Restriction map for pF1KB6929
 (Linear) (Six-base) MAPSORT of: KIBB6929.seq  Check: 462  from: 1  to: 621



KIBB6929.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     368     621 
   Size:        368     253
 
Acc65I G'GTAC_C

Cuts at:      0     492     621 
   Size:        492     129
 
AhdI GACnn_n'nnGTC

Cuts at:      0     227     621 
   Size:        227     394
 
AlwNI CAG_nnn'CTG

Cuts at:      0     617     621 
   Size:        617       4
 
ApaI G_GGCC'C

Cuts at:      0     409     532     621 
   Size:        409     123      89

  Fragments arranged by size: 

                 409     123      89
 
ApaLI G'TGCA_C

Cuts at:      0     376     621 
   Size:        376     245
 
AvaI C'yCGr_G

Cuts at:      0      61      69     621 
   Size:         61       8     552

  Fragments arranged by size: 

                 552      61       8
 
BamHI G'GATC_C

Cuts at:      0      56     621 
   Size:         56     565
 
BanI G'GyrC_C

Cuts at:      0      83     492     621 
   Size:         83     409     129

  Fragments arranged by size: 

                 409     129      83
 
BanII G_rGCy'C

Cuts at:      0     409     532     553     572     621 
   Size:        409     123      21      19      49

  Fragments arranged by size: 

                 409     123      49      21      19
 
BbeI G_GCGC'C

Cuts at:      0      87     621 
   Size:         87     534
 
Bme1580I G_kGCm'C

Cuts at:      0     380     409     532     621 
   Size:        380      29     123      89

  Fragments arranged by size: 

                 380     123      89      29
 
BmgBI CAC'GTC

Cuts at:      0      43     621 
   Size:         43     578
 
BsaHI Gr'CG_yC

Cuts at:      0      84     182     621 
   Size:         84      98     439

  Fragments arranged by size: 

                 439      98      84
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     621 
   Size:        271     350
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     415     621 
   Size:        316      99     206

  Fragments arranged by size: 

                 316     206      99
 
BsiEI CG_ry'CG

Cuts at:      0     455     621 
   Size:        455     166
 
BsiHKAI G_wGCw'C

Cuts at:      0      16     380     572     621 
   Size:         16     364     192      49

  Fragments arranged by size: 

                 364     192      49      16
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     380     409     532     553     572     621 
   Size:         16     364      29     123      21      19      49

  Fragments arranged by size: 

                 364     123      49      29      21      19      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     368     437     621 
   Size:        368      69     184

  Fragments arranged by size: 

                 368     184      69
 
BsrFI r'CCGG_y

Cuts at:      0     450     621 
   Size:        450     171
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     546     621 
   Size:        546      75
 
BstYI r'GATC_y

Cuts at:      0      56     206     621 
   Size:         56     150     415

  Fragments arranged by size: 

                 415     150      56
 
Bsu36I CC'TnA_GG

Cuts at:      0      32     621 
   Size:         32     589
 
BtgI C'CryG_G

Cuts at:      0     524     545     621 
   Size:        524      21      76

  Fragments arranged by size: 

                 524      76      21
 
BtsI GCAGTG_nn'

Cuts at:      0     573     621 
   Size:        573      48
 
DraIII CAC_nnn'GTG

Cuts at:      0     525     621 
   Size:        525      96
 
EaeI y'GGCC_r

Cuts at:      0     371     452     621 
   Size:        371      81     169

  Fragments arranged by size: 

                 371     169      81
 
EagI C'GGCC_G

Cuts at:      0     452     621 
   Size:        452     169
 
EcoICRI GAG'CTC

Cuts at:      0     570     621 
   Size:        570      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0      36     405     406     529     621 
   Size:         36     369       1     123      92

  Fragments arranged by size: 

                 369     123      92      36       1
 
FspI TGC'GCA

Cuts at:      0     604     621 
   Size:        604      17
 
HaeII r_GCGC'y

Cuts at:      0      87     143     426     621 
   Size:         87      56     283     195

  Fragments arranged by size: 

                 283     195      87      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     245     621 
   Size:        213      32     376

  Fragments arranged by size: 

                 376     213      32
 
KasI G'GCGC_C

Cuts at:      0      83     621 
   Size:         83     538
 
KpnI G_GTAC'C

Cuts at:      0     496     621 
   Size:        496     125
 
MslI CAynn'nnrTG

Cuts at:      0     362     544     562     621 
   Size:        362     182      18      59

  Fragments arranged by size: 

                 362     182      59      18
 
MspA1I CmG'CkG

Cuts at:      0      81     614     621 
   Size:         81     533       7

  Fragments arranged by size: 

                 533      81       7
 
NaeI GCC'GGC

Cuts at:      0     452     621 
   Size:        452     169
 
NarI GG'CG_CC

Cuts at:      0      84     621 
   Size:         84     537
 
NgoMIV G'CCGG_C

Cuts at:      0     450     621 
   Size:        450     171
 
NspI r_CATG'y

Cuts at:      0     367     621 
   Size:        367     254
 
PasI CC'CwG_GG

Cuts at:      0     243     621 
   Size:        243     378
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     525     621 
   Size:        525      96
 
PspOMI G'GGCC_C

Cuts at:      0     405     528     621 
   Size:        405     123      93

  Fragments arranged by size: 

                 405     123      93
 
SacI G_AGCT'C

Cuts at:      0     572     621 
   Size:        572      49
 
SfoI GGC'GCC

Cuts at:      0      85     621 
   Size:         85     536
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      31     621 
   Size:         31     590
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      41      73     621 
   Size:         41      32     548

  Fragments arranged by size: 

                 548      41      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     133     621 
   Size:        133     488
 

 Enzymes that do cut:

     AarI   Acc65I     AhdI    AlwNI     ApaI    ApaLI     AvaI    BamHI
     BanI    BanII     BbeI Bme1580I    BmgBI    BsaHI    BseRI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EagI  EcoICRI EcoO109I     FspI
    HaeII    Hin4I     KasI     KpnI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI    PflMI   PspOMI     SacI     SfoI    TaqII
     TstI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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