Restriction map for pF1KB6913
 (Circular) (Six-base) MAPSORT of: pF1KB6913.seq  Check: 4059  from: 1  to: 3728



pF1KB6913  3728 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    766     766 
   Size:       3728
 
Acc65I G'GTAC_C

Cuts at:    744     744 
   Size:       3728
 
AclI AA'CG_TT

Cuts at:   3568    3568 
   Size:       3728
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1553    1985    2732    3000    1553 
   Size:        432     747     268    2281

  Fragments arranged by size: 

                2281     747     432     268
 
AflIII A'CryG_T

Cuts at:   1080    2184    3161    1080 
   Size:       1104     977    1647

  Fragments arranged by size: 

                1647    1104     977
 
AgeI A'CCGG_T

Cuts at:   2116    2116 
   Size:       3728
 
AlwNI CAG_nnn'CTG

Cuts at:   1033    2600    1033 
   Size:       1567    2161
 
ApaLI G'TGCA_C

Cuts at:   2498    2498 
   Size:       3728
 
ApoI r'AATT_y

Cuts at:    457     732    3081    3337     457 
   Size:        275    2349     256     848

  Fragments arranged by size: 

                2349     848     275     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3728
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3728
 
AvaI C'yCGr_G

Cuts at:    748     748 
   Size:       3728
 
BamHI G'GATC_C

Cuts at:    753     753 
   Size:       3728
 
BanI G'GyrC_C

Cuts at:    744    1158    1406    1441     744 
   Size:        414     248      35    3031

  Fragments arranged by size: 

                3031     414     248      35
 
BanII G_rGCy'C

Cuts at:    742    1772     742 
   Size:       1030    2698
 
BbeI G_GCGC'C

Cuts at:   1162    1410    1162 
   Size:        248    3480
 
BciVI GTATCCnnnnn_n'

Cuts at:   1626    2387    3728    1626 
   Size:        761    1341    1626

  Fragments arranged by size: 

                1626    1341     761
 
BclI T'GATC_A

Cuts at:   1248    1248 
   Size:       3728
 
BglII A'GATC_T

Cuts at:    446    1243    3280     446 
   Size:        797    2037     894

  Fragments arranged by size: 

                2037     894     797
 
BlpI GC'TnA_GC

Cuts at:    840     840 
   Size:       3728
 
Bme1580I G_kGCm'C

Cuts at:   1353    1446    2502    1353 
   Size:         93    1056    2579

  Fragments arranged by size: 

                2579    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    940    1021    1356    3500     940 
   Size:         81     335    2144    1168

  Fragments arranged by size: 

                2144    1168     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2095    2095 
   Size:       3728
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    165     598     904    1783    2275    2573     165 
   Size:        433     306     879     492     298    1320

  Fragments arranged by size: 

                1320     879     492     433     306     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3432    3432 
   Size:       3728
 
BsaAI yAC'GTr

Cuts at:   1081    1081 
   Size:       3728
 
BsaBI GATnn'nnATC

Cuts at:      6    1242    3279       6 
   Size:       1236    2037     455

  Fragments arranged by size: 

                2037    1236     455
 
BsaHI Gr'CG_yC

Cuts at:   1159    1407    2109    3605    1159 
   Size:        248     702    1496    1282

  Fragments arranged by size: 

                1496    1282     702     248
 
BsaWI w'CCGG_w

Cuts at:    917    1438    2116    2390    2537    2948     917 
   Size:        521     678     274     147     411    1697

  Fragments arranged by size: 

                1697     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    162     379     162 
   Size:        217    3511
 
BseYI C'CCAG_C

Cuts at:   1153    1911    2488    2984    1153 
   Size:        758     577     496    1897

  Fragments arranged by size: 

                1897     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1270    1316    2524     104 
   Size:       1166      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1166      46
 
BsiHKAI G_wGCw'C

Cuts at:    742    1520    1710    2502     742 
   Size:        778     190     792    1968

  Fragments arranged by size: 

                1968     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    742    1353    1446    1520    1710    1772    2502     742 
   Size:        611      93      74     190      62     730    1968

  Fragments arranged by size: 

                1968     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2948    2948 
   Size:       3728
 
BspHI T'CATG_A

Cuts at:    312    2904     312 
   Size:       2592    1136
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    115     556     778     992    1294    1675     115 
   Size:        441     222     214     302     381    2168

  Fragments arranged by size: 

                2168     441     381     302     222     214
 
BsrBI CCG'CTC

Cuts at:     50    2020    2074    3558    3723      50 
   Size:       1970      54    1484     165      55

  Fragments arranged by size: 

                1970    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1640    1640 
   Size:       3728
 
BsrFI r'CCGG_y

Cuts at:    132    1726    1907    2116     132 
   Size:       1594     181     209    1744

  Fragments arranged by size: 

                1744    1594     209     181
 
BssSI C'ACGA_G

Cuts at:   1999    2357    1999 
   Size:        358    3370
 
BstAPI GCAn_nnn'nTGC

Cuts at:    697    1105     697 
   Size:        408    3320
 
BstBI TT'CG_AA

Cuts at:   2089    2089 
   Size:       3728
 
BstYI r'GATC_y

Cuts at:      1     446     753    1220    1243    1578    1824    2836    2922 
   Size:        445     307     467      23     335     246    1012      86

Cuts at:   2922    2934    3280       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     445     346     335     307     246
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1678    1859    2022    1678 
   Size:        181     163    3384

  Fragments arranged by size: 

                3384     181     163
 
BtsI GCAGTG_nn'

