Restriction map for pF1KB6943
 (Linear) (Six-base) MAPSORT of: KIBB6943.seq  Check: 9196  from: 1  to: 549



KIBB6943.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AfeI AGC'GCT

Cuts at:      0      26     549 
   Size:         26     523
 
AflIII A'CryG_T

Cuts at:      0     299     549 
   Size:        299     250
 
AvaI C'yCGr_G

Cuts at:      0     158     213     549 
   Size:        158      55     336

  Fragments arranged by size: 

                 336     158      55
 
BanI G'GyrC_C

Cuts at:      0     165     549 
   Size:        165     384
 
BanII G_rGCy'C

Cuts at:      0     475     549 
   Size:        475      74
 
BbeI G_GCGC'C

Cuts at:      0     169     549 
   Size:        169     380
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     499     531     549 
   Size:        499      32      18

  Fragments arranged by size: 

                 499      32      18
 
BglI GCCn_nnn'nGGC

Cuts at:      0     163     549 
   Size:        163     386
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     435     549 
   Size:        435     114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     145     374     380     549 
   Size:        106      39     229       6     169

  Fragments arranged by size: 

                 229     169     106      39       6
 
BsaHI Gr'CG_yC

Cuts at:      0     166     549 
   Size:        166     383
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114     549 
   Size:        114     435
 
BseYI C'CCAG_C

Cuts at:      0     401     549 
   Size:        401     148
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     549 
   Size:        280     269
 
BsiEI CG_ry'CG

Cuts at:      0      20     549 
   Size:         20     529
 
Bsp1286I G_dGCh'C

Cuts at:      0     475     549 
   Size:        475      74
 
BsrFI r'CCGG_y

Cuts at:      0     186     193     549 
   Size:        186       7     356

  Fragments arranged by size: 

                 356     186       7
 
EaeI y'GGCC_r

Cuts at:      0      17     549 
   Size:         17     532
 
EagI C'GGCC_G

Cuts at:      0      17     549 
   Size:         17     532
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     145     374     380     549 
   Size:        106      39     229       6     169

  Fragments arranged by size: 

                 229     169     106      39       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     426     452     549 
   Size:        150     276      26      97

  Fragments arranged by size: 

                 276     150      97      26
 
HaeII r_GCGC'y

Cuts at:      0      28     169     549 
   Size:         28     141     380

  Fragments arranged by size: 

                 380     141      28
 
KasI G'GCGC_C

Cuts at:      0     165     549 
   Size:        165     384
 
NaeI GCC'GGC

Cuts at:      0     188     195     549 
   Size:        188       7     354

  Fragments arranged by size: 

                 354     188       7
 
NarI GG'CG_CC

Cuts at:      0     166     549 
   Size:        166     383
 
NgoMIV G'CCGG_C

Cuts at:      0     186     193     549 
   Size:        186       7     356

  Fragments arranged by size: 

                 356     186       7
 
NotI GC'GGCC_GC

Cuts at:      0      17     549 
   Size:         17     532
 
NspI r_CATG'y

Cuts at:      0     121     303     388     549 
   Size:        121     182      85     161

  Fragments arranged by size: 

                 182     161     121      85
 
PciI A'CATG_T

Cuts at:      0     299     549 
   Size:        299     250
 
PfoI T'CCnGG_A

Cuts at:      0     357     549 
   Size:        357     192
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     426     452     549 
   Size:        150     276      26      97

  Fragments arranged by size: 

                 276     150      97      26
 
PstI C_TGCA'G

Cuts at:      0      56     549 
   Size:         56     493
 
SexAI A'CCwGG_T

Cuts at:      0     533     549 
   Size:        533      16
 
SfcI C'TryA_G

Cuts at:      0      52     549 
   Size:         52     497
 
SfoI GGC'GCC

Cuts at:      0     167     549 
   Size:        167     382
 
SmaI CCC'GGG

Cuts at:      0     160     215     549 
   Size:        160      55     334

  Fragments arranged by size: 

                 334     160      55
 
SphI G_CATG'C

Cuts at:      0     121     388     549 
   Size:        121     267     161

  Fragments arranged by size: 

                 267     161     121
 
SspI AAT'ATT

Cuts at:      0     226     549 
   Size:        226     323
 
StyI C'CwwG_G

Cuts at:      0     351     549 
   Size:        351     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      56     549 
   Size:         56     493
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     512     544     549 
   Size:        512      32       5

  Fragments arranged by size: 

                 512      32       5
 
Tth111I GACn'n_nGTC

Cuts at:      0     535     549 
   Size:        535      14
 
XbaI T'CTAG_A

Cuts at:      0      75     549 
   Size:         75     474
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141     549 
   Size:        141     408
 
XmaI C'CCGG_G

Cuts at:      0     158     213     549 
   Size:        158      55     336

  Fragments arranged by size: 

                 336     158      55
 

 Enzymes that do cut:

     AfeI   AflIII     AvaI     BanI    BanII     BbeI     BbsI     BglI
     BplI     BpmI    BsaHI    BseRI    BseYI     BsgI    BsiEI Bsp1286I
    BsrFI     EaeI     EagI  Eco57MI EcoO109I    HaeII     KasI     NaeI
     NarI   NgoMIV     NotI     NspI     PciI     PfoI    PpuMI     PstI
    SexAI     SfcI     SfoI     SmaI     SphI     SspI     StyI    TaqII
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     StuI     SwaI     TatI     XhoI     XmnI     ZraI
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