Restriction map for
pF1KB6938
(Circular) (Six-base) MAPSORT of: pF1KB6938.seq Check: 3885 from: 1 to: 3800
pF1KB6938 3800 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 838 838
Size: 3800
Acc65I G'GTAC_C
Cuts at: 816 816
Size: 3800
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 541 1625 2057 2804 3072 441
Size: 100 1084 432 747 268 1169
Fragments arranged by size:
1169 1084 747 432 268 100
AfeI AGC'GCT
Cuts at: 343 343
Size: 3800
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 410 1105 2672 410
Size: 695 1567 1538
Fragments arranged by size:
1567 1538 695
ApaLI G'TGCA_C
Cuts at: 2570 2570
Size: 3800
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 820 820
Size: 3800
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 816 1230 1478 1513 816
Size: 414 248 35 3103
Fragments arranged by size:
3103 414 248 35
BanII G_rGCy'C
Cuts at: 814 1844 814
Size: 1030 2770
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3766
BciVI GTATCCnnnnn_n'
Cuts at: 118 1698 2459 3800 118
Size: 1580 761 1341 118
Fragments arranged by size:
1580 1341 761 118
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BglII A'GATC_T
Cuts at: 1315 3352 1315
Size: 2037 1763
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 385 1425 1518 2574 385
Size: 1040 93 1056 1611
Fragments arranged by size:
1611 1056 1040 93
BmrI ACTGGGnnnn_n'
Cuts at: 1012 1093 1428 3572 1012
Size: 81 335 2144 1240
Fragments arranged by size:
2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 648 2167 648
Size: 1519 2281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 444
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1855 2347 2645 976
Size: 879 492 298 2131
Fragments arranged by size:
2131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3504 3504
Size: 3800
BsaAI yAC'GTr
Cuts at: 1153 1153
Size: 3800
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 1231 1479 2181 3677 1231
Size: 248 702 1496 1354
Fragments arranged by size:
1496 1354 702 248
BsaWI w'CCGG_w
Cuts at: 533 989 1510 2188 2462 2609 3020 533
Size: 456 521 678 274 147 411 1313
Fragments arranged by size:
1313 678 521 456 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 177
Size: 3800
BseYI C'CCAG_C
Cuts at: 1225 1983 2560 3056 1225
Size: 758 577 496 1969
Fragments arranged by size:
1969 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 480
Size: 3800
BsiEI CG_ry'CG
Cuts at: 104 1342 1388 2596 104
Size: 1238 46 1208 1308
Fragments arranged by size:
1308 1238 1208 46
BsiHKAI G_wGCw'C
Cuts at: 814 1592 1782 2574 814
Size: 778 190 792 2040
Fragments arranged by size:
2040 792 778 190
BsmI GAATG_Cn'
Cuts at: 513 513
Size: 3800
BsmBI CGTCTCn'nnnn_
Cuts at: 171 171
Size: 3800
Bsp1286I G_dGCh'C
Cuts at: 385 814 1425 1518 1592 1782 1844 2574 385
Size: 429 611 93 74 190 62 730 1611
Fragments arranged by size:
1611 730 611 429 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 1064 1366 1747 850
Size: 214 302 381 2903
Fragments arranged by size:
2903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2092 2146 3630 3795 50
Size: 2042 54 1484 165 55
Fragments arranged by size:
2042 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1712 1712
Size: 3800
BsrFI r'CCGG_y
Cuts at: 1798 1979 2188 1798
Size: 181 209 3410
Fragments arranged by size:
3410 209 181
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1177
Size: 3800
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstYI r'GATC_y
Cuts at: 1 825 1292 1315 1650 1896 2908 2994 3006
Size: 824 467 23 335 246 1012 86 12
Cuts at: 3006 3352 1
Size: 346 449
Fragments arranged by size:
1012 824 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1750 1931 2094 1750
Size: 181 163 3456
Fragments arranged by size:
3456 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1043
Size: 3800
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1506 2364 1506
Size: 858 2942
EaeI y'GGCC_r
Cuts at: 1385 1559 1950 1977 3322 1385
Size: 174 391 27 1345 1863
Fragments arranged by size:
1863 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 473 1823 2033 473
Size: 1350 210 2240
Fragments arranged by size:
2240 1350 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 444 541 1625 2057 2804 3072 441
Size: 3 97 1084 432 747 268 1169
Fragments arranged by size:
1169 1084 747 432 268 97 3
EcoO109I rG'GnC_Cy
Cuts at: 939 939
Size: 3800
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 345 1234 1482 2504 3483 345
Size: 889 248 1022 979 662
Fragments arranged by size:
1022 979 889 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1642 1672 1674 1704 1642
Size: 30 2 30 3738
Fragments arranged by size:
3738 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 820 820
Size: 3800
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 564 2471 2655 564
Size: 1907 184 1709
Fragments arranged by size:
1907 1709 184
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 502 1789 1916 502
Size: 1287 127 2386
Fragments arranged by size:
2386 1287 127
MspA1I CmG'CkG
Cuts at: 911 1225 1585 2598 2843 911
Size: 314 360 1013 245 1868
Fragments arranged by size:
1868 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NspI r_CATG'y
Cuts at: 853 1353 2260 853
Size: 500 907 2393
Fragments arranged by size:
2393 907 500
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PstI C_TGCA'G
Cuts at: 847 1532 847
Size: 685 3115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 1225 1585 1225
Size: 360 3440
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 493 3317 493
Size: 2824 976
SfcI C'TryA_G
Cuts at: 33 843 1528 2521 2712 33
Size: 810 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 191
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 822 822
Size: 3800
SmlI C'TyrA_G
Cuts at: 955 1870 2362 2624 955
Size: 915 492 262 2131
Fragments arranged by size:
2131 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 853 1353 853
Size: 500 3300
SspI AAT'ATT
Cuts at: 141 141
Size: 3800
StyI C'CwwG_G
Cuts at: 776 934 1288 776
Size: 158 354 3288
Fragments arranged by size:
3288 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1516 1975 2184 1383
Size: 133 459 209 2999
Fragments arranged by size:
2999 459 209 133
TatI w'GTAC_w
Cuts at: 491 548 3315 491
Size: 57 2767 976
Fragments arranged by size:
2767 976 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1295 1295
Size: 3800
XmaI C'CCGG_G
Cuts at: 820 820
Size: 3800
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI