Restriction map for pF1KB6917
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiHKAI     BsiWI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DrdI        
Eam1105I    Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        HphI        KpnI        
KspI        MaeI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SexAI       SfcI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          223  GTCGAC               GT^[AC][GT]AC
AlwI          291  GGATC                GGATC(4/5)
AlwNI         502  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       578  AGCGCT               AGC^GCT
Asp700I        39  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          485  CCCGGG               C^[CT]CG[GA]G
BbsI          439  GAAGAC               GAAGAC(2/6)
BglI          133  GCCGCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         439  GAAGAC               GAAGAC(2/6)
BseRI         427  GAGGAG               GAGGAG(10/8)
BsmAI         125  GAGAC                GTCTC(1/5)
BsmBI         125  GAGACG               CGTCTC(1/5)
BsmFI         260  GGGAC                GGGAC(10/14)
BsoBI         485  CCCGGG               C^[CT]CG[GA]G
BsrBI          16  GAGCGG               CCGCTC(-3/-3)
DpnII         292  GATC                 ^GATC
DraIII        498  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           30  CCACGG               C^C[GA][CT]GG
EarI           43  CTCTTC               CTCTTC(1/4)
Eco47III      578  AGCGCT               AGC^GCT
EcoNI         194  CCTCCCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        223  GTCGAC               GT[CT]^[GA]AC
HindII        223  GTCGAC               GT[CT]^[GA]AC
HinfI         172  GATTC                G^A[ACGT]TC
Ksp632I        43  CTCTTC               CTCTTC(1/4)
MaeII         235  ACGT                 A^CGT
MaeIII        611  GTGAC                ^GT[ACGT]AC
MboI          292  GATC                 ^GATC
MspA1I        315  CAGCTG               C[AC]G^C[GT]G
NdeII         292  GATC                 ^GATC
NotI          130  GCGGCCGC             GC^GGCCGC
NruI          294  TCGCGA               TCG^CGA
NspI          490  GCATGC               [GA]CATG^[CT]
PflMI         330  CCACACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         315  CAGCTG               CAG^CTG
SalI          223  GTCGAC               G^TCGAC
Sau3AI        292  GATC                 ^GATC
SfaNI         240  GATGC                GCATC(5/9)
SfiI          132  GGCCGCCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          485  CCCGGG               CCC^GGG
SphI          490  GCATGC               GCATG^C
TfiI          172  GATTC                G^A[AT]TC
Tsp45I        611  GTGAC                ^GT[GC]AC
TspRI         502  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        330  CCACACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         485  CCCGGG               C^CCGGG
XmaI          485  CCCGGG               C^CCGGG
XmnI           39  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AluI          316  AGCT                 AG^CT
              539  AGCT                
BanI            6  GGCGCC               G^G[CT][GA]CC
              159  GGCGCC              
BbeI            6  GGCGCC               GGCGC^C
              159  GGCGCC              
BbvI          249  GCAGC                GCAGC(8/12)
              375  GCAGC               
BsgI          517  CTGCAC               GTGCAG(16/14)
              459  GTGCAG              
BspMI         356  ACCTGC               ACCTGC(4/8)
              515  ACCTGC              
BsrI          303  ACTGG                ACTGG(1/-1)
              390  CCAGT               
BssHII         62  GCGCGC               G^CGCGC
               64  GCGCGC              
DdeI          410  CTAAG                C^T[ACGT]AG
              445  CTAAG               
EagI          131  CGGCCG               C^GGCCG
              205  CGGCCG              
EclXI         131  CGGCCG               C^GGCCG
              205  CGGCCG              
Eco52I        131  CGGCCG               C^GGCCG
              205  CGGCCG              
KasI            6  GGCGCC               G^GCGCC
              159  GGCGCC              
NarI            6  GGCGCC               GG^CGCC
              159  GGCGCC              
NlaIII        384  CATG                 CATG^
              491  CATG                
SfoI            6  GGCGCC               GGC^GCC
              159  GGCGCC              
TseI          249  GCAGC                G^C[AT]GC
              375  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AccII       AciI        AcyI        BcnI        BsaHI       
BsaJI       BsiEI       BsiYI       BslI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
TaqI        TthHB8I     
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