Cuts at:    971     971 
   Size:       3728
 
DraI TTT'AAA

Cuts at:    727     727 
   Size:       3728
 
DrdI GACnn_nn'nnGTC

Cuts at:   1434    2292    1434 
   Size:        858    2870
 
EaeI y'GGCC_r

Cuts at:   1313    1487    1878    1905    3250    1313 
   Size:        174     391      27    1345    1791

  Fragments arranged by size: 

                1791    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1313    1313 
   Size:       3728
 
EarI CTCTTCn'nnn_

Cuts at:    389     506    1751    1961     389 
   Size:        117    1245     210    2156

  Fragments arranged by size: 

                2156    1245     210     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1936    2246    2392    3538    1936 
   Size:        310     146    1146    2126

  Fragments arranged by size: 

                2126    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    740     740 
   Size:       3728
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1553    1985    2732    3000    1553 
   Size:        432     747     268    2281

  Fragments arranged by size: 

                2281     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    867     867 
   Size:       3728
 
EcoRI G'AATT_C

Cuts at:    457     732     457 
   Size:        275    3453
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1747    1747 
   Size:       3728
 
FspI TGC'GCA

Cuts at:   1509    1509 
   Size:       3728
 
HaeII r_GCGC'y

Cuts at:   1162    1410    2432    3411    1162 
   Size:        248    1022     979    1479

  Fragments arranged by size: 

                1479    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1570    1600    1602    1632    1570 
   Size:         30       2      30    3666

  Fragments arranged by size: 

                3666      30      30       2
 
HincII GTy'rAC

Cuts at:    642     767     642 
   Size:        125    3603
 
HindIII A'AGCT_T

Cuts at:    338     338 
   Size:       3728
 
KasI G'GCGC_C

Cuts at:   1158    1406    1158 
   Size:        248    3480
 
KpnI G_GTAC'C

Cuts at:    748     748 
   Size:       3728
 
MluI A'CGCG_T

Cuts at:   3161    3161 
   Size:       3728
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2399    2583    2399 
   Size:        184    3544
 
MscI TGG'CCA

Cuts at:   1489    1489 
   Size:       3728
 
MslI CAynn'nnrTG

Cuts at:    317    1717    1844     317 
   Size:       1400     127    2201

  Fragments arranged by size: 

                2201    1400     127
 
MspA1I CmG'CkG

Cuts at:    389     653     839    1153    1513    2526    2771     389 
   Size:        264     186     314     360    1013     245    1346

  Fragments arranged by size: 

                1346    1013     360     314     264     245     186
 
NaeI GCC'GGC

Cuts at:    134    1909     134 
   Size:       1775    1953
 
NarI GG'CG_CC

Cuts at:   1159    1407    1159 
   Size:        248    3480
 
NgoMIV G'CCGG_C

Cuts at:    132    1907     132 
   Size:       1775    1953
 
NspI r_CATG'y

Cuts at:    531     781    1281    2188     531 
   Size:        250     500     907    2071

  Fragments arranged by size: 

                2071     907     500     250
 
PciI A'CATG_T

Cuts at:   2184    2184 
   Size:       3728
 
PmeI GTTT'AAAC

Cuts at:    727     727 
   Size:       3728
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2867    2899    2867 
   Size:         32    3696
 
PstI C_TGCA'G

Cuts at:    775    1460     775 
   Size:        685    3043
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3728
 
PvuII CAG'CTG

Cuts at:    389     653    1153    1513     389 
   Size:        264     500     360    2604

  Fragments arranged by size: 

                2604     500     360     264
 
RsrII CG'GwC_CG

Cuts at:   1923    1923 
   Size:       3728
 
SacI G_AGCT'C

Cuts at:    742     742 
   Size:       3728
 
SalI G'TCGA_C

Cuts at:    765     765 
   Size:       3728
 
SapI GCTCTTCn'nnn_

Cuts at:   1751    1961    1751 
   Size:        210    3518
 
SbfI CC_TGCA'GG

Cuts at:    775     775 
   Size:       3728
 
ScaI AGT'ACT

Cuts at:   3245    3245 
   Size:       3728
 
SfcI C'TryA_G

Cuts at:     33     771    1456    2449    2640      33 
   Size:        738     685     993     191    1121

  Fragments arranged by size: 

                1121     993     738     685     191
 
SfoI GGC'GCC

Cuts at:   1160    1408    1160 
   Size:        248    3480
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3728
 
SmaI CCC'GGG

Cuts at:    750     750 
   Size:       3728
 
SmlI C'TyrA_G

Cuts at:    144     613     883    1798    2290    2552     144 
   Size:        469     270     915     492     262    1320

  Fragments arranged by size: 

                1320     915     492     469     270     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3728
 
SphI G_CATG'C

Cuts at:    781    1281     781 
   Size:        500    3228
 
SspI AAT'ATT

Cuts at:    372     372 
   Size:       3728
 
StyI C'CwwG_G

Cuts at:    434     862    1216     434 
   Size:        428     354    2946

  Fragments arranged by size: 

                2946     428     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1311    1444    1903    2112    1311 
   Size:        133     459     209    2927

  Fragments arranged by size: 

                2927     459     209     133
 
TatI w'GTAC_w

Cuts at:    398    3243     398 
   Size:       2845     883
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    202     234     375     407    3245    3277     202 
   Size:         32     141      32    2838      32     653

  Fragments arranged by size: 

                2838     653     141      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1525    1525 
   Size:       3728
 
XbaI T'CTAG_A

Cuts at:    759     759 
   Size:       3728
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1223    1223 
   Size:       3728
 
XmaI C'CCGG_G

Cuts at:    748     748 
   Size:       3728
 
XmnI GAAnn'nnTTC

Cuts at:    574     574 
   Size:       3728
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
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