Restriction map for pF1KB6925
 (Linear) (Six-base) MAPSORT of: KIBB6925.seq  Check: 6504  from: 1  to: 615



KIBB6925.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     107     615 
   Size:        107     508
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     341     413     615 
   Size:        296      45      72     202

  Fragments arranged by size: 

                 296     202      72      45
 
AseI AT'TA_AT

Cuts at:      0     478     615 
   Size:        478     137
 
BanI G'GyrC_C

Cuts at:      0     285     615 
   Size:        285     330
 
BbeI G_GCGC'C

Cuts at:      0     289     615 
   Size:        289     326
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13     615 
   Size:         13     602
 
BlpI GC'TnA_GC

Cuts at:      0     118     615 
   Size:        118     497
 
BsaHI Gr'CG_yC

Cuts at:      0      79     286     615 
   Size:         79     207     329

  Fragments arranged by size: 

                 329     207      79
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     322     352     615 
   Size:        322      30     263

  Fragments arranged by size: 

                 322     263      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     385     615 
   Size:        127     258     230

  Fragments arranged by size: 

                 258     230     127
 
BsiEI CG_ry'CG

Cuts at:      0      86     615 
   Size:         86     529
 
BsrFI r'CCGG_y

Cuts at:      0      23     615 
   Size:         23     592
 
BstYI r'GATC_y

Cuts at:      0      38     571     615 
   Size:         38     533      44

  Fragments arranged by size: 

                 533      44      38
 
BtgI C'CryG_G

Cuts at:      0      96     281     615 
   Size:         96     185     334

  Fragments arranged by size: 

                 334     185      96
 
BtsI GCAGTG_nn'

Cuts at:      0     369     615 
   Size:        369     246
 
EaeI y'GGCC_r

Cuts at:      0      83     615 
   Size:         83     532
 
EagI C'GGCC_G

Cuts at:      0      83     615 
   Size:         83     532
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     341     413     615 
   Size:        296      45      72     202

  Fragments arranged by size: 

                 296     202      72      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      57     236     615 
   Size:         57     179     379

  Fragments arranged by size: 

                 379     179      57
 
HaeII r_GCGC'y

Cuts at:      0     289     615 
   Size:        289     326
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     153     185     443     475     615 
   Size:        153      32     258      32     140

  Fragments arranged by size: 

                 258     153     140      32      32
 
HincII GTy'rAC

Cuts at:      0     108     615 
   Size:        108     507
 
HindIII A'AGCT_T

Cuts at:      0     433     615 
   Size:        433     182
 
KasI G'GCGC_C

Cuts at:      0     285     615 
   Size:        285     330
 
MslI CAynn'nnrTG

Cuts at:      0     443     500     615 
   Size:        443      57     115

  Fragments arranged by size: 

                 443     115      57
 
MspA1I CmG'CkG

Cuts at:      0      98     319     615 
   Size:         98     221     296

  Fragments arranged by size: 

                 296     221      98
 
NarI GG'CG_CC

Cuts at:      0     286     615 
   Size:        286     329
 
NcoI C'CATG_G

Cuts at:      0     281     615 
   Size:        281     334
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      40      72     615 
   Size:         40      32     543

  Fragments arranged by size: 

                 543      40      32
 
PstI C_TGCA'G

Cuts at:      0     318     534     615 
   Size:        318     216      81

  Fragments arranged by size: 

                 318     216      81
 
PvuII CAG'CTG

Cuts at:      0     319     615 
   Size:        319     296
 
SacII CC_GC'GG

Cuts at:      0      99     615 
   Size:         99     516
 
SalI G'TCGA_C

Cuts at:      0     106     615 
   Size:        106     509
 
SfcI C'TryA_G

Cuts at:      0     255     314     530     615 
   Size:        255      59     216      85

  Fragments arranged by size: 

                 255     216      85      59
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      13     615 
   Size:         13     602
 
SfoI GGC'GCC

Cuts at:      0     287     615 
   Size:        287     328
 
StyI C'CwwG_G

Cuts at:      0     281     615 
   Size:        281     334
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279     615 
   Size:        279     336
 

 Enzymes that do cut:

     AccI     AcuI     AseI     BanI     BbeI     BglI     BlpI    BsaHI
    BsaXI    BseRI    BsiEI    BsrFI    BstYI     BtgI     BtsI     EaeI
     EagI  Eco57MI     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     MslI   MspA1I     NarI     NcoI     PpiI     PstI    PvuII    SacII
     SalI     SfcI     SfiI     SfoI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SanDI     SapI     SbfI
     ScaI    SexAI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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