Restriction map for pF1KB6944
 (Linear) (Six-base) MAPSORT of: KIBB6944.seq  Check: 4233  from: 1  to: 693



KIBB6944.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     324     693 
   Size:        324     369
 
AflIII A'CryG_T

Cuts at:      0     566     693 
   Size:        566     127
 
AleI CACnn'nnGTG

Cuts at:      0      37     693 
   Size:         37     656
 
ApaI G_GGCC'C

Cuts at:      0     507     693 
   Size:        507     186
 
ApaLI G'TGCA_C

Cuts at:      0     535     693 
   Size:        535     158
 
AvaI C'yCGr_G

Cuts at:      0     392     500     693 
   Size:        392     108     193

  Fragments arranged by size: 

                 392     193     108
 
BanI G'GyrC_C

Cuts at:      0     304     547     693 
   Size:        304     243     146

  Fragments arranged by size: 

                 304     243     146
 
BanII G_rGCy'C

Cuts at:      0     182     507     621     686     693 
   Size:        182     325     114      65       7

  Fragments arranged by size: 

                 325     182     114      65       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     117     693 
   Size:        117     576
 
BglI GCCn_nnn'nGGC

Cuts at:      0     596     693 
   Size:        596      97
 
BglII A'GATC_T

Cuts at:      0     167     693 
   Size:        167     526
 
Bme1580I G_kGCm'C

Cuts at:      0     307     507     539     693 
   Size:        307     200      32     154

  Fragments arranged by size: 

                 307     200     154      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     201     693 
   Size:        201     492
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     693 
   Size:        271     422
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     179     693 
   Size:         25     154     514

  Fragments arranged by size: 

                 514     154      25
 
BseYI C'CCAG_C

Cuts at:      0      18     405     693 
   Size:         18     387     288

  Fragments arranged by size: 

                 387     288      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     607     693 
   Size:         61     546      86

  Fragments arranged by size: 

                 546      86      61
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     539     672     693 
   Size:        182     357     133      21

  Fragments arranged by size: 

                 357     182     133      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     307     507     539     621     672     686     693 
   Size:        182     125     200      32      82      51      14       7

  Fragments arranged by size: 

                 200     182     125      82      51      32      14       7
            
 
BspHI T'CATG_A

Cuts at:      0     561     693 
   Size:        561     132
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     324     587     693 
   Size:        324     263     106

  Fragments arranged by size: 

                 324     263     106
 
BsrFI r'CCGG_y

Cuts at:      0      36     693 
   Size:         36     657
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     630     693 
   Size:        630      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     693 
   Size:        288     405
 
BstYI r'GATC_y

Cuts at:      0     167     693 
   Size:        167     526
 
BtgI C'CryG_G

Cuts at:      0     315     693 
   Size:        315     378
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     142     693 
   Size:        142     551
 
EaeI y'GGCC_r

Cuts at:      0     492     693 
   Size:        492     201
 
EarI CTCTTCn'nnn_

Cuts at:      0     187     693 
   Size:        187     506
 
EcoICRI GAG'CTC

Cuts at:      0     180     693 
   Size:        180     513
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     693 
   Size:        271     422
 
HaeII r_GCGC'y

Cuts at:      0      66     693 
   Size:         66     627
 
MscI TGG'CCA

Cuts at:      0     494     693 
   Size:        494     199
 
MslI CAynn'nnrTG

Cuts at:      0      37     542     693 
   Size:         37     505     151

  Fragments arranged by size: 

                 505     151      37
 
NspI r_CATG'y

Cuts at:      0     199     238     570     693 
   Size:        199      39     332     123

  Fragments arranged by size: 

                 332     199     123      39
 
PciI A'CATG_T

Cuts at:      0     566     693 
   Size:        566     127
 
PspOMI G'GGCC_C

Cuts at:      0     503     693 
   Size:        503     190
 
SacI G_AGCT'C

Cuts at:      0     182     693 
   Size:        182     511
 
SapI GCTCTTCn'nnn_

Cuts at:      0     187     693 
   Size:        187     506
 
SgrAI Cr'CCGG_yG

Cuts at:      0      36     693 
   Size:         36     657
 
SmaI CCC'GGG

Cuts at:      0     394     693 
   Size:        394     299
 
SphI G_CATG'C

Cuts at:      0     199     693 
   Size:        199     494
 
StyI C'CwwG_G

Cuts at:      0     140     663     693 
   Size:        140     523      30

  Fragments arranged by size: 

                 523     140      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     288     693 
   Size:        288     405
 
TatI w'GTAC_w

Cuts at:      0     237     693 
   Size:        237     456
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     288     363     693 
   Size:        288      75     330

  Fragments arranged by size: 

                 330     288      75
 
XmaI C'CCGG_G

Cuts at:      0     392     693 
   Size:        392     301
 

 Enzymes that do cut:

     AarI   AflIII     AleI     ApaI    ApaLI     AvaI     BanI    BanII
     BbsI     BglI    BglII Bme1580I     BplI     BpmI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrFI   BstAPI    BstXI
    BstYI     BtgI     DrdI     EaeI     EarI  EcoICRI  Eco57MI    HaeII
     MscI     MslI     NspI     PciI   PspOMI     SacI     SapI    SgrAI
     SmaI     SphI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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