Restriction map for pF1KB8844
 (Linear) (Six-base) MAPSORT of: KIBB8844.seq  Check: 4878  from: 1  to: 1566



KIBB8844.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     851    1127    1566 
   Size:        851     276     439

  Fragments arranged by size: 

                 851     439     276
 
AatII G_ACGT'C

Cuts at:      0    1058    1566 
   Size:       1058     508
 
AflIII A'CryG_T

Cuts at:      0     962    1340    1566 
   Size:        962     378     226

  Fragments arranged by size: 

                 962     378     226
 
AlwNI CAG_nnn'CTG

Cuts at:      0      93     333     852    1566 
   Size:         93     240     519     714

  Fragments arranged by size: 

                 714     519     240      93
 
ApaI G_GGCC'C

Cuts at:      0    1157    1566 
   Size:       1157     409
 
AvaI C'yCGr_G

Cuts at:      0      51     646    1566 
   Size:         51     595     920

  Fragments arranged by size: 

                 920     595      51
 
BamHI G'GATC_C

Cuts at:      0     893    1566 
   Size:        893     673
 
BanI G'GyrC_C

Cuts at:      0     113     177    1291    1566 
   Size:        113      64    1114     275

  Fragments arranged by size: 

                1114     275     113      64
 
BanII G_rGCy'C

Cuts at:      0     818     890    1106    1157    1500    1566 
   Size:        818      72     216      51     343      66

  Fragments arranged by size: 

                 818     343     216      72      66      51
 
BbeI G_GCGC'C

Cuts at:      0     181    1566 
   Size:        181    1385
 
BbvCI CC'TCA_GC

Cuts at:      0      40    1566 
   Size:         40    1526
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     594     628     783     817    1566 
   Size:        594      34     155      34     749

  Fragments arranged by size: 

                 749     594     155      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     571    1566 
   Size:        571     995
 
BclI T'GATC_A

Cuts at:      0    1505    1566 
   Size:       1505      61
 
BfrBI ATG'CAT

Cuts at:      0    1431    1566 
   Size:       1431     135
 
BglI GCCn_nnn'nGGC

Cuts at:      0     203    1168    1231    1566 
   Size:        203     965      63     335

  Fragments arranged by size: 

                 965     335     203      63
 
Bme1580I G_kGCm'C

Cuts at:      0     118     530     972    1157    1294    1566 
   Size:        118     412     442     185     137     272

  Fragments arranged by size: 

                 442     412     272     185     137     118
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     768    1566 
   Size:        768     798
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1539    1566 
   Size:       1539      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793    1566 
   Size:        793     773
 
Bpu10I CC'TnA_GC

Cuts at:      0      40    1566 
   Size:         40    1526
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     574    1566 
   Size:        544      30     992

  Fragments arranged by size: 

                 992     544      30
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      25     823    1566 
   Size:         25     798     743

  Fragments arranged by size: 

                 798     743      25
 
BsaAI yAC'GTr

Cuts at:      0     275    1341    1566 
   Size:        275    1066     225

  Fragments arranged by size: 

                1066     275     225
 
BsaBI GATnn'nnATC

Cuts at:      0     293    1566 
   Size:        293    1273
 
BsaHI Gr'CG_yC

Cuts at:      0     178     320    1055    1064    1566 
   Size:        178     142     735       9     502

  Fragments arranged by size: 

                 735     502     178     142       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     289     319    1487    1517    1566 
   Size:        289      30    1168      30      49

  Fragments arranged by size: 

                1168     289      49      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57    1566 
   Size:         57    1509
 
BseYI C'CCAG_C

Cuts at:      0      72     106     201    1359    1566 
   Size:         72      34      95    1158     207

  Fragments arranged by size: 

                1158     207      95      72      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     625     688    1288    1566 
   Size:        331     294      63     600     278

  Fragments arranged by size: 

                 600     331     294     278      63
 
BsiEI CG_ry'CG

Cuts at:      0    1130    1566 
   Size:       1130     436
 
BsiHKAI G_wGCw'C

Cuts at:      0     653    1253    1337    1566 
   Size:        653     600      84     229

  Fragments arranged by size: 

                 653     600     229      84
 
BsmI GAATG_Cn'

Cuts at:      0    1561    1566 
   Size:       1561       5
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     530     653     818     890     972    1106    1157 
   Size:        118     412     123     165      72      82     134      51

Cuts at:   1157    1253    1294    1337    1500    1566 
   Size:         96      41      43     163      66

  Fragments arranged by size: 

                 412     165     163     134     123     118      96      82
                  72      66      51      43      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     422     851    1127    1540    1566 
   Size:        422     429     276     413      26

  Fragments arranged by size: 

                 429     422     413     276      26
 
BsrFI r'CCGG_y

Cuts at:      0     128     244     616    1566 
   Size:        128     116     372     950

  Fragments arranged by size: 

                 950     372     128     116
 
BssHII G'CGCG_C

Cuts at:      0     240     601    1566 
   Size:        240     361     965

  Fragments arranged by size: 

                 965     361     240
 
BssSI C'ACGA_G

Cuts at:      0     978    1524    1566 
   Size:        978     546      42

  Fragments arranged by size: 

                 978     546      42
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     852    1566 
   Size:        852     714
 
BstYI r'GATC_y

Cuts at:      0     893    1271    1566 
   Size:        893     378     295

  Fragments arranged by size: 

                 893     378     295
 
Bsu36I CC'TnA_GG

Cuts at:      0    1369    1566 
   Size:       1369     197
 
BtgI C'CryG_G

Cuts at:      0     471    1229    1295    1566 
   Size:        471     758      66     271

  Fragments arranged by size: 

                 758     471     271      66
 
BtsI GCAGTG_nn'

