GACGC(5/10)
             1633  GACGC               
NspI         1277  ACATGC               [GA]CATG^[CT]
             1647  ACATGC              
TfiI          529  GAATC                G^A[AT]TC
             1314  GAATC               
Tsp509I       457  AATT                 ^AATT
             1363  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaII       BalI        BanI        BanII       
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    Bsp1407I    BspMI       
BsrFI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MaeI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StuI        StyI        TaqI        TseI        
TspRI       TthHB8I     Van91I      XhoII       
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