Restriction map for pF1KB6930
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BmyI        BpmI        BpuAI       BsaAI       BsaI        
BsaWI       BseAI       BsiHKAI     BsiWI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AcyI          133  GGCGCC               G[GA]^CG[CT]C
AlwI          324  GGATC                GGATC(4/5)
Asp700I       354  GAATAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         146  GGACC                G^G[AT]CC
BanI          133  GGCGCC               G^G[CT][GA]CC
BbeI          133  GGCGCC               GGCGC^C
BcnI          130  CCCGG                CC^[GC]GG
BlpI            3  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        3  GCTGAGC              GC^T[ACGT]AGC
BsaBI         294  GATAACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         133  GGCGCC               G[GA]^CG[CT]C
BseRI          76  CTCCTC               GAGGAG(10/8)
BsgI          661  GTGCAG               GTGCAG(16/14)
BsiEI          67  CGGCCG               CG[GA][CT]^CG
BsmAI          92  GTCTC                GTCTC(1/5)
BsmBI          91  CGTCTC               CGTCTC(1/5)
BsmFI         145  GGGAC                GGGAC(10/14)
BspHI          95  TCATGA               T^CATGA
BstNI          36  CCTGG                CC^[AT]GG
CelII           3  GCTGAGC              GC^T[ACGT]AGC
DpnII         325  GATC                 ^GATC
DraII         145  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          416  CCATGG               C^C[GA][CT]GG
EaeI           67  CGGCCG               [CT]^GGCC[GA]
EagI           67  CGGCCG               C^GGCCG
EclXI          67  CGGCCG               C^GGCCG
Eco52I         67  CGGCCG               C^GGCCG
EcoO109I      145  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         548  GAATTC               G^AATTC
EcoRII         36  CCTGG                ^CC[AT]GG
EcoT14I       416  CCATGG               C^C[AT][AT]GG
HaeII         133  GGCGCC               [GA]GCGC^[CT]
Hin1I         133  GGCGCC               G[GA]^CG[CT]C
HincII        567  GTCAAC               GT[CT]^[GA]AC
HindII        567  GTCAAC               GT[CT]^[GA]AC
HphI          244  GGTGA                GGTGA(8/7)
KasI          133  GGCGCC               G^GCGCC
MamI          294  GATAACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          325  GATC                 ^GATC
MvaI           36  CCTGG                CC^[AT]GG
NarI          133  GGCGCC               GG^CGCC
NciI          130  CCCGG                CC^[GC]GG
NcoI          416  CCATGG               C^CATGG
NdeII         325  GATC                 ^GATC
PflMI          30  CCACGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         145  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI           95  TCATGA               T^CATGA
Sau3AI        325  GATC                 ^GATC
SfoI          133  GGCGCC               GGC^GCC
SmlI          490  CTTGAG               C^T[CT][GA]AG
StyI          416  CCATGG               C^C[AT][AT]GG
TaqI          167  TCGA                 T^CGA
TspRI         151  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       167  TCGA                 T^CGA
Van91I         30  CCACGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          354  GAATAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          358  AAATTC               [GA]^AATT[CT]
              548  GAATTC              
AfaI          261  GTAC                 GT^AC
              283  GTAC                
ApoI          358  AAATTC               [GA]^AATT[CT]
              548  GAATTC              
BsrI          150  CCAGT                ACTGG(1/-1)
              286  CCAGT               
BssKI          36  CCTGG                ^CC[ACGT]GG
              130  CCCGG               
BstF5I        403  GGATG                GGATG(2/0)
              420  GGATG               
CfoI          134  GCGC                 GCG^C
              155  GCGC                
FauI           62  CCCGC                CCCGC(4/6)
              158  CCCGC               
FokI          403  GGATG                GGATG(9/13)
              420  GGATG               
HhaI          134  GCGC                 GCG^C
              155  GCGC                
HinP1I        134  GCGC                 G^CGC
              155  GCGC                
HinfI          72  GATTC                G^A[ACGT]TC
              232  GATTC               
MaeII          32  ACGT                 A^CGT
               90  ACGT                
MseI          266  TTAA                 T^TAA
              310  TTAA                
MspA1I          9  CCGCTG               C[AC]G^C[GT]G
              103  CCGCTG              
NlaIII         96  CATG                 CATG^
              417  CATG                
PstI           55  CTGCAG               CTGCA^G
              194  CTGCAG              
RsaI          261  GTAC                 GT^AC
              283  GTAC                
ScrFI          36  CCTGG                CC^[ACGT]GG
              130  CCCGG               
SfaNI         622  GATGC                GCATC(5/9)
              161  GCATC               
SfcI           55  CTGCAG               C^T[GA][CT]AG
              194  CTGCAG              
TfiI           72  GATTC                G^A[AT]TC
              232  GATTC               
Tru9I         266  TTAA                 T^TAA
              310  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BsrFI       Cac8I       Cfr10I      Cfr13I      
DdeI        Fnu4HI      HaeIII      HapII       HpaII       
ItaI        MboII       MnlI        MspI        MwoI        
NaeI        NgoMIV      NlaIV       Sau96I      TseI        
Tsp509I     
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