Cuts at:      0    1027    1149    1566 
   Size:       1027     122     417

  Fragments arranged by size: 

                1027     417     122
 
DraIII CAC_nnn'GTG

Cuts at:      0    1281    1566 
   Size:       1281     285
 
EaeI y'GGCC_r

Cuts at:      0     246     734     932    1113    1127    1169    1566 
   Size:        246     488     198     181      14      42     397

  Fragments arranged by size: 

                 488     397     246     198     181      42      14
 
EagI C'GGCC_G

Cuts at:      0    1127    1566 
   Size:       1127     439
 
EarI CTCTTCn'nnn_

Cuts at:      0     472    1241    1566 
   Size:        472     769     325

  Fragments arranged by size: 

                 769     472     325
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793    1566 
   Size:        793     773
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     172    1087    1566 
   Size:        172     915     479

  Fragments arranged by size: 

                 915     479     172
 
EcoO109I rG'GnC_Cy

Cuts at:      0      47      77    1153    1154    1566 
   Size:         47      30    1076       1     412

  Fragments arranged by size: 

                1076     412      47      30       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     690    1566 
   Size:        690     876
 
FspI TGC'GCA

Cuts at:      0     418     535    1566 
   Size:        418     117    1031

  Fragments arranged by size: 

                1031     418     117
 
FspAI rTGC'GCAy

Cuts at:      0     535    1566 
   Size:        535    1031
 
HaeII r_GCGC'y

Cuts at:      0     163     181     454    1005    1566 
   Size:        163      18     273     551     561

  Fragments arranged by size: 

                 561     551     273     163      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1416    1448    1566 
   Size:       1416      32     118

  Fragments arranged by size: 

                1416     118      32
 
KasI G'GCGC_C

Cuts at:      0     177    1566 
   Size:        177    1389
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     890    1566 
   Size:        389     501     676

  Fragments arranged by size: 

                 676     501     389
 
MscI TGG'CCA

Cuts at:      0     736    1566 
   Size:        736     830
 
MspA1I CmG'CkG

Cuts at:      0     110     123     858    1112    1566 
   Size:        110      13     735     254     454

  Fragments arranged by size: 

                 735     454     254     110      13
 
NaeI GCC'GGC

Cuts at:      0     246     618    1566 
   Size:        246     372     948

  Fragments arranged by size: 

                 948     372     246
 
NarI GG'CG_CC

Cuts at:      0     178    1566 
   Size:        178    1388
 
NcoI C'CATG_G

Cuts at:      0    1295    1566 
   Size:       1295     271
 
NdeI CA'TA_TG

Cuts at:      0     500    1566 
   Size:        500    1066
 
NgoMIV G'CCGG_C

Cuts at:      0     244     616    1566 
   Size:        244     372     950

  Fragments arranged by size: 

                 950     372     244
 
NsiI A_TGCA'T

Cuts at:      0    1433    1566 
   Size:       1433     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1281    1566 
   Size:       1281     285
 
PfoI T'CCnGG_A

Cuts at:      0     542    1058    1345    1566 
   Size:        542     516     287     221

  Fragments arranged by size: 

                 542     516     287     221
 
PmlI CAC'GTG

Cuts at:      0     275    1566 
   Size:        275    1291
 
PspOMI G'GGCC_C

Cuts at:      0    1153    1566 
   Size:       1153     413
 
PspXI vC'TCGA_Gb

Cuts at:      0     646    1566 
   Size:        646     920
 
PstI C_TGCA'G

Cuts at:      0     449     848     857    1566 
   Size:        449     399       9     709

  Fragments arranged by size: 

                 709     449     399       9
 
PvuII CAG'CTG

Cuts at:      0     123     858    1566 
   Size:        123     735     708

  Fragments arranged by size: 

                 735     708     123
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1241    1566 
   Size:       1241     325
 
SfcI C'TryA_G

Cuts at:      0     445     844     853    1566 
   Size:        445     399       9     713

  Fragments arranged by size: 

                 713     445     399       9
 
SfoI GGC'GCC

Cuts at:      0     179    1566 
   Size:        179    1387
 
SmlI C'TyrA_G

Cuts at:      0     559     589     646    1566 
   Size:        559      30      57     920

  Fragments arranged by size: 

                 920     559      57      30
 
StyI C'CwwG_G

Cuts at:      0    1295    1566 
   Size:       1295     271
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       7     391    1566 
   Size:          7     384    1175

  Fragments arranged by size: 

                1175     384       7
 
TatI w'GTAC_w

Cuts at:      0     149     806    1566 
   Size:        149     657     760

  Fragments arranged by size: 

                 760     657     149
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     175     207     289     321     832     864    1487    1519 
   Size:        175      32      82      32     511      32     623      32

Cuts at:   1519    1566 
   Size:         47

  Fragments arranged by size: 

                 623     511     175      82      47      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     661    1566 
   Size:        661     905
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1434    1566 
   Size:       1434     132
 
XhoI C'TCGA_G

Cuts at:      0     646    1566 
   Size:        646     920
 
XmnI GAAnn'nnTTC

Cuts at:      0    1354    1566 
   Size:       1354     212
 
ZraI GAC'GTC

Cuts at:      0    1056    1566 
   Size:       1056     510
 

 Enzymes that do cut:

     AarI    AatII   AflIII    AlwNI     ApaI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI     EarI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I     KasI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NsiI    PflMI     PfoI     PmlI   PspOMI    PspXI     PstI    PvuII
     SapI     SfcI     SfoI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbsI    BglII     BlpI    BmgBI     BmtI    BsaWI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
     EciI  EcoICRI    EcoRI    EcoRV     FseI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MslI     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XmaI
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