Restriction map for
pF1KB6964
(Circular) (Six-base) MAPSORT of: pF1KB6964.seq Check: 3510 from: 1 to: 3836
pF1KB6964 3836 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 469 554 874 469
Size: 85 320 3431
Fragments arranged by size:
3431 320 85
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2840 3108 1661
Size: 432 747 268 2389
Fragments arranged by size:
2389 747 432 268
AflII C'TTAA_G
Cuts at: 819 819
Size: 3836
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 633 1141 2708 633
Size: 508 1567 1761
Fragments arranged by size:
1761 1567 508
ApaI G_GGCC'C
Cuts at: 181 181
Size: 3836
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 840 3189 3445 840
Size: 2349 256 1231
Fragments arranged by size:
2349 1231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 852 1266 1514 1549 852
Size: 414 248 35 3139
Fragments arranged by size:
3139 414 248 35
BanII G_rGCy'C
Cuts at: 181 850 1880 181
Size: 669 1030 2137
Fragments arranged by size:
2137 1030 669
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BbsI GAAGACnn'nnnn_
Cuts at: 717 717
Size: 3836
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 609 948 609
Size: 339 3497
Bme1580I G_kGCm'C
Cuts at: 181 1461 1554 2610 181
Size: 1280 93 1056 1407
Fragments arranged by size:
1407 1280 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 767 1048 1129 1464 3608 767
Size: 281 81 335 2144 995
Fragments arranged by size:
2144 995 335 281 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 623 260
Size: 363 3473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 770 1025 1546 2224 2498 2645 3056 770
Size: 255 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 255 147
BseYI C'CCAG_C
Cuts at: 1261 2019 2596 3092 1261
Size: 758 577 496 2005
Fragments arranged by size:
2005 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 850 1628 1818 2610 850
Size: 778 190 792 2076
Fragments arranged by size:
2076 792 778 190
BsmI GAATG_Cn'
Cuts at: 420 420
Size: 3836
Bsp1286I G_dGCh'C
Cuts at: 181 850 1461 1554 1628 1818 1880 2610 181
Size: 669 611 93 74 190 62 730 1407
Fragments arranged by size:
1407 730 669 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 770 3056 770
Size: 2286 1550
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1100 1402 1783 886
Size: 214 302 381 2939
Fragments arranged by size:
2939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 467 1748 467
Size: 1281 2555
BsrFI r'CCGG_y
Cuts at: 1834 2015 2224 1834
Size: 181 209 3446
Fragments arranged by size:
3446 209 181
BsrGI T'GTAC_A
Cuts at: 752 752
Size: 3836
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 259 457 1213 259
Size: 198 756 2882
Fragments arranged by size:
2882 756 198
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstXI CCAn_nnnn'nTGG
Cuts at: 711 711
Size: 3836
BstYI r'GATC_y
Cuts at: 1 773 861 1328 1351 1686 1932 2944 3030
Size: 772 88 467 23 335 246 1012 86
Cuts at: 3030 3042 3388 1
Size: 12 346 449
Fragments arranged by size:
1012 772 467 449 346 335 246 88
86 23 12
BstZ17I GTA'TAC
Cuts at: 555 555
Size: 3836
BtgI C'CryG_G
Cuts at: 173 173
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 340 1542 2400 340
Size: 1202 858 1776
Fragments arranged by size:
1776 1202 858
EaeI y'GGCC_r
Cuts at: 1421 1595 1986 2013 3358 1421
Size: 174 391 27 1345 1899
Fragments arranged by size:
1899 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 623 1661 2093 2840 3108 260
Size: 363 1038 432 747 268 988
Fragments arranged by size:
1038 988 747 432 363 268
EcoO109I rG'GnC_Cy
Cuts at: 178 975 178
Size: 797 3039
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 492 1270 1518 2540 3519 492
Size: 778 248 1022 979 809
Fragments arranged by size:
1022 979 809 778 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1678 1708 1710 1740 1678
Size: 30 2 30 3774
Fragments arranged by size:
3774 30 30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MfeI C'AATT_G
Cuts at: 233 254 452 233
Size: 21 198 3617
Fragments arranged by size:
3617 198 21
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 1825 1952 1825
Size: 127 3709
MspA1I CmG'CkG
Cuts at: 175 189 356 687 947 1261 1621 2634 2879
Size: 14 167 331 260 314 360 1013 245
Cuts at: 2879 175
Size: 1132
Fragments arranged by size:
1132 1013 360 331 314 260 245 167
14
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NspI r_CATG'y
Cuts at: 666 682 889 1389 2296 666
Size: 16 207 500 907 2206
Fragments arranged by size:
2206 907 500 207 16
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PspOMI G'GGCC_C
Cuts at: 177 177
Size: 3836
PstI C_TGCA'G
Cuts at: 883 1568 883
Size: 685 3151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 189 1261 1621 189
Size: 1072 360 2404
Fragments arranged by size:
2404 1072 360
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SacII CC_GC'GG
Cuts at: 176 176
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SexAI A'CCwGG_T
Cuts at: 644 644
Size: 3836
SfcI C'TryA_G
Cuts at: 33 119 625 879 1564 2557 2748 33
Size: 86 506 254 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 254 191 86
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 819 991 1906 2398 2660 819
Size: 172 915 492 262 1995
Fragments arranged by size:
1995 915 492 262 172
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 692 970 1324 692
Size: 278 354 3204
Fragments arranged by size:
3204 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 1552 2011 2220 1419
Size: 133 459 209 3035
Fragments arranged by size:
3035 459 209 133
TatI w'GTAC_w
Cuts at: 752 3351 752
Size: 2599 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 3804
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI