Restriction map for pF1KB9870
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      Eam1105I    Eco47III    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp509I     XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AfaI           30  GTAC                 GT^AC
AlwI          765  GATCC                GGATC(4/5)
ApaI          109  GGGCCC               GGGCC^C
Asp700I      1419  GAATGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           33  CCCGGG               C^[CT]CG[GA]G
AviII         184  TGCGCA               TGC^GCA
BalI          590  TGGCCA               TGG^CCA
BcgI          630  CGACGTGAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          899  TGATCA               T^GATCA
BglII        1316  AGATCT               A^GATCT
BsaWI         522  TCCGGT               [AT]^CCGG[AT]
BsmBI        1153  GAGACG               CGTCTC(1/5)
BsmI          636  GAATGC               GAATGC(1/-1)
BsoBI          33  CCCGGG               C^[CT]CG[GA]G
BspHI         719  TCATGA               T^CATGA
BsrDI        1169  GCAATG               GCAATG(2/0)
DrdI         1024  GACGTCTTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           77  CCGTGG               C^C[GA][CT]GG
EagI          989  CGGCCG               C^GGCCG
EarI          681  CTCTTC               CTCTTC(1/4)
EclXI         989  CGGCCG               C^GGCCG
Eco52I        989  CGGCCG               C^GGCCG
FbaI          899  TGATCA               T^GATCA
FspI          184  TGCGCA               TGC^GCA
HaeII         270  GGCGCT               [GA]GCGC^[CT]
Ksp632I       681  CTCTTC               CTCTTC(1/4)
MluNI         590  TGGCCA               TGG^CCA
MscI          590  TGGCCA               TGG^CCA
MseI          944  TTAA                 T^TAA
NaeI          991  GCCGGC               GCC^GGC
NgoMIV        991  GCCGGC               G^CCGGC
NspI         1307  GCATGT               [GA]CATG^[CT]
PpuMI        1293  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         225  GACCAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          963  CTGCAG               CTGCA^G
PvuI         1302  CGATCG               CGAT^CG
RcaI          719  TCATGA               T^CATGA
RsaI           30  GTAC                 GT^AC
SacI         1174  GAGCTC               GAGCT^C
SfcI          963  CTGCAG               C^T[GA][CT]AG
SgrAI        1145  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI           33  CCCGGG               CCC^GGG
SmlI          883  CTCAAG               C^T[CT][GA]AG
Tru9I         944  TTAA                 T^TAA
XmaCI          33  CCCGGG               C^CCGGG
XmaI           33  CCCGGG               C^CCGGG
XmnI         1419  GAATGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI          55  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1111  CAGCTGCTG           
BanI           22  GGCACC               G^G[CT][GA]CC
               96  GGCACC              
BglI          102  GCCACTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              301  GCCGAGGTGGC         
BsaI          871  GAGACC               GGTCTC(1/5)
             1012  GAGACC              
BsgI          760  GTGCAG               GTGCAG(16/14)
             1122  GTGCAG              
BsiEI         989  CGGCCG               CG[GA][CT]^CG
             1302  CGATCG              
BspMI         209  ACCTGC               ACCTGC(4/8)
              575  ACCTGC              
BstEII        123  GGTCACC              G^GT[ACGT]ACC
             1339  GGTGACC             
BstPI         123  GGTCACC              G^GT[ACGT]ACC
             1339  GGTGACC             
BstYI         764  AGATCC               [GA]^GATC[CT]
             1316  AGATCT              
DdeI          458  CTCAG                C^T[ACGT]AG
             1352  CTGAG               
EcoO65I       123  GGTCACC              G^GT[ACGT]ACC
             1339  GGTGACC             
EcoT14I       470  CCTTGG               C^C[AT][AT]GG
              875  CCAAGG              
MflI          764  AGATCC               [GA]^GATC[CT]
             1316  AGATCT              
PflMI          54  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1110  CCAGCTGCTGG         
StyI          470  CCTTGG               C^C[AT][AT]GG
              875  CCAAGG              
TfiI          663  GAATC                G^A[AT]TC
             1424  GATTC               
Van91I         54  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1110  CCAGCTGCTGG         
XhoII         764  AGATCC               [GA]^GATC[CT]
             1316  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AluI        
AspHI       AspI        AvaII       BanII       BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrFI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        Tsp45I      TspRI       Tth111I     
TthHB8I     
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