Restriction map for pF1KB6901
 (Linear) (Six-base) MAPSORT of: KIBB6901.seq  Check: 5327  from: 1  to: 609



KIBB6901.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AatII G_ACGT'C

Cuts at:      0     215     609 
   Size:        215     394
 
AclI AA'CG_TT

Cuts at:      0     459     609 
   Size:        459     150
 
ApoI r'AATT_y

Cuts at:      0     239     609 
   Size:        239     370
 
BanI G'GyrC_C

Cuts at:      0      93     609 
   Size:         93     516
 
BanII G_rGCy'C

Cuts at:      0      55     609 
   Size:         55     554
 
BbeI G_GCGC'C

Cuts at:      0      97     609 
   Size:         97     512
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     291     609 
   Size:        291     318
 
BclI T'GATC_A

Cuts at:      0     561     609 
   Size:        561      48
 
BfrBI ATG'CAT

Cuts at:      0     349     609 
   Size:        349     260
 
BmtI G_CTAG'C

Cuts at:      0     599     609 
   Size:        599      10
 
BsaHI Gr'CG_yC

Cuts at:      0      58      94     212     609 
   Size:         58      36     118     397

  Fragments arranged by size: 

                 397     118      58      36
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     202     232     609 
   Size:        202      30     377

  Fragments arranged by size: 

                 377     202      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     207     346     609 
   Size:        207     139     263

  Fragments arranged by size: 

                 263     207     139
 
BsiEI CG_ry'CG

Cuts at:      0     107     131     164     609 
   Size:        107      24      33     445

  Fragments arranged by size: 

                 445     107      33      24
 
BsmI GAATG_Cn'

Cuts at:      0     278     609 
   Size:        278     331
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     219     609 
   Size:        219     390
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     609 
   Size:         55     554
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     429     609 
   Size:        429     180
 
BsrGI T'GTAC_A

Cuts at:      0     245     609 
   Size:        245     364
 
BstYI r'GATC_y

Cuts at:      0     315     609 
   Size:        315     294
 
BtgI C'CryG_G

Cuts at:      0     131     609 
   Size:        131     478
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      90     609 
   Size:         90     519
 
EaeI y'GGCC_r

Cuts at:      0     104     128     134     161     609 
   Size:        104      24       6      27     448

  Fragments arranged by size: 

                 448     104      27      24       6
 
EagI C'GGCC_G

Cuts at:      0     104     128     161     609 
   Size:        104      24      33     448

  Fragments arranged by size: 

                 448     104      33      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     473     609 
   Size:        274     199     136

  Fragments arranged by size: 

                 274     199     136
 
HaeII r_GCGC'y

Cuts at:      0      97     609 
   Size:         97     512
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234     609 
   Size:        202      32     375

  Fragments arranged by size: 

                 375     202      32
 
KasI G'GCGC_C

Cuts at:      0      93     609 
   Size:         93     516
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     609 
   Size:        330     279
 
MspA1I CmG'CkG

Cuts at:      0      45     109     133     157     609 
   Size:         45      64      24      24     452

  Fragments arranged by size: 

                 452      64      45      24      24
 
NarI GG'CG_CC

Cuts at:      0      94     609 
   Size:         94     515
 
NheI G'CTAG_C

Cuts at:      0     595     609 
   Size:        595      14
 
NotI GC'GGCC_GC

Cuts at:      0     128     161     609 
   Size:        128      33     448

  Fragments arranged by size: 

                 448     128      33
 
NsiI A_TGCA'T

Cuts at:      0     351     609 
   Size:        351     258
 
NspI r_CATG'y

Cuts at:      0     349     609 
   Size:        349     260
 
PstI C_TGCA'G

Cuts at:      0     393     609 
   Size:        393     216
 
SacII CC_GC'GG

Cuts at:      0     134     609 
   Size:        134     475
 
SfcI C'TryA_G

Cuts at:      0     389     609 
   Size:        389     220
 
SfoI GGC'GCC

Cuts at:      0      95     609 
   Size:         95     514
 
SspI AAT'ATT

Cuts at:      0     509     609 
   Size:        509     100
 
TatI w'GTAC_w

Cuts at:      0     245     325     609 
   Size:        245      80     284

  Fragments arranged by size: 

                 284     245      80
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     438     470     609 
   Size:        438      32     139

  Fragments arranged by size: 

                 438     139      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     239     609 
   Size:        239     370
 
ZraI GAC'GTC

Cuts at:      0     213     609 
   Size:        213     396
 

 Enzymes that do cut:

    AatII     AclI     ApoI     BanI    BanII     BbeI     BbsI     BclI
    BfrBI     BmtI    BsaHI    BsaXI    BseRI    BsiEI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrGI    BstYI     BtgI    BtgZI     EaeI     EagI
     EarI    HaeII    Hin4I     KasI     MmeI   MspA1I     NarI     NheI
     NotI     NsiI     NspI     PstI    SacII     SfcI     SfoI     SspI
     TatI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI
     BsgI  BsiHKAI    BsiWI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
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