Restriction map for pF1KB6944
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
AlwI        AlwNI       AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraII       DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        TseI        Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AflIII        566  ACATGT               A^C[GA][CT]GT
Alw44I        535  GTGCAC               G^TGCAC
ApaI          503  GGGCCC               GGGCC^C
ApaLI         535  GTGCAC               G^TGCAC
AvaII         144  GGTCC                G^G[AT]CC
BalI          492  TGGCCA               TGG^CCA
BbsI          124  GTCTTC               GAAGAC(2/6)
BfaI          514  CTAG                 C^TAG
BglI          590  GCCGCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         167  AGATCT               A^GATCT
BpuAI         124  GTCTTC               GAAGAC(2/6)
BspHI         561  TCATGA               T^CATGA
BspLU11I      566  ACATGT               A^CATGT
BsrFI          36  GCCGGT               [GA]^CCGG[CT]
BstAPI        624  GCACTCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         281  CCAAGATTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         167  AGATCT               [GA]^GATC[CT]
Cfr10I         36  GCCGGT               [GA]^CCGG[CT]
DpnII         168  GATC                 ^GATC
DrdI          136  GACTCCTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          315  CCACGG               C^C[GA][CT]GG
EaeI          492  TGGCCA               [CT]^GGCC[GA]
EarI          181  CTCTTC               CTCTTC(1/4)
FauI          685  CCCGC                CCCGC(4/6)
HaeII          62  GGCGCT               [GA]GCGC^[CT]
HgaI          639  GCGTC                GACGC(5/10)
HinfI         136  GACTC                G^A[ACGT]TC
Ksp632I       181  CTCTTC               CTCTTC(1/4)
MaeI          514  CTAG                 C^TAG
MaeII          47  ACGT                 A^CGT
MboI          168  GATC                 ^GATC
MflI          167  AGATCT               [GA]^GATC[CT]
MluNI         492  TGGCCA               TGG^CCA
MscI          492  TGGCCA               TGG^CCA
MseI          203  TTAA                 T^TAA
NdeII         168  GATC                 ^GATC
PleI          136  GACTC                GAGTC(4/5)
RcaI          561  TCATGA               T^CATGA
SacI          178  GAGCTC               GAGCT^C
SapI          180  GCTCTTC              GCTCTTC(1/4)
Sau3AI        168  GATC                 ^GATC
SgrAI          35  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI          392  CCCGGG               CCC^GGG
SphI          195  GCATGC               GCATG^C
Tru9I         203  TTAA                 T^TAA
XhoII         167  AGATCT               [GA]^GATC[CT]
XmaCI         392  CCCGGG               C^CCGGG
XmaI          392  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII         175  CGCG                 CG^CG
              419  CGCG                
AvaI          392  CCCGGG               C^[CT]CG[GA]G
              500  CTCGGG              
BanI          304  GGCACC               G^G[CT][GA]CC
              547  GGCACC              
BpmI          250  CTGGAG               CTGGAG(16/14)
              679  CTGGAG              
BseRI         188  CTCCTC               GAGGAG(10/8)
               10  GAGGAG              
BsmFI         134  GGGAC                GGGAC(10/14)
               72  GTCCC               
BsoBI         392  CCCGGG               C^[CT]CG[GA]G
              500  CTCGGG              
BspMI         578  ACCTGC               ACCTGC(4/8)
              333  GCAGGT              
BstUI         175  CGCG                 CG^CG
              419  CGCG                
CfoI           63  GCGC                 GCG^C
              174  GCGC                
DdeI          540  CTCAG                C^T[ACGT]AG
              586  CTGAG               
EcoT14I       140  CCTTGG               C^C[AT][AT]GG
              663  CCAAGG              
HhaI           63  GCGC                 GCG^C
              174  GCGC                
HinP1I         63  GCGC                 G^CGC
              174  GCGC                
MaeIII        221  GTGAC                ^GT[ACGT]AC
              337  GTGAC               
MslI           33  CACGCCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              538  CACTCAGATG          
MvnI          175  CGCG                 CG^CG
              419  CGCG                
StyI          140  CCTTGG               C^C[AT][AT]GG
              663  CCAAGG              
Tsp45I        221  GTGAC                ^GT[GC]AC
              337  GTGAC               
TspRI         160  CAGTG                CA[GC]TG[ACGT][ACGT]^
              485  CACTG               
XcmI          281  CCAAGATTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              356  CCATTGTGCAACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BanII       
BcnI        BmyI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
NspI        RsaI        Sau96I      ScrFI       SfaNI       
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