Restriction map for
pF1KB6979
(Linear) (Six-base) MAPSORT of: KIBB6979.seq Check: 2672 from: 1 to: 654
KIBB6979.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 594 654
Size: 64 530 60
Fragments arranged by size:
530 64 60
AflIII A'CryG_T
Cuts at: 0 293 648 654
Size: 293 355 6
Fragments arranged by size:
355 293 6
AleI CACnn'nnGTG
Cuts at: 0 382 591 654
Size: 382 209 63
Fragments arranged by size:
382 209 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 400 654
Size: 376 24 254
Fragments arranged by size:
376 254 24
ApaI G_GGCC'C
Cuts at: 0 38 72 654
Size: 38 34 582
Fragments arranged by size:
582 38 34
ApaLI G'TGCA_C
Cuts at: 0 375 654
Size: 375 279
AvaI C'yCGr_G
Cuts at: 0 127 263 654
Size: 127 136 391
Fragments arranged by size:
391 136 127
BamHI G'GATC_C
Cuts at: 0 452 654
Size: 452 202
BanI G'GyrC_C
Cuts at: 0 123 147 384 593 654
Size: 123 24 237 209 61
Fragments arranged by size:
237 209 123 61 24
BanII G_rGCy'C
Cuts at: 0 38 72 654
Size: 38 34 582
Fragments arranged by size:
582 38 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 67 654
Size: 67 587
BclI T'GATC_A
Cuts at: 0 395 654
Size: 395 259
BlpI GC'TnA_GC
Cuts at: 0 175 654
Size: 175 479
Bme1580I G_kGCm'C
Cuts at: 0 38 72 379 598 654
Size: 38 34 307 219 56
Fragments arranged by size:
307 219 56 38 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 327 654
Size: 327 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 654
Size: 597 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 349 469 654
Size: 172 177 120 185
Fragments arranged by size:
185 177 172 120
BsaAI yAC'GTr
Cuts at: 0 649 654
Size: 649 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 427 457 654
Size: 241 30 156 30 197
Fragments arranged by size:
241 197 156 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 482 545 654
Size: 472 10 63 109
Fragments arranged by size:
472 109 63 10
BsiHKAI G_wGCw'C
Cuts at: 0 365 379 654
Size: 365 14 275
Fragments arranged by size:
365 275 14
Bsp1286I G_dGCh'C
Cuts at: 0 38 72 365 379 598 654
Size: 38 34 293 14 219 56
Fragments arranged by size:
293 219 56 38 34 14
BspHI T'CATG_A
Cuts at: 0 56 654
Size: 56 598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 572 654
Size: 572 82
BsrBI CCG'CTC
Cuts at: 0 438 654
Size: 438 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 558 654
Size: 357 201 96
Fragments arranged by size:
357 201 96
BstYI r'GATC_y
Cuts at: 0 452 654
Size: 452 202
Bsu36I CC'TnA_GG
Cuts at: 0 152 654
Size: 152 502
BtgI C'CryG_G
Cuts at: 0 245 654
Size: 245 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 594 597 654
Size: 64 530 3 57
Fragments arranged by size:
530 64 57 3
EcoO109I rG'GnC_Cy
Cuts at: 0 34 68 69 369 562 654
Size: 34 34 1 300 193 92
Fragments arranged by size:
300 193 92 34 34 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 241 273 654
Size: 168 32 41 32 381
Fragments arranged by size:
381 168 41 32 32
MslI CAynn'nnrTG
Cuts at: 0 382 591 654
Size: 382 209 63
Fragments arranged by size:
382 209 63
MspA1I CmG'CkG
Cuts at: 0 289 654
Size: 289 365
NspI r_CATG'y
Cuts at: 0 297 654
Size: 297 357
PasI CC'CwG_GG
Cuts at: 0 566 654
Size: 566 88
PciI A'CATG_T
Cuts at: 0 293 654
Size: 293 361
PfoI T'CCnGG_A
Cuts at: 0 92 431 654
Size: 92 339 223
Fragments arranged by size:
339 223 92
PpuMI rG'GwC_Cy
Cuts at: 0 562 654
Size: 562 92
PspOMI G'GGCC_C
Cuts at: 0 34 68 654
Size: 34 34 586
Fragments arranged by size:
586 34 34
PstI C_TGCA'G
Cuts at: 0 230 654
Size: 230 424
PvuII CAG'CTG
Cuts at: 0 289 654
Size: 289 365
SanDI GG'GwC_CC
Cuts at: 0 562 654
Size: 562 92
SfcI C'TryA_G
Cuts at: 0 226 654
Size: 226 428
SmlI C'TyrA_G
Cuts at: 0 187 364 484 654
Size: 187 177 120 170
Fragments arranged by size:
187 177 170 120
StyI C'CwwG_G
Cuts at: 0 380 654
Size: 380 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 462 654
Size: 462 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 654
Size: 463 191
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaI ApaLI AvaI BamHI
BanI BanII BbsI BclI BlpI Bme1580I BmrI BpmI
BpuEI BsaAI BsaXI BseRI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BstXI BstYI Bsu36I BtgI Eco57MI EcoO109I Hin4I
MslI MspA1I NspI PasI PciI PfoI PpuMI PspOMI
PstI PvuII SanDI SfcI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI