Restriction map for pF1KB6932
 (Linear) (Six-base) MAPSORT of: KIBB6932.seq  Check: 5295  from: 1  to: 684



KIBB6932.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AclI AA'CG_TT

Cuts at:      0     669     684 
   Size:        669      15
 
AlwNI CAG_nnn'CTG

Cuts at:      0     658     684 
   Size:        658      26
 
ApoI r'AATT_y

Cuts at:      0     451     684 
   Size:        451     233
 
AvaI C'yCGr_G

Cuts at:      0     283     684 
   Size:        283     401
 
BanI G'GyrC_C

Cuts at:      0     384     684 
   Size:        384     300
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     617     684 
   Size:        617      67
 
BclI T'GATC_A

Cuts at:      0     626     684 
   Size:        626      58
 
BglII A'GATC_T

Cuts at:      0     619     684 
   Size:        619      65
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     489     684 
   Size:        489     195
 
BsaHI Gr'CG_yC

Cuts at:      0       4     684 
   Size:          4     680
 
BsaWI w'CCGG_w

Cuts at:      0      71     684 
   Size:         71     613
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     618     684 
   Size:        618      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     578     684 
   Size:         85     493     106

  Fragments arranged by size: 

                 493     106      85
 
BspEI T'CCGG_A

Cuts at:      0      71     684 
   Size:         71     613
 
BstBI TT'CG_AA

Cuts at:      0     171     684 
   Size:        171     513
 
BstEII G'GTnAC_C

Cuts at:      0     148     684 
   Size:        148     536
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     233     684 
   Size:        233     451
 
BstYI r'GATC_y

Cuts at:      0     619     684 
   Size:        619      65
 
BtgI C'CryG_G

Cuts at:      0     388     684 
   Size:        388     296
 
BtsI GCAGTG_nn'

Cuts at:      0     597     684 
   Size:        597      87
 
DraIII CAC_nnn'GTG

Cuts at:      0     156     684 
   Size:        156     528
 
EaeI y'GGCC_r

Cuts at:      0      47     684 
   Size:         47     637
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     491     535     684 
   Size:        189     302      44     149

  Fragments arranged by size: 

                 302     189     149      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     604     611     636     643     684 
   Size:        604       7      25       7      41

  Fragments arranged by size: 

                 604      41      25       7       7
 
MscI TGG'CCA

Cuts at:      0      49     684 
   Size:         49     635
 
MslI CAynn'nnrTG

Cuts at:      0     208     214     554     580     684 
   Size:        208       6     340      26     104

  Fragments arranged by size: 

                 340     208     104      26       6
 
NcoI C'CATG_G

Cuts at:      0     388     684 
   Size:        388     296
 
NspI r_CATG'y

Cuts at:      0     207     684 
   Size:        207     477
 
PasI CC'CwG_GG

Cuts at:      0     301     684 
   Size:        301     383
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     491     535     684 
   Size:        189     302      44     149

  Fragments arranged by size: 

                 302     189     149      44
 
SfcI C'TryA_G

Cuts at:      0     251     684 
   Size:        251     433
 
SmlI C'TyrA_G

Cuts at:      0     283     684 
   Size:        283     401
 
StyI C'CwwG_G

Cuts at:      0     388     413     684 
   Size:        388      25     271

  Fragments arranged by size: 

                 388     271      25
 
TatI w'GTAC_w

Cuts at:      0     269     684 
   Size:        269     415
 
Tth111I GACn'n_nGTC

Cuts at:      0     544     684 
   Size:        544     140
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     479     684 
   Size:        479     205
 
XhoI C'TCGA_G

Cuts at:      0     283     684 
   Size:        283     401
 
XmnI GAAnn'nnTTC

Cuts at:      0     167     684 
   Size:        167     517
 

 Enzymes that do cut:

     AclI    AlwNI     ApoI     AvaI     BanI     BbsI     BclI    BglII
     BmrI    BsaHI    BsaWI    BseRI     BsgI    BspEI    BstBI   BstEII
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI EcoO109I    Hin4I
     MscI     MslI     NcoI     NspI     PasI    PpuMI     SfcI     SmlI
     StyI     TatI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI     XbaI     XmaI     ZraI
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