Restriction map for pF1KB7810
 (Linear) (Six-base) MAPSORT of: KIBB7810.seq  Check: 4142  from: 1  to: 1785



KIBB7810.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     369    1785 
   Size:        369    1416
 
AccI GT'mk_AC

Cuts at:      0     215    1785 
   Size:        215    1570
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     993    1417    1785 
   Size:        560     433     424     368

  Fragments arranged by size: 

                 560     433     424     368
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1509    1785 
   Size:       1509     276
 
ApaLI G'TGCA_C

Cuts at:      0     678    1785 
   Size:        678    1107
 
ApoI r'AATT_y

Cuts at:      0     456    1785 
   Size:        456    1329
 
AvaI C'yCGr_G

Cuts at:      0     163     244    1785 
   Size:        163      81    1541

  Fragments arranged by size: 

                1541     163      81
 
BanI G'GyrC_C

Cuts at:      0     169     381    1089    1599    1785 
   Size:        169     212     708     510     186

  Fragments arranged by size: 

                 708     510     212     186     169
 
BanII G_rGCy'C

Cuts at:      0      74     319     407     971    1717    1785 
   Size:         74     245      88     564     746      68

  Fragments arranged by size: 

                 746     564     245      88      74      68
 
BbeI G_GCGC'C

Cuts at:      0     173    1785 
   Size:        173    1612
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1071    1105    1785 
   Size:       1071      34     680

  Fragments arranged by size: 

                1071     680      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     755    1785 
   Size:        755    1030
 
BclI T'GATC_A

Cuts at:      0     890    1070    1119    1785 
   Size:        890     180      49     666

  Fragments arranged by size: 

                 890     666     180      49
 
BglI GCCn_nnn'nGGC

Cuts at:      0     479    1785 
   Size:        479    1306
 
BglII A'GATC_T

Cuts at:      0    1268    1785 
   Size:       1268     517
 
BlpI GC'TnA_GC

Cuts at:      0     965    1785 
   Size:        965     820
 
Bme1580I G_kGCm'C

Cuts at:      0     386     682    1604    1785 
   Size:        386     296     922     181

  Fragments arranged by size: 

                 922     386     296     181
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1086    1785 
   Size:       1086     699
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     124     415     869    1388    1642    1785 
   Size:         88      36     291     454     519     254     143

  Fragments arranged by size: 

                 519     454     291     254     143      88      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1785 
   Size:         76    1709
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1174    1785 
   Size:       1174     611
 
BsaHI Gr'CG_yC

Cuts at:      0     170     471    1634    1785 
   Size:        170     301    1163     151

  Fragments arranged by size: 

                1163     301     170     151
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1321    1351    1558    1588    1785 
   Size:       1321      30     207      30     197

  Fragments arranged by size: 

                1321     207     197      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     794    1342    1510    1513    1696    1785 
   Size:        794     548     168       3     183      89

  Fragments arranged by size: 

                 794     548     183     168      89       3
 
BseYI C'CCAG_C

Cuts at:      0     406    1514    1785 
   Size:        406    1108     271

  Fragments arranged by size: 

                1108     406     271
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     575    1785 
   Size:        316     259    1210

  Fragments arranged by size: 

                1210     316     259
 
BsiEI CG_ry'CG

Cuts at:      0     197     935    1785 
   Size:        197     738     850

  Fragments arranged by size: 

                 850     738     197
 
BsiHKAI G_wGCw'C

Cuts at:      0     682    1193    1785 
   Size:        682     511     592

  Fragments arranged by size: 

                 682     592     511
 
BsmI GAATG_Cn'

Cuts at:      0     442    1728    1785 
   Size:        442    1286      57

  Fragments arranged by size: 

                1286     442      57
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1678    1785 
   Size:       1678     107
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     319     386     407     682     971    1193    1604 
   Size:         74     245      67      21     275     289     222     411

Cuts at:   1604    1717    1785 
   Size:        113      68

  Fragments arranged by size: 

                 411     289     275     245     222     113      74      68
                  67      21
 
BspHI T'CATG_A

Cuts at:      0     887    1785 
   Size:        887     898
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     201     369    1622    1785 
   Size:        201     168    1253     163

  Fragments arranged by size: 

                1253     201     168     163
 
BsrBI CCG'CTC

Cuts at:      0     108     885    1785 
   Size:        108     777     900

  Fragments arranged by size: 

                 900     777     108
 
BsrDI GCAATG_nn'

Cuts at:      0     570    1785 
   Size:        570    1215
 
BsrFI r'CCGG_y

Cuts at:      0     226     430     438     719    1785 
   Size:        226     204       8     281    1066

  Fragments arranged by size: 

                1066     281     226     204       8
 
BsrGI T'GTAC_A

Cuts at:      0     152    1373    1574    1785 
   Size:        152    1221     201     211

  Fragments arranged by size: 

                1221     211     201     152
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1586    1734    1785 
   Size:       1586     148      51

  Fragments arranged by size: 

                1586     148      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     279    1785 
   Size:        279    1506
 
BstYI r'GATC_y

Cuts at:      0      50      95    1154    1268    1785 
   Size:         50      45    1059     114     517

  Fragments arranged by size: 

                1059     517     114      50      45
 
BtgI C'CryG_G

Cuts at:      0     191     369    1184    1662    1676    1778    1785 
   Size:        191     178     815     478      14     102       7

  Fragments arranged by size: 

                 815     478     191     178     102      14       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1709    1785 
   Size:       1709      76
 
BtsI GCAGTG_nn'

Cuts at:      0     563    1706    1785 
   Size:        563    1143      79

  Fragments arranged by size: 

                1143     563      79
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     943    1785 
   Size:        943     842
 
