Restriction map for pF1KB6930
 (Linear) (Six-base) MAPSORT of: KIBB6930.seq  Check: 4392  from: 1  to: 684



KIBB6930.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     684 
   Size:        547     137
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      40     684 
   Size:         40     644
 
ApoI r'AATT_y

Cuts at:      0     358     548     684 
   Size:        358     190     136

  Fragments arranged by size: 

                 358     190     136
 
BanI G'GyrC_C

Cuts at:      0     133     684 
   Size:        133     551
 
BbeI G_GCGC'C

Cuts at:      0     137     684 
   Size:        137     547
 
BlpI GC'TnA_GC

Cuts at:      0       4     684 
   Size:          4     680
 
BmgBI CAC'GTC

Cuts at:      0      33      91     684 
   Size:         33      58     593

  Fragments arranged by size: 

                 593      58      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     144     684 
   Size:        144     540
 
Bpu10I CC'TnA_GC

Cuts at:      0      43     684 
   Size:         43     641
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     684 
   Size:        511     173
 
BsaBI GATnn'nnATC

Cuts at:      0     298     684 
   Size:        298     386
 
BsaHI Gr'CG_yC

Cuts at:      0     134     684 
   Size:        134     550
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     684 
   Size:         67     617
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     682     684 
   Size:        682       2
 
BsiEI CG_ry'CG

Cuts at:      0      70     684 
   Size:         70     614
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      97     684 
   Size:         97     587
 
BspHI T'CATG_A

Cuts at:      0      95     684 
   Size:         95     589
 
BsrFI r'CCGG_y

Cuts at:      0      20      65     136     684 
   Size:         20      45      71     548

  Fragments arranged by size: 

                 548      71      45      20
 
BtgI C'CryG_G

Cuts at:      0     416     684 
   Size:        416     268
 
EaeI y'GGCC_r

Cuts at:      0      67     684 
   Size:         67     617
 
EagI C'GGCC_G

Cuts at:      0      67     684 
   Size:         67     617
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     684 
   Size:        547     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     146     684 
   Size:        146     538
 
EcoRI G'AATT_C

Cuts at:      0     548     684 
   Size:        548     136
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     412     684 
   Size:        412     272
 
HaeII r_GCGC'y

Cuts at:      0     137     684 
   Size:        137     547
 
HincII GTy'rAC

Cuts at:      0     569     684 
   Size:        569     115
 
KasI G'GCGC_C

Cuts at:      0     133     684 
   Size:        133     551
 
MspA1I CmG'CkG

Cuts at:      0      11     105     684 
   Size:         11      94     579

  Fragments arranged by size: 

                 579      94      11
 
NaeI GCC'GGC

Cuts at:      0      22      67     138     684 
   Size:         22      45      71     546

  Fragments arranged by size: 

                 546      71      45      22
 
NarI GG'CG_CC

Cuts at:      0     134     684 
   Size:        134     550
 
NcoI C'CATG_G

Cuts at:      0     416     684 
   Size:        416     268
 
NgoMIV G'CCGG_C

Cuts at:      0      20      65     136     684 
   Size:         20      45      71     548

  Fragments arranged by size: 

                 548      71      45      20
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36     684 
   Size:         36     648
 
PpuMI rG'GwC_Cy

Cuts at:      0     146     684 
   Size:        146     538
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     428     460     684 
   Size:        428      32     224

  Fragments arranged by size: 

                 428     224      32
 
PstI C_TGCA'G

Cuts at:      0      59     198     684 
   Size:         59     139     486

  Fragments arranged by size: 

                 486     139      59
 
SanDI GG'GwC_CC

Cuts at:      0     146     684 
   Size:        146     538
 
SfcI C'TryA_G

Cuts at:      0      55     194     684 
   Size:         55     139     490

  Fragments arranged by size: 

                 490     139      55
 
SfoI GGC'GCC

Cuts at:      0     135     684 
   Size:        135     549
 
SmlI C'TyrA_G

Cuts at:      0     490     684 
   Size:        490     194
 
StyI C'CwwG_G

Cuts at:      0     416     684 
   Size:        416     268
 
TatI w'GTAC_w

Cuts at:      0     260     684 
   Size:        260     424
 
XmnI GAAnn'nnTTC

Cuts at:      0     358     684 
   Size:        358     326
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BanI     BbeI     BlpI    BmgBI     BmrI
   Bpu10I    BpuEI    BsaBI    BsaHI    BseRI     BsgI    BsiEI    BsmBI
    BspHI    BsrFI     BtgI     EaeI     EagI  Eco57MI EcoO109I    EcoRI
     FalI    HaeII   HincII     KasI   MspA1I     NaeI     NarI     NcoI
   NgoMIV    PflMI    PpuMI     PsrI     PstI    SanDI     SfcI     SfoI
     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaWI    BsaXI    BseYI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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