Restriction map for pF1KB6925
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglII       BlnI        
BmyI        BpmI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      Cac8I       ClaI        CpoI        DraI        
DraII       DraIII      DrdI        Eam1105I    EarI        
Eco47III    Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HpaI        KpnI        Ksp632I     MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SapI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          106  GTCGAC               GT^[AC][GT]AC
AccII          97  CGCG                 CG^CG
AseI          477  ATTAAT               AT^TAAT
AsnI          477  ATTAAT               AT^TAAT
BanI          285  GGCGCC               G^G[CT][GA]CC
BbeI          285  GGCGCC               GGCGC^C
BfaI          466  CTAG                 C^TAG
BglI            7  GCCTCGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          117  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      117  GCTGAGC              GC^T[ACGT]AGC
BsiEI          83  CGGCCG               CG[GA][CT]^CG
BsmAI         526  GAGAC                GTCTC(1/5)
BsrFI          23  ACCGGC               [GA]^CCGG[CT]
BssKI         302  CCAGG                ^CC[ACGT]GG
BstNI         302  CCAGG                CC^[AT]GG
BstUI          97  CGCG                 CG^CG
CelII         117  GCTGAGC              GC^T[ACGT]AGC
CfoI          286  GCGC                 GCG^C
Cfr10I         23  ACCGGC               [GA]^CCGG[CT]
Cfr13I         26  GGCCC                G^G[ACGT]CC
EaeI           83  CGGCCG               [CT]^GGCC[GA]
EagI           83  CGGCCG               C^GGCCG
EclXI          83  CGGCCG               C^GGCCG
Eco52I         83  CGGCCG               C^GGCCG
EcoRII        302  CCAGG                ^CC[AT]GG
EcoT14I       281  CCATGG               C^C[AT][AT]GG
HaeII         285  GGCGCC               [GA]GCGC^[CT]
HapII          24  CCGG                 C^CGG
HgaI           79  GCGTC                GACGC(5/10)
HhaI          286  GCGC                 GCG^C
HinP1I        286  GCGC                 G^CGC
HincII        106  GTCGAC               GT[CT]^[GA]AC
HindII        106  GTCGAC               GT[CT]^[GA]AC
HindIII       433  AAGCTT               A^AGCTT
HpaII          24  CCGG                 C^CGG
HphI          388  TCACC                GGTGA(8/7)
KasI          285  GGCGCC               G^GCGCC
KspI           96  CCGCGG               CCGC^GG
MaeI          466  CTAG                 C^TAG
MaeII         163  ACGT                 A^CGT
MspI           24  CCGG                 C^CGG
MvaI          302  CCAGG                CC^[AT]GG
MvnI           97  CGCG                 CG^CG
NarI          285  GGCGCC               GG^CGCC
NcoI          281  CCATGG               C^CATGG
PleI          452  GACTC                GAGTC(4/5)
PshBI         477  ATTAAT               AT^TAAT
PvuII         317  CAGCTG               CAG^CTG
SacII          96  CCGCGG               CCGC^GG
SalI          106  GTCGAC               G^TCGAC
Sau96I         26  GGCCC                G^G[ACGT]CC
ScrFI         302  CCAGG                CC^[ACGT]GG
SfaNI         250  GATGC                GCATC(5/9)
SfiI            6  GGCCTCGACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          285  GGCGCC               GGC^GCC
StyI          281  CCATGG               C^C[AT][AT]GG
XcmI          272  CCACTGAAGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI           78  GGCGTC               G[GA]^CG[CT]C
              285  GGCGCC              
AlwI           38  GGATC                GGATC(4/5)
              571  GGATC               
BbvI          316  GCAGC                GCAGC(8/12)
              313  GCTGC               
BsaHI          78  GGCGTC               G[GA]^CG[CT]C
              285  GGCGCC              
BseRI         112  GAGGAG               GAGGAG(10/8)
              370  GAGGAG              
BstYI          38  GGATCT               [GA]^GATC[CT]
              571  GGATCT              
DdeI          118  CTGAG                C^T[ACGT]AG
              297  CTTAG               
DpnII          39  GATC                 ^GATC
              572  GATC                
DsaI           96  CCGCGG               C^C[GA][CT]GG
              281  CCATGG              
FauI           95  CCCGC                CCCGC(4/6)
               98  GCGGG               
Hin1I          78  GGCGTC               G[GA]^CG[CT]C
              285  GGCGCC              
MboI           39  GATC                 ^GATC
              572  GATC                
MflI           38  GGATCT               [GA]^GATC[CT]
              571  GGATCT              
MseI          193  TTAA                 T^TAA
              478  TTAA                
MslI          439  CACTACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              496  CATGATGATG          
MspA1I         96  CCGCGG               C[AC]G^C[GT]G
              317  CAGCTG              
MwoI            7  GCCTCGACGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              271  GCCACTGAAGC         
NdeII          39  GATC                 ^GATC
              572  GATC                
NlaIV         285  GGCGCC               GG[ACGT]^[ACGT]CC
              330  GGAGCC              
PstI          314  CTGCAG               CTGCA^G
              530  CTGCAG              
Sau3AI         39  GATC                 ^GATC
              572  GATC                
TaqI           10  TCGA                 T^CGA
              107  TCGA                
TfiI          345  GATTC                G^A[AT]TC
              559  GAATC               
Tru9I         193  TTAA                 T^TAA
              478  TTAA                
TseI          313  GCTGC                G^C[AT]GC
              316  GCAGC               
TspRI         273  CACTG                CA[GC]TG[ACGT][ACGT]^
              363  CAGTG               
TthHB8I        10  TCGA                 T^CGA
              107  TCGA                
XhoII          38  GGATCT               [GA]^GATC[CT]
              571  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AciI        AluI        BsaJI       BsiYI       BslI        
BstF5I      Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MaeIII      MboII       MnlI        NlaIII      
SfcI        Tsp45I      Tsp509I     
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