EaeI y'GGCC_r

Cuts at:      0     194     372     494     874     932    1469    1703    1785 
   Size:        194     178     122     380      58     537     234      82

  Fragments arranged by size: 

                 537     380     234     194     178     122      82      58
            
 
EagI C'GGCC_G

Cuts at:      0     194     932    1785 
   Size:        194     738     853

  Fragments arranged by size: 

                 853     738     194
 
EarI CTCTTCn'nnn_

Cuts at:      0     697     771    1078    1403    1785 
   Size:        697      74     307     325     382

  Fragments arranged by size: 

                 697     382     325     307      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     124     415     560     869     993    1388    1417 
   Size:         88      36     291     145     309     124     395      29

Cuts at:   1417    1642    1785 
   Size:        225     143

  Fragments arranged by size: 

                 395     309     291     225     145     143     124      88
                  36      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     118    1785 
   Size:        118    1667
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     639    1785 
   Size:        639    1146
 
FseI GG_CCGG'CC

Cuts at:      0     434    1785 
   Size:        434    1351
 
HaeII r_GCGC'y

Cuts at:      0     173     807    1182    1785 
   Size:        173     634     375     603

  Fragments arranged by size: 

                 634     603     375     173
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1152    1184    1785 
   Size:       1152      32     601

  Fragments arranged by size: 

                1152     601      32
 
HindIII A'AGCT_T

Cuts at:      0    1016    1785 
   Size:       1016     769
 
KasI G'GCGC_C

Cuts at:      0     169    1785 
   Size:        169    1616
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     938    1785 
   Size:        296     642     847

  Fragments arranged by size: 

                 847     642     296
 
MscI TGG'CCA

Cuts at:      0     496     876    1471    1705    1785 
   Size:        496     380     595     234      80

  Fragments arranged by size: 

                 595     496     380     234      80
 
MspA1I CmG'CkG

Cuts at:      0     193     321     348     410    1506    1785 
   Size:        193     128      27      62    1096     279

  Fragments arranged by size: 

                1096     279     193     128      62      27
 
NaeI GCC'GGC

Cuts at:      0     432     440     721    1785 
   Size:        432       8     281    1064

  Fragments arranged by size: 

                1064     432     281       8
 
NarI GG'CG_CC

Cuts at:      0     170    1785 
   Size:        170    1615
 
NcoI C'CATG_G

Cuts at:      0    1184    1785 
   Size:       1184     601
 
NgoMIV G'CCGG_C

Cuts at:      0     430     438     719    1785 
   Size:        430       8     281    1066

  Fragments arranged by size: 

                1066     430     281       8
 
NotI GC'GGCC_GC

Cuts at:      0     194    1785 
   Size:        194    1591
 
NspI r_CATG'y

Cuts at:      0    1281    1651    1785 
   Size:       1281     370     134

  Fragments arranged by size: 

                1281     370     134
 
PasI CC'CwG_GG

Cuts at:      0     114    1195    1785 
   Size:        114    1081     590

  Fragments arranged by size: 

                1081     590     114
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     872    1467    1509    1785 
   Size:        872     595      42     276

  Fragments arranged by size: 

                 872     595     276      42
 
PfoI T'CCnGG_A

Cuts at:      0      28    1433    1785 
   Size:         28    1405     352

  Fragments arranged by size: 

                1405     352      28
 
PstI C_TGCA'G

Cuts at:      0     365    1322    1493    1785 
   Size:        365     957     171     292

  Fragments arranged by size: 

                 957     365     292     171
 
PvuII CAG'CTG

Cuts at:      0     348    1785 
   Size:        348    1437
 
SacII CC_GC'GG

Cuts at:      0     194    1785 
   Size:        194    1591
 
SapI GCTCTTCn'nnn_

Cuts at:      0     697    1785 
   Size:        697    1088
 
SfcI C'TryA_G

Cuts at:      0     361     447    1318    1489    1785 
   Size:        361      86     871     171     296

  Fragments arranged by size: 

                 871     361     296     171      86
 
SfoI GGC'GCC

Cuts at:      0     171    1785 
   Size:        171    1614
 
SmaI CCC'GGG

Cuts at:      0     246    1785 
   Size:        246    1539
 
SmlI C'TyrA_G

Cuts at:      0      91    1785 
   Size:         91    1694
 
StuI AGG'CCT

Cuts at:      0     619     715    1481    1785 
   Size:        619      96     766     304

  Fragments arranged by size: 

                 766     619     304      96
 
StyI C'CwwG_G

Cuts at:      0      54     536    1184    1472    1785 
   Size:         54     482     648     288     313

  Fragments arranged by size: 

                 648     482     313     288      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     788    1208    1785 
   Size:        788     420     577

  Fragments arranged by size: 

                 788     577     420
 
TatI w'GTAC_w

Cuts at:      0     152    1373    1574    1785 
   Size:        152    1221     201     211

  Fragments arranged by size: 

                1221     211     201     152
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1321    1353    1785 
   Size:       1321      32     432

  Fragments arranged by size: 

                1321     432      32
 
XbaI T'CTAG_A

Cuts at:      0    1134    1785 
   Size:       1134     651
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     814    1785 
   Size:        814     971
 
XmaI C'CCGG_G

Cuts at:      0     244    1785 
   Size:        244    1541
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI
     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI  Eco57MI
    EcoNI     FalI     FseI    HaeII    Hin4I  HindIII     KasI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NspI
     PasI    PflMI     PfoI     PstI    PvuII    SacII     SapI     SfcI
     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI    BfrBI    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaBI    BsaWI    BsiWI    BspEI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FspI    FspAI   HincII
     HpaI     KpnI     MfeI     MluI     MslI     NdeI     NheI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XhoI     XmnI
     ZraI
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