Restriction map for pF1KB6949
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AvaII       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BcnI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmFI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
MaeII       MamI        MfeI        MflI        MluI        
MroI        MspI        MunI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TfiI        Van91I      XbaI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        581  GGTACC               G^GTACC
AlwI          456  GATCC                GGATC(4/5)
Asp718I       581  GGTACC               G^GTACC
AspI          157  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          239  CTCGAG               C^[CT]CG[GA]G
BanI          581  GGTACC               G^G[CT][GA]CC
BbvI          188  GCTGC                GCAGC(8/12)
BcgI          231  GCAGGCCACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          520  CTAG                 C^TAG
BglI          226  GCCTTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          255  GGTCTC               GGTCTC(1/5)
BsgI           69  CTGCAC               GTGCAG(16/14)
BsmBI         326  GAGACG               CGTCTC(1/5)
BsoBI         239  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      137  TGTACA               T^GTACA
BspMI         385  GCAGGT               ACCTGC(4/8)
BsrBI         594  CCGCTC               CCGCTC(-3/-3)
BsrGI         137  TGTACA               T^GTACA
BstXI         281  CCATGTTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          315  GCGC                 GCG^C
DdeI          605  CTAAG                C^T[ACGT]AG
DpnII         456  GATC                 ^GATC
EarI          615  CTCTTC               CTCTTC(1/4)
HgaI          161  GCGTC                GACGC(5/10)
HhaI          315  GCGC                 GCG^C
HinP1I        315  GCGC                 G^CGC
HinfI         202  GACTC                G^A[ACGT]TC
HphI          109  GGTGA                GGTGA(8/7)
KpnI          581  GGTACC               GGTAC^C
Ksp632I       615  CTCTTC               CTCTTC(1/4)
KspI          221  CCGCGG               CCGC^GG
MaeI          520  CTAG                 C^TAG
MboI          456  GATC                 ^GATC
MseI          470  TTAA                 T^TAA
MspA1I        221  CCGCGG               C[AC]G^C[GT]G
NdeII         456  GATC                 ^GATC
NlaIV         581  GGTACC               GG[ACGT]^[ACGT]CC
PaeR7I        239  CTCGAG               C^TCGAG
PflFI         157  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          202  GACTC                GAGTC(4/5)
PstI           16  CTGCAG               CTGCA^G
SacI           96  GAGCTC               GAGCT^C
SacII         221  CCGCGG               CCGC^GG
Sau3AI        456  GATC                 ^GATC
SfcI           16  CTGCAG               C^T[GA][CT]AG
SfiI          225  GGCCTTGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          239  CTCGAG               C^T[CT][GA]AG
SspBI         137  TGTACA               T^GTACA
TaqI          240  TCGA                 T^CGA
Tru9I         470  TTAA                 T^TAA
TseI          188  GCTGC                G^C[AT]GC
Tth111I       157  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       240  TCGA                 T^CGA
XcmI           35  CCATGGCCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          239  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccII         222  CGCG                 CG^CG
              314  CGCG                
AlwNI          19  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              509  CAGGGTCTG           
BanII          96  GAGCTC               G[GA]GC[CT]^C
              242  GAGCCC              
BsmAI         326  GAGAC                GTCTC(1/5)
              256  GTCTC               
BstUI         222  CGCG                 CG^CG
              314  CGCG                
MboII         339  GAAGA                GAAGA(8/7)
              616  TCTTC               
MnlI          365  GAGG                 CCTC(7/6)
              533  GAGG                
MslI          174  CACGGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              282  CATGTTTGTG          
MvnI          222  CGCG                 CG^CG
              314  CGCG                
NcoI           35  CCATGG               C^CATGG
              356  CCATGG              
Tsp509I         7  AATT                 ^AATT
              372  AATT                
TspRI          85  CAGTG                CA[GC]TG[ACGT][ACGT]^
              539  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      ApaLI       
AspHI       BalI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BssKI       BstAPI      
BstF5I      BstNI       Cac8I       Cfr13I      DsaI        
EaeI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      ItaI        MaeIII      MluNI       
MscI        MvaI        MwoI        NlaIII      RsaI        
Sau96I      ScrFI       StyI        Tsp45I      
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